Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 2489242..2489936 Replicon chromosome
Accession NZ_CP079767
Organism Escherichia coli strain SF10

Toxin (Protein)


Gene name yafO Uniprot ID Q47157
Locus tag KX927_RS14600 Protein ID WP_001263489.1
Coordinates 2489242..2489640 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID S1QAE3
Locus tag KX927_RS14605 Protein ID WP_000554758.1
Coordinates 2489643..2489936 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
- (2484830) 2484830..2484910 - 81 NuclAT_12 - -
- (2484830) 2484830..2484910 - 81 NuclAT_12 - -
- (2484830) 2484830..2484910 - 81 NuclAT_12 - -
- (2484830) 2484830..2484910 - 81 NuclAT_12 - -
KX927_RS14575 (2485506) 2485506..2485964 - 459 WP_001291990.1 xanthine phosphoribosyltransferase -
KX927_RS14580 (2486225) 2486225..2487682 + 1458 WP_001292994.1 cytosol nonspecific dipeptidase -
KX927_RS14585 (2487739) 2487739..2488260 - 522 Protein_2452 peptide chain release factor H -
KX927_RS14590 (2488256) 2488256..2488462 - 207 Protein_2453 RtcB family protein -
KX927_RS14595 (2488780) 2488780..2489232 - 453 WP_001059892.1 GNAT family N-acetyltransferase -
KX927_RS14600 (2489242) 2489242..2489640 - 399 WP_001263489.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
KX927_RS14605 (2489643) 2489643..2489936 - 294 WP_000554758.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
KX927_RS14610 (2489988) 2489988..2491043 - 1056 WP_001226164.1 DNA polymerase IV -
KX927_RS14615 (2491114) 2491114..2491899 - 786 WP_000207552.1 putative lateral flagellar export/assembly protein LafU -
KX927_RS14620 (2491871) 2491871..2493583 + 1713 Protein_2459 flagellar biosynthesis protein FlhA -
KX927_RS14625 (2493807) 2493807..2494304 - 498 WP_000006255.1 REP-associated tyrosine transposase RayT -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - gmhA/lpcA 2489242..2507914 18672


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15487.84 Da        Isoelectric Point: 7.4215

>T209979 WP_001263489.1 NZ_CP079767:c2489640-2489242 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANENNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

>T209979 NZ_CP104358:c989052-988525 [Salmonella enterica]
ATGATGTTTACAGACTGGCATGAGGCCGCGATAGGGAAAACCCACAATCGAATGAATTTTGATTGTGGGGATGCCGATCT
GAACCAATTCCTGCAACGCCATGCACGACAAAATCATGAGAAAGGGACAACGAAAACCTATGTTGCGCTTGATAATTCGG
ATGTTACACGTATCCACGGCTTTTACTCAGTCAGTCCTGCATCACTGATATATGCACAGGTTCCCGGCGCAATCAGCAAA
GGATTAGGGAGATACGATGTGCCAGTGTTTCGTCTGGGTCGTTTAGCCGTAGACAAATCTATGCAGGGGCAAGGGCTGGG
AGCACAACTTTTGTTATCTGCCGGAAAGCGTTGCATACAGGCGGCTTTGCAGGTCGGTGGCGTAGCCCTACTTATTGATG
CCAAAAATAAACAGGTCTGCGACTGGTACAAAGGATTTGGCGCAGTACCATTAAACGATCAACCCCTTTCCTTGTTGCTG
TCGTTTAAAACGCTTTATGCTGCTTTATCTGCATCTGGTAGGTTATGA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11233.76 Da        Isoelectric Point: 5.2170

>AT209979 WP_000554758.1 NZ_CP079767:c2489936-2489643 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Download         Length: 294 bp

>AT209979 NZ_CP104358:c989339-989049 [Salmonella enterica]
ATGAAAACCATGCCTCAGATAGCTATAGAATCTAACGAAAGGCTGTCGTTGCGTGTATCGACGGATGCCAAAAAGTTGAT
AGTCCGCGCTGCGGCAATCCAGCAAACCAACCTGACGGATTTTGTGGTCAGTAACGTACTACCTGTCGCACAGAAGATTG
TTGATGCCGCCGAACGGGTATATCTGACGGAGCGCGATACTCAAATGATCATGGAAATTTTAGATAACCCGCCAGCGCCG
AATGAAAAGCTTCTTGCCGCTGCTTTCGCCCTGCCGGACATGAAGAAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A090J8B1

Antitoxin

Source ID Structure
AlphaFold DB S1QAE3

References