Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 918499..919193 Replicon chromosome
Accession NZ_CP063487
Organism Escherichia coli strain EF7-18-51

Toxin (Protein)


Gene name yafO Uniprot ID S1EXB8
Locus tag IQS15_RS04450 Protein ID WP_001263493.1
Coordinates 918499..918897 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID S1FJN6
Locus tag IQS15_RS04455 Protein ID WP_000554757.1
Coordinates 918900..919193 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IQS15_RS04420 913499..914743 - 1245 WP_000189541.1 esterase FrsA -
- 914159..914239 - 81 NuclAT_12 - -
- 914159..914239 - 81 NuclAT_12 - -
- 914159..914239 - 81 NuclAT_12 - -
- 914159..914239 - 81 NuclAT_12 - -
IQS15_RS04425 914835..915293 - 459 WP_001291990.1 xanthine phosphoribosyltransferase -
IQS15_RS04430 915554..917011 + 1458 WP_001293003.1 cytosol nonspecific dipeptidase -
IQS15_RS04435 917068..917589 - 522 Protein_865 peptide chain release factor H -
IQS15_RS04440 917588..917791 - 204 Protein_866 RNA ligase RtcB family protein -
IQS15_RS04445 918037..918489 - 453 WP_001059847.1 GNAT family N-acetyltransferase -
IQS15_RS04450 918499..918897 - 399 WP_001263493.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
IQS15_RS04455 918900..919193 - 294 WP_000554757.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
IQS15_RS04460 919245..920300 - 1056 WP_001226164.1 DNA polymerase IV -
IQS15_RS04465 920371..921156 - 786 WP_000207564.1 putative lateral flagellar export/assembly protein LafU -
IQS15_RS04470 921128..922840 + 1713 Protein_872 flagellar biosynthesis protein FlhA -
IQS15_RS04475 923064..923561 - 498 WP_000006255.1 REP-associated tyrosine transposase RayT -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - gmhA/lpcA 917537..938816 21279


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15472.83 Da        Isoelectric Point: 8.0949

>T178443 WP_001263493.1 NZ_CP063487:c918897-918499 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKQARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

>T178443 NZ_CP084026:699917-700324 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCACTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCTGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11233.81 Da        Isoelectric Point: 5.9050

>AT178443 WP_000554757.1 NZ_CP063487:c919193-918900 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Download         Length: 294 bp

>AT178443 NZ_CP084026:699670-699936 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1EXB8

Antitoxin

Source ID Structure
AlphaFold DB S1FJN6

References