Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/-
Location 234823..235364 Replicon chromosome
Accession NC_020518
Organism Escherichia coli str. K-12 substr. MDS42

Toxin (Protein)


Gene name yafQ Uniprot ID Q47149
Locus tag ECMDS42_RS01100 Protein ID WP_000615983.1
Coordinates 234823..235101 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID Q47150
Locus tag ECMDS42_RS01105 Protein ID WP_000729703.1
Coordinates 235104..235364 (-) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ECMDS42_RS01085 (ECMDS42_0211) 232405..232983 + 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -
ECMDS42_RS01090 (ECMDS42_0212) 233189..233956 + 768 WP_000333380.1 class II glutamine amidotransferase -
ECMDS42_RS01095 (ECMDS42_0213) 233927..234667 - 741 WP_001225679.1 peptidoglycan meso-diaminopimelic acid protein amidase -
ECMDS42_RS01100 (ECMDS42_0214) 234823..235101 - 279 WP_000615983.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
ECMDS42_RS01105 (ECMDS42_0215) 235104..235364 - 261 WP_000729703.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
ECMDS42_RS01110 (ECMDS42_0216) 235574..236323 + 750 WP_000056849.1 C40 family peptidase -
ECMDS42_RS01115 (ECMDS42_0217) 236499..236996 + 498 WP_000006255.1 REP-associated tyrosine transposase RayT -
ECMDS42_RS01120 (ECMDS42_0218) 237220..238959 - 1740 WP_001334802.1 flagellar biosynthesis protein FlhA -
ECMDS42_RS01125 (ECMDS42_0219) 238904..239689 + 786 WP_000207552.1 putative lateral flagellar export/assembly protein LafU -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - gmhA/lpcA 223678..243064 19386


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(4-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-85)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10846.62 Da        Isoelectric Point: 9.9941

>T28996 WP_000615983.1 NC_020518:c235101-234823 [Escherichia coli str. K-12 substr. MDS42]
MIQRDIEYSGQYSKDVKLAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T28996 NC_020518:c235101-234823 [Escherichia coli str. K-12 substr. MDS42]
ATGATTCAAAGGGATATTGAATACTCGGGACAATATTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATACTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9405.80 Da        Isoelectric Point: 5.0758

>AT28996 WP_000729703.1 NC_020518:c235364-235104 [Escherichia coli str. K-12 substr. MDS42]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGIDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT28996 NC_020518:c235364-235104 [Escherichia coli str. K-12 substr. MDS42]
ATGGCTGCTAACGCGTTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCAGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCATTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4Q2U

Antitoxin

Source ID Structure
PDB 4Q2U
AlphaFold DB A0A5F1F716

References