Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 3635024..3635565 Replicon chromosome
Accession NZ_CP032667
Organism Escherichia coli str. K-12 substr. MG1655 strain K-12

Toxin (Protein)


Gene name yafQ Uniprot ID Q47149
Locus tag D8B36_RS18085 Protein ID WP_000615983.1
Coordinates 3635287..3635565 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID Q47150
Locus tag D8B36_RS18080 Protein ID WP_000729703.1
Coordinates 3635024..3635284 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
D8B36_RS18060 3630699..3631484 - 786 WP_000207552.1 putative lateral flagellar export/assembly protein LafU -
D8B36_RS18065 3631456..3633168 + 1713 Protein_3465 flagellar biosynthesis protein FlhA -
D8B36_RS18070 3633392..3633889 - 498 WP_000006255.1 REP-associated tyrosine transposase RayT -
D8B36_RS18075 3634065..3634814 - 750 WP_000056849.1 C40 family peptidase -
D8B36_RS18080 3635024..3635284 + 261 WP_000729703.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
D8B36_RS18085 3635287..3635565 + 279 WP_000615983.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
D8B36_RS18090 3635721..3636461 + 741 WP_001225679.1 murein L,D-transpeptidase -
D8B36_RS18095 3636432..3637199 - 768 WP_000333380.1 class II glutamine amidotransferase -
D8B36_RS18100 3637405..3637983 - 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - gmhA/lpcA 3628827..3647499 18672


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(4-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-85)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10846.62 Da        Isoelectric Point: 9.9941

>T112081 WP_000615983.1 NZ_CP032667:3635287-3635565 [Escherichia coli str. K-12 substr. MG1655]
MIQRDIEYSGQYSKDVKLAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T112081 NZ_CP032667:3635287-3635565 [Escherichia coli str. K-12 substr. MG1655]
ATGATTCAAAGGGATATTGAATACTCGGGACAATATTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATACTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9405.80 Da        Isoelectric Point: 5.0758

>AT112081 WP_000729703.1 NZ_CP032667:3635024-3635284 [Escherichia coli str. K-12 substr. MG1655]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGIDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT112081 NZ_CP032667:3635024-3635284 [Escherichia coli str. K-12 substr. MG1655]
ATGGCTGCTAACGCGTTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCAGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCATTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4Q2U

Antitoxin

Source ID Structure
PDB 4Q2U
AlphaFold DB A0A5F1F716

References