Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 1137974..1138515 Replicon chromosome
Accession NZ_CP079992
Organism Escherichia coli strain KMS2001

Toxin (Protein)


Gene name yafQ Uniprot ID Q47149
Locus tag KYK85_RS05440 Protein ID WP_000615983.1
Coordinates 1137974..1138252 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID Q47150
Locus tag KYK85_RS05445 Protein ID WP_000729703.1
Coordinates 1138255..1138515 (-) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KYK85_RS05425 (1135556) 1135556..1136134 + 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -
KYK85_RS05430 (1136340) 1136340..1137107 + 768 WP_000333380.1 class II glutamine amidotransferase -
KYK85_RS05435 (1137078) 1137078..1137818 - 741 WP_001225679.1 peptidoglycan meso-diaminopimelic acid protein amidase -
KYK85_RS05440 (1137974) 1137974..1138252 - 279 WP_000615983.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
KYK85_RS05445 (1138255) 1138255..1138515 - 261 WP_000729703.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
KYK85_RS05450 (1138725) 1138725..1139474 + 750 WP_000056849.1 C40 family peptidase -
KYK85_RS05455 (1139650) 1139650..1140147 + 498 WP_000006255.1 REP-associated tyrosine transposase RayT -
KYK85_RS05460 (1140371) 1140371..1142083 - 1713 Protein_1073 flagellar biosynthesis protein FlhA -
KYK85_RS05465 (1142055) 1142055..1142840 + 786 WP_000207552.1 putative lateral flagellar export/assembly protein LafU -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - gmhA/lpcA 1126040..1146215 20175


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(4-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-85)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10846.62 Da        Isoelectric Point: 9.9941

>T210489 WP_000615983.1 NZ_CP079992:c1138252-1137974 [Escherichia coli]
MIQRDIEYSGQYSKDVKLAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T210489 NZ_CP104526:3535312-3535530 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9405.80 Da        Isoelectric Point: 5.0758

>AT210489 WP_000729703.1 NZ_CP079992:c1138515-1138255 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGIDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT210489 NZ_CP104526:3534912-3535286 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTAAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4Q2U

Antitoxin

Source ID Structure
PDB 4Q2U
AlphaFold DB A0A5F1F716

References