Detailed information    

experimental Experimentally validated

Overview

Name   ciaR   Type   Regulator
Locus tag   SMU_RS05200 Genome accession   NC_004350
Coordinates   1070939..1071613 (-) Length   224 a.a.
NCBI ID   WP_002262214.1    Uniprot ID   Q8DU28
Organism   Streptococcus mutans UA159     
Function   repress competence development   
Competence regulation

Function

Deletion of ciaR caused resulted in enhanced transformability in the absence of CSP.


Genomic Context

Location: 1065939..1076613
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SMU_RS05175 (SMU.1124) - 1066246..1067523 (-) 1278 WP_002262219.1 pyrimidine-nucleoside phosphorylase -
  SMU_RS05180 (SMU.1125c) - 1067632..1068228 (-) 597 WP_002262218.1 class I SAM-dependent methyltransferase -
  SMU_RS05185 (SMU.1126) coaA 1068324..1069244 (+) 921 WP_002262217.1 type I pantothenate kinase -
  SMU_RS05190 (SMU.1127) rpsT 1069298..1069552 (+) 255 WP_011074586.1 30S ribosomal protein S20 -
  SMU_RS05195 (SMU.1128) ciaH 1069642..1070949 (-) 1308 WP_002262215.1 three-component system sensor histidine kinase CiaH Regulator
  SMU_RS05200 (SMU.1129) ciaR 1070939..1071613 (-) 675 WP_002262214.1 three-component system response regulator CiaR Regulator
  SMU_RS05205 (SMU.1131c) ciaX 1071664..1071927 (-) 264 WP_002262213.1 three-component system regulator CiaX -
  SMU_RS05210 (SMU.1132) - 1072328..1074877 (-) 2550 WP_002262212.1 M1 family metallopeptidase -
  SMU_RS05215 (SMU.1133) phoU 1074918..1075571 (-) 654 WP_002262211.1 phosphate signaling complex protein PhoU -
  SMU_RS05220 (SMU.1134c) pstB 1075603..1076361 (-) 759 WP_002352282.1 phosphate ABC transporter ATP-binding protein PstB -

Sequence

Protein


Download         Length: 224 a.a.        Molecular weight: 25475.23 Da        Isoelectric Point: 4.3425

>NTDB_id=362 SMU_RS05200 WP_002262214.1 1070939..1071613(-) (ciaR) [Streptococcus mutans UA159]
MIKLLLVEDDLSLSNSIFDFLDDFADVMQVFDGEEGLYEAESGVYDLILLDLMLPEKNGFQVLKELREKGITTPVLIMTA
KEGLDDKGHGFELGADDYLTKPFYLEELKMRIQALLKRSGKFNENNLTYGELTVDTATNTTMVNGKEVELLGKEFDLLVY
FLQNQNVILPKSQIFDRIWGFDSDTTISVVEVYVSKIRKKLKDTTFGKNLQTLRSVGYILKDAN

Nucleotide


Download         Length: 675 bp        

>NTDB_id=362 SMU_RS05200 WP_002262214.1 1070939..1071613(-) (ciaR) [Streptococcus mutans UA159]
ATGATAAAGTTATTATTAGTAGAAGACGATTTGAGTCTATCTAACTCTATCTTTGACTTTCTGGATGATTTTGCTGATGT
CATGCAGGTTTTTGATGGTGAAGAAGGACTGTATGAAGCAGAGAGTGGCGTTTATGATTTGATTCTATTAGACTTAATGC
TTCCTGAAAAAAATGGCTTCCAAGTTTTAAAAGAACTGCGTGAAAAAGGGATAACGACACCTGTTTTGATTATGACAGCT
AAGGAAGGTTTGGATGACAAAGGACACGGTTTTGAATTAGGAGCTGATGATTATCTGACAAAACCATTTTATCTTGAAGA
ATTAAAAATGCGTATTCAGGCCCTCCTCAAACGTTCAGGCAAATTCAACGAAAATAATCTGACTTACGGTGAGTTAACCG
TTGATACAGCAACTAATACAACAATGGTAAATGGAAAAGAAGTTGAACTGCTTGGCAAAGAGTTTGACTTATTAGTTTAT
TTCTTACAAAATCAAAATGTCATTTTACCTAAGTCACAAATTTTTGATCGTATCTGGGGCTTTGATAGCGATACGACAAT
TTCGGTTGTTGAAGTCTATGTTTCTAAAATAAGAAAGAAATTAAAAGACACCACATTTGGAAAGAATTTACAGACATTAC
GCAGTGTAGGATATATTTTGAAGGATGCTAATTAA

Domains

Predicted by InterproScan.

Response regulator receiver domain (Response_reg)

(5-112)

Transcriptional regulatory protein, C terminal (Trans_reg_C)

(145-220)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q8DU28

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ciaR Streptococcus pneumoniae TIGR4

88.393

100

0.884
  ciaR Streptococcus pneumoniae R6

88.393

100

0.884
  ciaR Streptococcus pneumoniae D39

88.393

100

0.884
  ciaR Streptococcus pneumoniae Rx1

88.393

100

0.884
  covR Lactococcus lactis subsp. lactis strain DGCC12653

37.611

100

0.379
  vicR Streptococcus mutans UA159

35.622

100

0.371

Multiple sequence alignment    



References

[1] Chenggang Wu et al. (2010) Regulation of ciaXRH operon expression and identification of the CiaR regulon in Streptococcus mutans. Journal of Bacteriology 192(18):4669-79. [PMID: 20639331]
[2] Xuesong He et al. (2008) The cia operon of Streptococcus mutans encodes a unique component required for calcium-mediated autoregulation. Molecular Microbiology 70(1):112-26. [PMID: 18681938]
[3] Sang-Joon Ahn et al. (2006) Multilevel control of competence development and stress tolerance in Streptococcus mutans UA159. Infection And Immunity 74(3):1631-42. [PMID: 16495534]