Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 245963..246504 Replicon chromosome
Accession NZ_CP034658
Organism Escherichia coli strain ATCC 98082

Toxin (Protein)


Gene name yafQ Uniprot ID Q47149
Locus tag EKH78_RS01140 Protein ID WP_000615983.1
Coordinates 245963..246241 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID Q47150
Locus tag EKH78_RS01145 Protein ID WP_000729703.1
Coordinates 246244..246504 (-) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EKH78_RS01125 (243545) 243545..244123 + 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -
EKH78_RS01130 (244329) 244329..245096 + 768 WP_000333380.1 class II glutamine amidotransferase -
EKH78_RS01135 (245067) 245067..245807 - 741 WP_001225679.1 peptidoglycan meso-diaminopimelic acid protein amidase -
EKH78_RS01140 (245963) 245963..246241 - 279 WP_000615983.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
EKH78_RS01145 (246244) 246244..246504 - 261 WP_000729703.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
EKH78_RS01150 (246714) 246714..247463 + 750 WP_000056849.1 C40 family peptidase -
EKH78_RS01155 (247639) 247639..248136 + 498 WP_000006255.1 REP-associated tyrosine transposase RayT -
EKH78_RS01160 (248360) 248360..250072 - 1713 Protein_226 flagellar biosynthesis protein FlhA -
EKH78_RS01165 (250044) 250044..250829 + 786 WP_000207552.1 putative lateral flagellar export/assembly protein LafU -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - gmhA/lpcA 234818..254204 19386


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(4-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-85)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10846.62 Da        Isoelectric Point: 9.9941

>T116299 WP_000615983.1 NZ_CP034658:c246241-245963 [Escherichia coli]
MIQRDIEYSGQYSKDVKLAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T116299 NZ_CP034658:c246241-245963 [Escherichia coli]
ATGATTCAAAGGGATATTGAATACTCGGGACAATATTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATACTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9405.80 Da        Isoelectric Point: 5.0758

>AT116299 WP_000729703.1 NZ_CP034658:c246504-246244 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGIDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT116299 NZ_CP034658:c246504-246244 [Escherichia coli]
ATGGCTGCTAACGCGTTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCAGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCATTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4Q2U

Antitoxin

Source ID Structure
PDB 4Q2U
AlphaFold DB A0A5F1F716

References