Detailed information    

experimental Experimentally validated

Overview

Name   htrA   Type   Regulator
Locus tag   SM12261_RS09395 Genome accession   NZ_CP028414
Coordinates   1866792..1867973 (+) Length   393 a.a.
NCBI ID   WP_000681587.1    Uniprot ID   A0A0F2DYC6
Organism   Streptococcus mitis NCTC 12261     
Function   repress competence development   
Competence regulation

Function

HtrA inhibits natural transformation of Streptococcus mitis and Streptococcus gordonii.


Genomic Context

Location: 1861792..1872973
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SM12261_RS09355 (SM12261_1805) - 1862853..1863395 (+) 543 WP_001158274.1 TetR/AcrR family transcriptional regulator -
  SM12261_RS09370 (SM12261_1808) comE 1863638..1864390 (-) 753 WP_000866073.1 competence system response regulator transcription factor ComE Regulator
  SM12261_RS09375 (SM12261_1809) comD 1864387..1865712 (-) 1326 WP_020902616.1 competence system sensor histidine kinase ComD Regulator
  SM12261_RS09380 (SM12261_1810) comC 1865725..1865847 (-) 123 WP_004238992.1 competence-stimulating peptide ComC Regulator
  SM12261_RS09390 (SM12261_1812) rlmH 1866130..1866609 (-) 480 WP_000695934.1 23S rRNA (pseudouridine(1915)-N(3))-methyltransferase RlmH -
  SM12261_RS09395 (SM12261_1813) htrA 1866792..1867973 (+) 1182 WP_000681587.1 S1C family serine protease Regulator
  SM12261_RS09400 (SM12261_1814) spo0J 1868031..1868789 (+) 759 WP_000410370.1 ParB/RepB/Spo0J family partition protein Regulator

Sequence

Protein


Download         Length: 393 a.a.        Molecular weight: 41729.49 Da        Isoelectric Point: 5.7805

>NTDB_id=539 SM12261_RS09395 WP_000681587.1 1866792..1867973(+) (htrA) [Streptococcus mitis NCTC 12261]
MKHLKTFYKKGFQLLVVIVISFFSGALGSFSINQLTQKSTESTSNNNSTITQTAYKNENSTTQAVNKVKDAVVSVITYSA
NRQNSVFGNDDTDTDSQQISSEGSGVIYKKNDKEAYIVTNNHVINGASKVDIRLSDGTKVPGEIVGADTFSDIAVVKISS
EKVTTVAEFGDSSKLTVGETAIAIGSPLGSEYANTVTQGIVSSLNRNVSLKSEDGQAISTKAIQTDTAINPGNSGGPLIN
IQGQVIGITSSKIATNGGTSVEGLGFAIPANDAINIIEQLEKNGKVTRPALGIQMVNLSNINTSDIRRLNIPSNVTSGVV
VRSVQSNMPANGHLEKYDVITKVDDKEIASSTDLQSALYNHSIGDTIKITYYRNGKEETTSIKLDKSSGDLES

Nucleotide


Download         Length: 1182 bp        

>NTDB_id=539 SM12261_RS09395 WP_000681587.1 1866792..1867973(+) (htrA) [Streptococcus mitis NCTC 12261]
ATGAAACATTTAAAAACATTTTACAAAAAAGGGTTTCAATTATTAGTCGTTATCGTCATTAGCTTTTTTAGTGGAGCCTT
GGGTAGCTTTTCAATAAACCAACTAACTCAAAAAAGTACTGAAAGTACCTCTAACAACAATAGTACTATTACACAGACTG
CCTATAAGAATGAAAATTCAACAACACAGGCTGTTAACAAAGTAAAAGATGCCGTTGTTTCTGTTATTACTTATTCAGCA
AACAGACAAAATAGCGTATTTGGTAATGATGATACTGACACAGATTCTCAGCAAATCTCTAGTGAAGGATCTGGGGTTAT
TTATAAAAAGAATGATAAAGAAGCTTACATCGTCACCAACAATCACGTTATAAATGGTGCCAGCAAAGTAGATATTCGTT
TGTCAGATGGAACTAAAGTACCTGGAGAAATCGTCGGAGCTGATACTTTCTCTGATATTGCTGTCGTCAAAATCTCTTCA
GAAAAAGTGACAACAGTAGCTGAATTTGGTGATTCTAGCAAGTTAACCGTAGGAGAAACTGCTATTGCCATCGGTAGCCC
ATTAGGTTCTGAATATGCTAATACTGTCACTCAAGGTATCGTATCTAGTCTTAATCGAAATGTATCTTTAAAATCTGAAG
ATGGACAAGCCATTTCTACAAAAGCCATCCAAACTGATACTGCTATTAACCCAGGTAACTCTGGTGGCCCACTAATCAAT
ATTCAAGGACAGGTTATCGGAATTACCTCAAGTAAAATTGCCACAAATGGAGGAACATCTGTAGAAGGTCTTGGTTTTGC
AATCCCTGCAAATGATGCTATCAATATTATTGAACAGTTGGAAAAGAACGGAAAAGTTACTCGTCCAGCTTTGGGAATTC
AAATGGTCAATCTCTCAAATATTAATACAAGTGATATTAGAAGACTGAATATTCCAAGCAATGTAACATCTGGTGTAGTT
GTTCGTTCTGTGCAAAGTAATATGCCTGCCAATGGTCACCTTGAAAAATACGATGTTATTACAAAAGTAGATGACAAGGA
AATTGCTTCATCAACAGACTTACAAAGTGCTCTTTACAATCATTCTATCGGAGATACCATTAAAATAACTTACTATCGTA
ACGGTAAAGAAGAAACTACATCTATTAAACTTGACAAGAGTTCAGGTGATTTAGAATCTTAA

Domains

Predicted by InterproScan.

PDZ domain (PDZ_2)

(289-384)

Trypsin-like peptidase domain (Trypsin_2)

(103-248)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0F2DYC6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  htrA Streptococcus pneumoniae TIGR4

97.201

100

0.972
  htrA Streptococcus pneumoniae D39

97.201

100

0.972
  htrA Streptococcus pneumoniae Rx1

97.201

100

0.972
  htrA Streptococcus pneumoniae R6

97.201

100

0.972
  htrA Streptococcus gordonii str. Challis substr. CH1

66.332

100

0.672
  htrA Streptococcus mutans UA159

54.684

100

0.55

Multiple sequence alignment    



References

[1] Yanni Liu et al. (2019) HtrA-mediated selective degradation of DNA uptake apparatus accelerates termination of pneumococcal transformation. Molecular Microbiology 112(4):1308-1325. [PMID: 31396996]