Detailed information NTG0000381

Experimentally validated

Overview

• Name: htrA
• Type: Regulator
• Locus tag: SMU_RS09890
• Genome accession: NC_004350
• Coordinates: 2030461..2031669 (+)
• Length: 402 a.a.
• NCBI ID: WP_002262650.1
• Uniprot ID: Q8DRQ6
• Organism: Streptococcus mutans UA159
• Function: require for competence development Competence regulation

Function

The htrA mutant displayed a significantly reduced ability to undergo genetic transformation. A direct association between HtrA and genetic competence was demonstrated by the increased expression of the htrA gene upon exposure to competence-stimulating peptide.

Genomic Context

Location: 2025461..2036669

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
SMU_RS09880 (SMU.2161c)	-	2028771..2029433 (-)	663	WP_002262652.1	YoaK family protein	-
SMU_RS09885 (SMU.2162c)	rlmH	2029776..2030255 (-)	480	WP_002262651.1	23S rRNA (pseudouridine(1915)-N(3))-methyltransferase RlmH	-
SMU_RS09890 (SMU.2164)	htrA	2030461..2031669 (+)	1209	WP_002262650.1	S1C family serine protease	Regulator
SMU_RS09895 (SMU.2165)	spo0J	2032144..2032917 (+)	774	WP_011074697.1	ParB/RepB/Spo0J family partition protein	Regulator

Sequence

Protein

• Length: 402 a.a.
• Molecular weight: 43071.55 Da
• Isoelectric Point: 8.8092

>NTDB_id=381 SMU_RS09890 WP_002262650.1 2030461..2031669(+) (htrA) [Streptococcus mutans UA159]
MNNTKSHPFLKWFIPFLVIFLTFILGVISTLTFNWITGNKSFSNNGKTTVSNVIYDTKSNTTKAVKNVKNTVVSVINYQK
TDNSYYNYDSGSQEKNKSEDGLGVYGEGSGVIYKKDGDSAYLVTNNHVVKDAEKLEIMMANGKKVVGKLVGSDTYSDLAV
IKISSKYVTTVAEFANSDKIKVGEPAIAIGSPLGSDYANSVTEGIVSSLSRTVTSQNENGETISTNAIQTDAAINPGNSG
GALINIKGQVIGINSSKIASSNNSNSGVAVEGMGFAIPSNDVVSIINQLEENGEVVRPALGISMANLSEASTSGRDTLKI
PSDVTSGIVVLSTQSGMPADGKLKKYDVITEIDGKKVASISDLQSILYKHKKGDKIKLTFYREKDKQTVEIQLTKTSQDL
NH

Nucleotide

• Length: 1209 bp

>NTDB_id=381 SMU_RS09890 WP_002262650.1 2030461..2031669(+) (htrA) [Streptococcus mutans UA159]
GTGAATAATACGAAATCTCACCCTTTTTTAAAATGGTTTATACCTTTTTTAGTTATTTTTTTAACTTTTATTTTAGGGGT
CATATCAACACTTACCTTTAATTGGATAACTGGTAACAAATCGTTTTCTAACAATGGAAAAACAACTGTTAGTAATGTCA
TTTATGATACCAAATCTAACACTACCAAGGCTGTTAAAAATGTCAAAAATACAGTTGTATCTGTCATCAATTATCAAAAA
ACAGATAATAGTTATTACAATTATGACAGCGGTTCTCAAGAAAAAAATAAATCAGAGGATGGTCTAGGAGTCTACGGTGA
AGGTTCTGGTGTTATCTATAAAAAAGATGGCGATAGTGCTTATTTAGTTACAAACAATCATGTCGTTAAAGATGCAGAAA
AGTTAGAAATCATGATGGCTAATGGTAAAAAAGTTGTCGGTAAATTAGTAGGTTCTGATACTTATTCTGATCTGGCTGTT
ATTAAAATTTCTTCTAAGTATGTTACGACAGTTGCTGAATTTGCTAATTCGGATAAAATAAAAGTTGGAGAACCAGCAAT
CGCTATTGGTAGCCCTTTAGGCAGTGATTATGCTAATTCTGTAACAGAAGGAATTGTTTCAAGTCTCAGTCGTACAGTAA
CTTCACAAAATGAAAATGGCGAAACAATTTCAACTAATGCTATTCAAACAGACGCAGCTATTAACCCTGGTAATTCTGGT
GGCGCTTTAATTAATATCAAAGGACAAGTTATTGGTATCAATTCAAGTAAAATTGCATCAAGTAATAACTCAAATAGTGG
CGTTGCTGTTGAAGGAATGGGCTTTGCAATTCCTTCAAACGATGTTGTCTCTATTATTAATCAATTAGAAGAAAATGGTG
AAGTTGTTAGACCCGCTCTTGGTATTTCAATGGCTAATCTTAGTGAAGCTTCAACAAGTGGAAGAGATACTTTAAAAATA
CCAAGTGATGTCACAAGCGGTATTGTTGTTCTTTCAACACAAAGTGGTATGCCAGCAGATGGGAAGCTGAAGAAATATGA
TGTCATTACAGAAATTGATGGGAAGAAGGTAGCGTCTATCAGCGATCTTCAAAGTATTCTTTACAAACACAAAAAGGGAG
ATAAAATTAAACTCACTTTCTATCGCGAAAAAGATAAACAAACAGTTGAGATCCAATTAACTAAAACAAGTCAAGATTTG
AATCATTAA

Domains

Predicted by InterproScan.

• PDZ domain (PDZ_2) (298-393)

• Trypsin-like peptidase domain (Trypsin_2) (108-253)

• Peptidase family M50 (Peptidase_M50) (227-398)

3D structure

• AlphaFold DB: Q8DRQ6

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
htrA	Streptococcus gordonii str. Challis substr. CH1	56.14	100	0.564
htrA	Streptococcus mitis NCTC 12261	54.684	100	0.55
htrA	Streptococcus pneumoniae Rx1	54.569	100	0.547
htrA	Streptococcus pneumoniae D39	54.569	100	0.547
htrA	Streptococcus pneumoniae R6	54.569	100	0.547
htrA	Streptococcus pneumoniae TIGR4	54.569	100	0.547

References

• [1]: Kyung-hee Kang et al. (2010) The influence of HtrA expression on the growth of Streptococcus mutans during acid stress. Molecules And Cells 29(3):297-304. [PMID: 20387036]
• [2]: Sang-Joon Ahn et al. (2005) Role of HtrA in growth and competence of Streptococcus mutans UA159. Journal of Bacteriology 187(9):3028-38. [PMID: 15838029]
• [3]: Saswati Biswas et al. (2005) Role of HtrA in surface protein expression and biofilm formation by Streptococcus mutans. Infection And Immunity 73(10):6923-34. [PMID: 16177372]
• [4]: M L Diaz-Torres et al. (2001) HtrA protease and processing of extracellular proteins of Streptococcus mutans. FEMS Microbiology Letters 204(1):23-8. [PMID: 11682172]