Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 4097061..4097755 Replicon chromosome
Accession NZ_CP116994
Organism Escherichia coli strain MLI108K1

Toxin (Protein)


Gene name yafO Uniprot ID Q47157
Locus tag NL409_RS21815 Protein ID WP_001263489.1
Coordinates 4097357..4097755 (+) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID S1QAE3
Locus tag NL409_RS21810 Protein ID WP_000554758.1
Coordinates 4097061..4097354 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NL409_RS21790 (4092693) 4092693..4093190 + 498 WP_000006255.1 REP-associated tyrosine transposase RayT -
NL409_RS21795 (4093414) 4093414..4095126 - 1713 Protein_3906 flagellar biosynthesis protein FlhA -
NL409_RS21800 (4095098) 4095098..4095883 + 786 WP_000207552.1 putative lateral flagellar export/assembly protein LafU -
NL409_RS21805 (4095954) 4095954..4097009 + 1056 WP_001226164.1 DNA polymerase IV -
NL409_RS21810 (4097061) 4097061..4097354 + 294 WP_000554758.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
NL409_RS21815 (4097357) 4097357..4097755 + 399 WP_001263489.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
NL409_RS21820 (4097765) 4097765..4098217 + 453 WP_001059892.1 GNAT family N-acetyltransferase -
NL409_RS21825 (4098535) 4098535..4098741 + 207 Protein_3912 RtcB family protein -
NL409_RS21830 (4098737) 4098737..4099258 + 522 Protein_3913 peptide chain release factor H -
NL409_RS21835 (4099315) 4099315..4100772 - 1458 WP_001292994.1 cytosol nonspecific dipeptidase -
NL409_RS21840 (4101033) 4101033..4101491 + 459 WP_001291990.1 xanthine phosphoribosyltransferase -
- (4102087) 4102087..4102167 + 81 NuclAT_11 - -
- (4102087) 4102087..4102167 + 81 NuclAT_11 - -
- (4102087) 4102087..4102167 + 81 NuclAT_11 - -
- (4102087) 4102087..4102167 + 81 NuclAT_11 - -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - gmhA/lpcA 4083298..4099258 15960


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15487.84 Da        Isoelectric Point: 7.4215

>T269695 WP_001263489.1 NZ_CP116994:4097357-4097755 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANENNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11233.76 Da        Isoelectric Point: 5.2170

>AT269695 WP_000554758.1 NZ_CP116994:4097061-4097354 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Download         Length: 294 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A090J8B1

Antitoxin

Source ID Structure
AlphaFold DB S1QAE3

References