Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 252007..252701 Replicon chromosome
Accession NZ_CP034658
Organism Escherichia coli strain ATCC 98082

Toxin (Protein)


Gene name yafO Uniprot ID Q47157
Locus tag EKH78_RS01180 Protein ID WP_001263489.1
Coordinates 252303..252701 (+) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID S1QAE3
Locus tag EKH78_RS01175 Protein ID WP_000554758.1
Coordinates 252007..252300 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EKH78_RS01155 (247639) 247639..248136 + 498 WP_000006255.1 REP-associated tyrosine transposase RayT -
EKH78_RS01160 (248360) 248360..250072 - 1713 Protein_226 flagellar biosynthesis protein FlhA -
EKH78_RS01165 (250044) 250044..250829 + 786 WP_000207552.1 putative lateral flagellar export/assembly protein LafU -
EKH78_RS01170 (250900) 250900..251955 + 1056 WP_001226164.1 DNA polymerase IV -
EKH78_RS01175 (252007) 252007..252300 + 294 WP_000554758.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
EKH78_RS01180 (252303) 252303..252701 + 399 WP_001263489.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
EKH78_RS01185 (252711) 252711..253163 + 453 WP_001059892.1 GNAT family N-acetyltransferase -
EKH78_RS01190 (253481) 253481..253687 + 207 Protein_232 RtcB family protein -
EKH78_RS01195 (253683) 253683..254204 + 522 Protein_233 peptide chain release factor H -
EKH78_RS01200 (254261) 254261..255718 - 1458 WP_001292994.1 cytosol nonspecific dipeptidase -
EKH78_RS01205 (255979) 255979..256437 + 459 WP_001291990.1 xanthine phosphoribosyltransferase -
- (257033) 257033..257113 + 81 NuclAT_12 - -
- (257033) 257033..257113 + 81 NuclAT_12 - -
- (257033) 257033..257113 + 81 NuclAT_12 - -
- (257033) 257033..257113 + 81 NuclAT_12 - -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - gmhA/lpcA 234818..254204 19386


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15487.84 Da        Isoelectric Point: 7.4215

>T116300 WP_001263489.1 NZ_CP034658:252303-252701 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANENNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

>T116300 NZ_CP034658:252303-252701 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCCTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGAGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11233.76 Da        Isoelectric Point: 5.2170

>AT116300 WP_000554758.1 NZ_CP034658:252007-252300 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Download         Length: 294 bp

>AT116300 NZ_CP034658:252007-252300 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGCGCT
GAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A090J8B1

Antitoxin

Source ID Structure
AlphaFold DB S1QAE3

References