Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/-
Location 240867..241561 Replicon chromosome
Accession NC_020518
Organism Escherichia coli str. K-12 substr. MDS42

Toxin (Protein)


Gene name yafO Uniprot ID Q47157
Locus tag ECMDS42_RS01140 Protein ID WP_001263489.1
Coordinates 241163..241561 (+) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID S1QAE3
Locus tag ECMDS42_RS01135 Protein ID WP_000554758.1
Coordinates 240867..241160 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ECMDS42_RS01115 (ECMDS42_0217) 236499..236996 + 498 WP_000006255.1 REP-associated tyrosine transposase RayT -
ECMDS42_RS01120 (ECMDS42_0218) 237220..238959 - 1740 WP_001334802.1 flagellar biosynthesis protein FlhA -
ECMDS42_RS01125 (ECMDS42_0219) 238904..239689 + 786 WP_000207552.1 putative lateral flagellar export/assembly protein LafU -
ECMDS42_RS01130 (ECMDS42_0220) 239760..240815 + 1056 WP_001226164.1 DNA polymerase IV -
ECMDS42_RS01135 (ECMDS42_0221) 240867..241160 + 294 WP_000554758.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
ECMDS42_RS01140 (ECMDS42_0222) 241163..241561 + 399 WP_001263489.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
ECMDS42_RS01145 (ECMDS42_0223) 241571..242023 + 453 WP_001059892.1 GNAT family N-acetyltransferase -
ECMDS42_RS01150 (ECMDS42_0224) 242329..242595 + 267 WP_001295202.1 RtcB family protein -
ECMDS42_RS01155 (ECMDS42_0225) 242528..243064 + 537 WP_001326471.1 peptide chain release factor H -
ECMDS42_RS01160 (ECMDS42_0226) 243121..244578 - 1458 WP_001292994.1 cytosol nonspecific dipeptidase -
ECMDS42_RS01165 (ECMDS42_0227) 244839..245297 + 459 WP_001291990.1 xanthine phosphoribosyltransferase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - gmhA/lpcA 223678..243064 19386


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15487.84 Da        Isoelectric Point: 7.4215

>T28997 WP_001263489.1 NC_020518:241163-241561 [Escherichia coli str. K-12 substr. MDS42]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANENNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

>T28997 NC_020518:241163-241561 [Escherichia coli str. K-12 substr. MDS42]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCCTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGAGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11233.76 Da        Isoelectric Point: 5.2170

>AT28997 WP_000554758.1 NC_020518:240867-241160 [Escherichia coli str. K-12 substr. MDS42]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Download         Length: 294 bp

>AT28997 NC_020518:240867-241160 [Escherichia coli str. K-12 substr. MDS42]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGCGCT
GAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A090J8B1

Antitoxin

Source ID Structure
AlphaFold DB S1QAE3

References