237819

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 127529..128223 Replicon chromosome
Accession NZ_CP093221
Organism Escherichia coli strain ER2683

Toxin (Protein)


Gene name yafO Uniprot ID Q47157
Locus tag MNV48_RS00685 Protein ID WP_001263489.1
Coordinates 127529..127927 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID S1QAE3
Locus tag MNV48_RS00690 Protein ID WP_000554758.1
Coordinates 127930..128223 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
- (123117) 123117..123197 - 81 NuclAT_13 - -
- (123117) 123117..123197 - 81 NuclAT_13 - -
- (123117) 123117..123197 - 81 NuclAT_13 - -
- (123117) 123117..123197 - 81 NuclAT_13 - -
MNV48_RS00660 (123793) 123793..124251 - 459 WP_001291990.1 xanthine phosphoribosyltransferase -
MNV48_RS00665 (124512) 124512..125969 + 1458 WP_001292994.1 cytosol nonspecific dipeptidase -
MNV48_RS00670 (126026) 126026..126547 - 522 Protein_132 peptide chain release factor H -
MNV48_RS00675 (126543) 126543..126749 - 207 Protein_133 RtcB family protein -
MNV48_RS00680 (127067) 127067..127519 - 453 WP_001059892.1 GNAT family N-acetyltransferase -
MNV48_RS00685 (127529) 127529..127927 - 399 WP_001263489.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
MNV48_RS00690 (127930) 127930..128223 - 294 WP_000554758.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
MNV48_RS00695 (128275) 128275..129330 - 1056 WP_001226164.1 DNA polymerase IV -
MNV48_RS00700 (129401) 129401..130186 - 786 WP_000207552.1 putative lateral flagellar export/assembly protein LafU -
MNV48_RS00705 (130158) 130158..131870 + 1713 Protein_139 flagellar biosynthesis protein FlhA -
MNV48_RS00710 (132094) 132094..132591 - 498 WP_000006255.1 REP-associated tyrosine transposase RayT -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island aph(3')-Ia gmhA/lpcA 127529..148066 20537


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15487.84 Da        Isoelectric Point: 7.4215

>T237819 WP_001263489.1 NZ_CP093221:c127927-127529 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANENNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

>T237819 NZ_LR882973:691091-691405 [Escherichia coli]
ATGCACCTGATAACTCAAAAAGCATTGAAAGATGCTGCGGAAAAATACCCGCAACATAAAACGGAGTTGGTGGCTCTGGG
GAACACGATTGCTAAGGGATATTTCAAAAAACCTGAGTCATTAAAAGCAGTATTCCCATCTCTGGATAACTTCAAATATC
TGGATAAGCATTATGTTTTCAATGTTGGGGGCAATGAATTACGTGTTGTAGCAATGGTCTTTTTTGAATCGCAAAAGTGC
TACATACGTGAAGTTATGACGCATAAAGAATACGATTTCTTTACCGTTGTTCATCGTACTAAGGGGAAAAAATGA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11233.76 Da        Isoelectric Point: 5.2170

>AT237819 WP_000554758.1 NZ_CP093221:c128223-127930 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Download         Length: 294 bp

>AT237819 NZ_LR882973:691402-691818 [Escherichia coli]
ATGATTGCGATTGCCGACATCTTGCAAGCAGGAGAAAAGCTAACTGCTGTGGCACCTTTTCTGGCGGGTATTCAGAACGA
GGAACAATACACCCAGGCGCTGGAACTGGTAGATCATCTGCTGCTCAACGATCCTGAAAACCCCTTGCTGGATCTGGTGT
GTGCCAAAATAACCGCGTGGGAAGAATCAGCGCCCGAATTTGCGGAATTTAATGCCATGGCTCAAGCCATGCCTGGCGGT
ATAGCCGTGATTCGTACCCTTATGGATCAATATGGTTTAACCCTTTCCGATCTGCCGGAAATTGGCAGTAAATCTATGGT
GTCACGCGTTTTGAGCGGGAAGAGGAAATTAACGCTGGAACACGCTAAAAAATTGGCAACGCGATTCGGCATTTCTCCCG
CCTTGTTTATTGATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A090J8B1

Antitoxin

Source ID Structure
AlphaFold DB S1QAE3

References