Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 828744..829438 Replicon chromosome
Accession NZ_CP010152
Organism Escherichia coli strain D9

Toxin (Protein)


Gene name yafO Uniprot ID A0A3P5GWS5
Locus tag RG42_RS04250 Protein ID WP_001263488.1
Coordinates 829040..829438 (+) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID S1QAE3
Locus tag RG42_RS04245 Protein ID WP_000554758.1
Coordinates 828744..829037 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
RG42_RS04220 824376..824873 + 498 WP_000006255.1 REP-associated tyrosine transposase RayT -
RG42_RS04230 825097..826809 - 1713 Protein_736 flagellar biosynthesis protein FlhA -
RG42_RS04235 826781..827566 + 786 WP_000207552.1 putative lateral flagellar export/assembly protein LafU -
RG42_RS04240 827637..828692 + 1056 WP_001226164.1 DNA polymerase IV -
RG42_RS04245 828744..829037 + 294 WP_000554758.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
RG42_RS04250 829040..829438 + 399 WP_001263488.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
RG42_RS04255 829448..829900 + 453 WP_001059892.1 GNAT family N-acetyltransferase -
RG42_RS04265 830218..830424 + 207 Protein_742 RtcB family protein -
RG42_RS04270 830420..830941 + 522 Protein_743 peptide chain release factor H -
RG42_RS04275 830998..832455 - 1458 WP_001292994.1 cytosol nonspecific dipeptidase -
RG42_RS04280 832716..833174 + 459 WP_001291990.1 xanthine phosphoribosyltransferase -
- 833770..833850 + 81 NuclAT_12 - -
- 833770..833850 + 81 NuclAT_12 - -
- 833770..833850 + 81 NuclAT_12 - -
- 833770..833850 + 81 NuclAT_12 - -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - gmhA/lpcA 814981..830941 15960


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15559.90 Da        Isoelectric Point: 6.9798

>T50884 WP_001263488.1 NZ_CP010152:829040-829438 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANENNPFPPQLRQFSRT
NDEAHLVYCQEAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

>T50884 NZ_CP010152:829040-829438 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCCTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGAGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGAGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11233.76 Da        Isoelectric Point: 5.2170

>AT50884 WP_000554758.1 NZ_CP010152:828744-829037 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Download         Length: 294 bp

>AT50884 NZ_CP010152:828744-829037 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGCGCT
GAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A3P5GWS5

Antitoxin

Source ID Structure
AlphaFold DB S1QAE3

References