Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 1946010..1946704 Replicon chromosome
Accession NZ_CP026612
Organism Escherichia coli strain DTU-1

Toxin (Protein)


Gene name yafO Uniprot ID Q47157
Locus tag C4J63_RS09905 Protein ID WP_001263489.1
Coordinates 1946306..1946704 (+) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID S1QAE3
Locus tag C4J63_RS09900 Protein ID WP_000554758.1
Coordinates 1946010..1946303 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C4J63_RS09880 1941642..1942139 + 498 WP_000006255.1 REP-associated tyrosine transposase RayT -
C4J63_RS09885 1942363..1944075 - 1713 Protein_1788 flagellar biosynthesis protein FlhA -
C4J63_RS09890 1944047..1944832 + 786 WP_000207552.1 putative lateral flagellar export/assembly protein LafU -
C4J63_RS09895 1944903..1945958 + 1056 WP_001226164.1 DNA polymerase IV -
C4J63_RS09900 1946010..1946303 + 294 WP_000554758.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
C4J63_RS09905 1946306..1946704 + 399 WP_001263489.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
C4J63_RS09910 1946714..1947166 + 453 WP_001059892.1 GNAT family N-acetyltransferase -
C4J63_RS09915 1947484..1947690 + 207 Protein_1794 RtcB family protein -
C4J63_RS09920 1947686..1948207 + 522 Protein_1795 peptide chain release factor H -
C4J63_RS09925 1948264..1949721 - 1458 WP_001292994.1 cytosol nonspecific dipeptidase -
C4J63_RS23140 1949982..1950113 + 132 Protein_1797 xanthine phosphoribosyltransferase -
C4J63_RS09930 1950105..1950836 + 732 Protein_1798 2-hydroxy-6-oxonona-2,4-dienedioate hydrolase -
C4J63_RS09935 1950846..1951655 + 810 WP_000160710.1 2-keto-4-pentenoate hydratase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - gmhA/lpcA 1928821..1948207 19386


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15487.84 Da        Isoelectric Point: 7.4215

>T95271 WP_001263489.1 NZ_CP026612:1946306-1946704 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANENNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

>T95271 NZ_CP026612:1946306-1946704 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCCTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGAGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11233.76 Da        Isoelectric Point: 5.2170

>AT95271 WP_000554758.1 NZ_CP026612:1946010-1946303 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Download         Length: 294 bp

>AT95271 NZ_CP026612:1946010-1946303 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGCGCT
GAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A090J8B1

Antitoxin

Source ID Structure
AlphaFold DB S1QAE3

References