Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 253326..254020 Replicon chromosome
Accession NZ_CP053605
Organism Escherichia coli strain NEB_Turbo

Toxin (Protein)


Gene name yafO Uniprot ID Q47157
Locus tag HO393_RS01180 Protein ID WP_001263489.1
Coordinates 253622..254020 (+) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID S1QAE3
Locus tag HO393_RS01175 Protein ID WP_000554758.1
Coordinates 253326..253619 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HO393_RS01155 248958..249455 + 498 WP_000006255.1 REP-associated tyrosine transposase RayT -
HO393_RS01160 249679..251418 - 1740 WP_001334802.1 flagellar biosynthesis protein FlhA -
HO393_RS01165 251363..252148 + 786 WP_000207552.1 putative lateral flagellar export/assembly protein LafU -
HO393_RS01170 252219..253274 + 1056 WP_001226164.1 DNA polymerase IV -
HO393_RS01175 253326..253619 + 294 WP_000554758.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
HO393_RS01180 253622..254020 + 399 WP_001263489.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
HO393_RS01185 254030..254482 + 453 WP_001059892.1 GNAT family N-acetyltransferase -
HO393_RS01190 254788..255054 + 267 WP_001295202.1 hypothetical protein -
HO393_RS01195 254966..255523 + 558 WP_001352051.1 peptide chain release factor H -
HO393_RS01200 255580..257037 - 1458 WP_001292994.1 cytosol nonspecific dipeptidase -
HO393_RS01205 257421..258152 + 732 Protein_233 2-hydroxy-6-oxonona-2,4-dienedioate hydrolase -
HO393_RS01210 258162..258971 + 810 WP_000160710.1 2-keto-4-pentenoate hydratase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - gmhA/lpcA 236137..255523 19386


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15487.84 Da        Isoelectric Point: 7.4215

>T157124 WP_001263489.1 NZ_CP053605:253622-254020 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANENNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

>T157124 NZ_CP069076:3172423-3172866 [Mycobacterium tuberculosis]
ATGCTGTGCGTTGATGTCAACGTGCTCGTTTACGCGCATCGGGCAGACCTACGGGAGCACGCGGACTATCGGGGTTTGCT
TGAGCGGCTGGCCAACGATGACGAGCCGCTGGGTCTACCAGATAGCGTGCTCGCCGGCTTCATCCGGGTGGTTACCAACC
GCCGCGTCTTCACCGAGCCGACGAGCCCACAGGACGCATGGCAGGCAGTCGACGCCCTACTCGCGGCACCCGCAGCCATG
CGACTTCGGCCTGGCGAGCGCCACTGGATGGCCTTTCGGCAGTTAGCGTCCGATGTTGATGCGAACGGCAACGACATTGC
GGACGCGCACCTGGCCGCCTACGCGCTAGAGAACAACGCAACCTGGTTGAGCGCCGACCGCGGCTTTGCCCGTTTCCGTC
GACTGCGCTGGCGTCATCCGTTGGACGGTCAGACCCATCTATAA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11233.76 Da        Isoelectric Point: 5.2170

>AT157124 WP_000554758.1 NZ_CP053605:253326-253619 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Download         Length: 294 bp

>AT157124 NZ_CP069076:3172179-3172436 [Mycobacterium tuberculosis]
GTGCGCACGACGATCCGTATCGATGACGAGCTGTACCGCGAGGTGAAAGCAAAGGCCGCTCGTTCCGGGCGTACCGTGGC
CGCGGTTCTTGAAGATGCGGTGCGGCGTGGTCTCAACCCGCCTAAGCCGCAGGCCGCCGGCCGTTATCGAGTCCAGCCGT
CGGGTAAGGGCGGCCTGCGGCCCGGTGTCGATCTATCGTCCAACGCCGCACTTGCCGAAGCGATGAACGACGGCGTGTCG
GTCGATGCTGTGCGTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A090J8B1

Antitoxin

Source ID Structure
AlphaFold DB S1QAE3

References