Detailed information    

experimental Experimentally validated

Overview

Name   htrA   Type   Regulator
Locus tag   SGO_RS10525 Genome accession   NC_009785
Coordinates   2194602..2195795 (+) Length   397 a.a.
NCBI ID   WP_012131081.1    Uniprot ID   A0AB34SBT8
Organism   Streptococcus gordonii str. Challis substr. CH1     
Function   repress competence development   
Competence regulation

Function

HtrA inhibits natural transformation of Streptococcus mitis and Streptococcus gordonii.


Genomic Context

Location: 2189602..2200795
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SGO_RS10485 (SGO_2142) ychF 2189913..2191028 (-) 1116 WP_012131077.1 redox-regulated ATPase YchF -
  SGO_RS10500 (SGO_2145) comE/comE1 2191350..2192117 (-) 768 WP_008808031.1 competence system response regulator transcription factor ComE Regulator
  SGO_RS10505 (SGO_2146) comD/comD1 2192114..2193475 (-) 1362 WP_012131078.1 competence system sensor histidine kinase ComD Regulator
  SGO_RS10510 (SGO_2147) comC/comC1 2193491..2193643 (-) 153 WP_012131079.1 bacteriocin Regulator
  SGO_RS10520 (SGO_2149) rlmH 2193929..2194408 (-) 480 WP_012131080.1 23S rRNA (pseudouridine(1915)-N(3))-methyltransferase RlmH -
  SGO_RS10525 (SGO_2150) htrA 2194602..2195795 (+) 1194 WP_012131081.1 S1C family serine protease Regulator
  SGO_RS10530 (SGO_2151) spo0J 2195861..2196625 (+) 765 WP_012131082.1 ParB/RepB/Spo0J family partition protein Regulator

Sequence

Protein


Download         Length: 397 a.a.        Molecular weight: 42183.77 Da        Isoelectric Point: 4.9802

>NTDB_id=425 SGO_RS10525 WP_012131081.1 2194602..2195795(+) (htrA) [Streptococcus gordonii str. Challis substr. CH1]
MKNSSNFFKKTLQVFIVLLVGFIGGVAGTWGFSYFSTPHSATNNNQKTATTVTTSYKNSNSTTEAVDKVKDAVVSVITYS
ESNSDAIFDNNNSGNENDQVASEGSGVIYRKDKDYAYLVTNTHVINGAKKVDIRLADGNKVPGEIVGTDTYSDISVVRIP
ADKVKNVAEFGDSSKLTVGETAIAIGSPLGSDYANTVTQGIVSSLSRNVSSRSEDGQTIATQAIQTDAAINPGNSGGPLV
NIQGQVIGITSSKIANTNNGSTSVEGMGFAIPSNDVVNIIEQLEKNGKVIRPALGIQMVNLSSLSSSSSDRLKLPDNVKN
GVVVRSTQTGMPADGKLEKYDVITKIDDTEISSASDIQSALYKHSINEEIKVTYYRDGKEQTTTIKLTKSTDDLSSE

Nucleotide


Download         Length: 1194 bp        

>NTDB_id=425 SGO_RS10525 WP_012131081.1 2194602..2195795(+) (htrA) [Streptococcus gordonii str. Challis substr. CH1]
ATGAAAAACTCTTCAAATTTCTTTAAAAAAACATTGCAAGTTTTCATTGTTTTGTTAGTTGGTTTTATAGGTGGTGTTGC
TGGAACATGGGGATTTTCTTATTTTTCAACACCTCATTCAGCCACAAATAATAATCAGAAAACAGCGACTACAGTAACAA
CATCCTATAAAAATAGTAATTCTACAACAGAGGCTGTTGATAAAGTAAAAGATGCGGTTGTCTCTGTCATTACATATTCT
GAATCTAATTCAGATGCTATTTTCGACAACAATAATTCAGGAAATGAAAATGATCAGGTAGCTAGCGAAGGATCTGGTGT
TATCTATAGAAAAGATAAGGATTATGCTTACTTAGTTACCAATACACACGTTATCAATGGAGCAAAAAAAGTTGATATCC
GTCTTGCTGATGGGAATAAGGTTCCTGGTGAAATAGTCGGGACTGATACATATTCTGATATTTCTGTAGTCAGAATTCCT
GCCGATAAAGTTAAAAATGTGGCAGAATTTGGGGATTCTAGTAAATTAACTGTTGGGGAAACTGCCATTGCAATCGGAAG
TCCTCTTGGATCTGATTATGCTAATACAGTGACTCAGGGAATTGTTTCTAGTCTAAGCCGTAATGTTTCTTCACGATCAG
AAGATGGTCAGACAATTGCTACTCAAGCTATTCAAACTGATGCAGCAATTAATCCAGGTAACTCTGGTGGTCCTCTTGTA
AACATTCAAGGTCAAGTTATTGGTATTACCTCTAGTAAAATTGCTAACACCAATAATGGTAGTACTTCTGTTGAGGGAAT
GGGATTTGCAATCCCATCCAATGATGTTGTAAATATCATTGAACAATTAGAAAAGAATGGTAAAGTCATTCGACCAGCAT
TAGGAATTCAAATGGTCAACTTAAGTAGTTTGTCTTCTTCAAGCTCTGATCGATTAAAACTTCCAGACAATGTTAAAAAT
GGTGTCGTTGTTCGTTCAACACAAACTGGAATGCCTGCTGATGGTAAGTTAGAAAAATATGATGTGATTACAAAAATTGA
TGATACAGAAATATCATCTGCTAGCGATATTCAATCAGCTCTTTATAAACATTCAATTAATGAAGAAATTAAAGTTACTT
ATTATCGTGATGGAAAAGAGCAAACAACAACTATTAAACTAACTAAATCAACTGATGATTTAAGCTCTGAATAA

Domains

Predicted by InterproScan.

PDZ domain (PDZ_2)

(292-387)

Peptidase family M50 (Peptidase_M50)

(236-391)

Trypsin-like peptidase domain (Trypsin_2)

(104-249)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  htrA Streptococcus mitis NCTC 12261

66.332

100

0.672
  htrA Streptococcus pneumoniae R6

66.414

100

0.669
  htrA Streptococcus pneumoniae D39

66.414

100

0.669
  htrA Streptococcus pneumoniae Rx1

66.414

100

0.669
  htrA Streptococcus pneumoniae TIGR4

66.414

100

0.669
  htrA Streptococcus mutans UA159

56.14

100

0.564

Multiple sequence alignment    



References

[1] Yanni Liu et al. (2019) HtrA-mediated selective degradation of DNA uptake apparatus accelerates termination of pneumococcal transformation. Molecular Microbiology 112(4):1308-1325. [PMID: 31396996]