Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 4279585..4280126 Replicon chromosome
Accession NZ_CP080620
Organism Escherichia coli strain TOP10

Toxin (Protein)


Gene name yafQ Uniprot ID Q47149
Locus tag K3F56_RS20535 Protein ID WP_000615983.1
Coordinates 4279585..4279863 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID Q47150
Locus tag K3F56_RS20540 Protein ID WP_000729703.1
Coordinates 4279866..4280126 (-) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K3F56_RS20520 (4277167) 4277167..4277745 + 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -
K3F56_RS20525 (4277951) 4277951..4278718 + 768 WP_000333380.1 class II glutamine amidotransferase -
K3F56_RS20530 (4278689) 4278689..4279429 - 741 WP_001225679.1 peptidoglycan meso-diaminopimelic acid protein amidase -
K3F56_RS20535 (4279585) 4279585..4279863 - 279 WP_000615983.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
K3F56_RS20540 (4279866) 4279866..4280126 - 261 WP_000729703.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
K3F56_RS20545 (4280336) 4280336..4281085 + 750 WP_000056849.1 C40 family peptidase -
K3F56_RS20550 (4281261) 4281261..4281758 + 498 WP_000006255.1 REP-associated tyrosine transposase RayT -
K3F56_RS20555 (4281982) 4281982..4283694 - 1713 Protein_4045 flagellar biosynthesis protein FlhA -
K3F56_RS20560 (4283666) 4283666..4284451 + 786 WP_000207552.1 putative lateral flagellar export/assembly protein LafU -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - gmhA/lpcA 4267651..4287826 20175


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(4-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-85)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10846.62 Da        Isoelectric Point: 9.9941

>T212292 WP_000615983.1 NZ_CP080620:c4279863-4279585 [Escherichia coli]
MIQRDIEYSGQYSKDVKLAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T212292 NZ_CP107001:2247036-2247323 [Escherichia coli]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9405.80 Da        Isoelectric Point: 5.0758

>AT212292 WP_000729703.1 NZ_CP080620:c4280126-4279866 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGIDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT212292 NZ_CP107001:2246797-2247036 [Escherichia coli]
ATGGGTAGCATTAACCTGCGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGTGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGGCTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4Q2U

Antitoxin

Source ID Structure
PDB 4Q2U
AlphaFold DB A0A5F1F716

References