Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 1086353..1087047 Replicon chromosome
Accession NZ_CP034589
Organism Escherichia coli strain L37

Toxin (Protein)


Gene name yafO Uniprot ID S1EXB8
Locus tag EKM58_RS05230 Protein ID WP_001263493.1
Coordinates 1086649..1087047 (+) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID S1FJN6
Locus tag EKM58_RS05225 Protein ID WP_000554757.1
Coordinates 1086353..1086646 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EKM58_RS05205 1081985..1082482 + 498 WP_000006255.1 REP-associated tyrosine transposase RayT -
EKM58_RS05210 1082706..1084418 - 1713 Protein_987 flagellar biosynthesis protein FlhA -
EKM58_RS05215 1084390..1085175 + 786 WP_000207564.1 putative lateral flagellar export/assembly protein LafU -
EKM58_RS05220 1085246..1086301 + 1056 WP_063501914.1 DNA polymerase IV -
EKM58_RS05225 1086353..1086646 + 294 WP_000554757.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
EKM58_RS05230 1086649..1087047 + 399 WP_001263493.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
EKM58_RS05235 1087057..1087509 + 453 WP_001059847.1 GNAT family N-acetyltransferase -
EKM58_RS05240 1087755..1087958 + 204 Protein_993 RNA ligase RtcB family protein -
EKM58_RS05245 1087957..1088478 + 522 Protein_994 peptide chain release factor H -
EKM58_RS05250 1088535..1089992 - 1458 WP_001293003.1 cytosol nonspecific dipeptidase -
EKM58_RS05255 1090253..1090711 + 459 WP_001291990.1 xanthine phosphoribosyltransferase -
EKM58_RS05260 1090803..1092047 + 1245 WP_000189541.1 esterase FrsA -
- 1091307..1091387 + 81 NuclAT_11 - -
- 1091307..1091387 + 81 NuclAT_11 - -
- 1091307..1091387 + 81 NuclAT_11 - -
- 1091307..1091387 + 81 NuclAT_11 - -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - gmhA/lpcA 1068707..1088478 19771


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15472.83 Da        Isoelectric Point: 8.0949

>T116233 WP_001263493.1 NZ_CP034589:1086649-1087047 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKQARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

>T116233 NZ_CP034589:1086649-1087047 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACAGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11233.81 Da        Isoelectric Point: 5.9050

>AT116233 WP_000554757.1 NZ_CP034589:1086353-1086646 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Download         Length: 294 bp

>AT116233 NZ_CP034589:1086353-1086646 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATTACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGAGCT
GAAAAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1EXB8

Antitoxin

Source ID Structure
AlphaFold DB S1FJN6

References