Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 2495439..2495980 Replicon chromosome
Accession NZ_CP079767
Organism Escherichia coli strain SF10

Toxin (Protein)


Gene name yafQ Uniprot ID Q47149
Locus tag KX927_RS14640 Protein ID WP_000615983.1
Coordinates 2495702..2495980 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID Q47150
Locus tag KX927_RS14635 Protein ID WP_000729703.1
Coordinates 2495439..2495699 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KX927_RS14615 (2491114) 2491114..2491899 - 786 WP_000207552.1 putative lateral flagellar export/assembly protein LafU -
KX927_RS14620 (2491871) 2491871..2493583 + 1713 Protein_2459 flagellar biosynthesis protein FlhA -
KX927_RS14625 (2493807) 2493807..2494304 - 498 WP_000006255.1 REP-associated tyrosine transposase RayT -
KX927_RS14630 (2494480) 2494480..2495229 - 750 WP_000056849.1 C40 family peptidase -
KX927_RS14635 (2495439) 2495439..2495699 + 261 WP_000729703.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
KX927_RS14640 (2495702) 2495702..2495980 + 279 WP_000615983.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
KX927_RS14645 (2496136) 2496136..2496876 + 741 WP_001225679.1 peptidoglycan meso-diaminopimelic acid protein amidase -
KX927_RS14650 (2496847) 2496847..2497614 - 768 WP_000333380.1 class II glutamine amidotransferase -
KX927_RS14655 (2497820) 2497820..2498398 - 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - gmhA/lpcA 2489242..2507914 18672


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(4-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-85)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10846.62 Da        Isoelectric Point: 9.9941

>T209980 WP_000615983.1 NZ_CP079767:2495702-2495980 [Escherichia coli]
MIQRDIEYSGQYSKDVKLAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T209980 NZ_CP079767:2495702-2495980 [Escherichia coli]
ATGATTCAAAGGGATATTGAATACTCGGGACAATATTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATACTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9405.80 Da        Isoelectric Point: 5.0758

>AT209980 WP_000729703.1 NZ_CP079767:2495439-2495699 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGIDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT209980 NZ_CP079767:2495439-2495699 [Escherichia coli]
ATGGCTGCTAACGCGTTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCAGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCATTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4Q2U

Antitoxin

Source ID Structure
PDB 4Q2U
AlphaFold DB A0A5F1F716

References