Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 2500627..2501168 Replicon chromosome
Accession NZ_CP079770
Organism Escherichia coli strain HL15

Toxin (Protein)


Gene name yafQ Uniprot ID Q47149
Locus tag KYX58_RS13980 Protein ID WP_000615983.1
Coordinates 2500890..2501168 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID Q47150
Locus tag KYX58_RS13975 Protein ID WP_000729703.1
Coordinates 2500627..2500887 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KYX58_RS13955 (2496302) 2496302..2497087 - 786 WP_000207552.1 putative lateral flagellar export/assembly protein LafU -
KYX58_RS13960 (2497059) 2497059..2498771 + 1713 Protein_2457 flagellar biosynthesis protein FlhA -
KYX58_RS13965 (2498995) 2498995..2499492 - 498 WP_000006255.1 REP-associated tyrosine transposase RayT -
KYX58_RS13970 (2499668) 2499668..2500417 - 750 WP_000056849.1 C40 family peptidase -
KYX58_RS13975 (2500627) 2500627..2500887 + 261 WP_000729703.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
KYX58_RS13980 (2500890) 2500890..2501168 + 279 WP_000615983.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
KYX58_RS13985 (2501324) 2501324..2502064 + 741 WP_001225679.1 peptidoglycan meso-diaminopimelic acid protein amidase -
KYX58_RS13990 (2502035) 2502035..2502802 - 768 WP_000333380.1 class II glutamine amidotransferase -
KYX58_RS13995 (2503008) 2503008..2503586 - 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - gmhA/lpcA 2494430..2513102 18672


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(4-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-85)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10846.62 Da        Isoelectric Point: 9.9941

>T210005 WP_000615983.1 NZ_CP079770:2500890-2501168 [Escherichia coli]
MIQRDIEYSGQYSKDVKLAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T210005 NZ_CP104362:c4495231-4494920 [Salmonella enterica]
ATGCAATTTATAGAAACGGAACTCTTTACTGAAGATGTTAAAAAACTGCTCGATGAGGATGAATACCATAAGCTTCAGGT
TTTTATGGCTCAGCATCCAGATTGTGGTGATGTCATTCAGGAAACGGGCGGCCTGAGAAAAATGCGCTGGGGAGCGCGAG
GCAAAGGAAAGCGTAGTGGCGTGCGAATTATCTATTTTCACCGTAGTCAACGGTATGAGATTCGCTTGCTTCTGATTTAT
CAAAAAGGCATTAAAGATGATCTCACGCCGCAGGAAAAAGCGGTGCTTCGTATGCTGAATGAGAGGTGGTAG

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9405.80 Da        Isoelectric Point: 5.0758

>AT210005 WP_000729703.1 NZ_CP079770:2500627-2500887 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGIDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT210005 NZ_CP104362:c4494919-4494629 [Salmonella enterica]
ATGGATAAAGTGTTATTTGAGCGATTAACTCAAAGTATGTCTCAAATGAATGAAATCATTGAAGGGACCCGTGAGCCTTC
TCGTACCTTTCATATTGATGCAATGAAGATTAAAGAAATACGGCAGGCATCTGGGTTGTCGCAATCTAAGTTTGCAGAAC
TGATTTCGGTCAACGTGGATACGCTGCGCAACTGGGAGCAAGGAAGACGTTCACCGACAGGGCCAGCCAAAGCGTTACTT
CGCGCCATTGCCAACGACCCGAGAAACGTTATACAAGCATTGCGTTATTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4Q2U

Antitoxin

Source ID Structure
PDB 4Q2U
AlphaFold DB A0A5F1F716

References