Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 1005049..1005743 Replicon chromosome
Accession NZ_CP013952
Organism Escherichia coli strain HST04

Toxin (Protein)


Gene name yafO Uniprot ID Q47157
Locus tag AWH44_RS05365 Protein ID WP_001263489.1
Coordinates 1005049..1005447 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID S1QAE3
Locus tag AWH44_RS05370 Protein ID WP_000554758.1
Coordinates 1005450..1005743 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AWH44_RS05330 1000098..1000907 - 810 WP_000160710.1 2-keto-4-pentenoate hydratase -
AWH44_RS05335 1000917..1001648 - 732 Protein_961 2-hydroxy-6-oxonona-2,4-dienedioate hydrolase -
AWH44_RS24160 1001640..1001771 - 132 Protein_962 xanthine phosphoribosyltransferase -
AWH44_RS05340 1002032..1003489 + 1458 WP_001292994.1 cytosol nonspecific dipeptidase -
AWH44_RS05345 1003546..1004067 - 522 Protein_964 peptide chain release factor H -
AWH44_RS05350 1004063..1004269 - 207 Protein_965 RtcB family protein -
AWH44_RS05360 1004587..1005039 - 453 WP_001059892.1 GNAT family N-acetyltransferase -
AWH44_RS05365 1005049..1005447 - 399 WP_001263489.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
AWH44_RS05370 1005450..1005743 - 294 WP_000554758.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
AWH44_RS05375 1005795..1006850 - 1056 WP_001226164.1 DNA polymerase IV -
AWH44_RS05380 1006921..1007706 - 786 WP_000207552.1 putative lateral flagellar export/assembly protein LafU -
AWH44_RS05385 1007678..1009390 + 1713 Protein_971 flagellar biosynthesis protein FlhA -
AWH44_RS05390 1009614..1010111 - 498 WP_000006255.1 REP-associated tyrosine transposase RayT -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - gmhA/lpcA 1005049..1023721 18672


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15487.84 Da        Isoelectric Point: 7.4215

>T59649 WP_001263489.1 NZ_CP013952:c1005447-1005049 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANENNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

>T59649 NZ_CP013952:c1005447-1005049 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCCTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGAGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11233.76 Da        Isoelectric Point: 5.2170

>AT59649 WP_000554758.1 NZ_CP013952:c1005743-1005450 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Download         Length: 294 bp

>AT59649 NZ_CP013952:c1005743-1005450 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGCGCT
GAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A090J8B1

Antitoxin

Source ID Structure
AlphaFold DB S1QAE3

References