Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 1144018..1144712 Replicon chromosome
Accession NZ_CP079992
Organism Escherichia coli strain KMS2001

Toxin (Protein)


Gene name yafO Uniprot ID Q47157
Locus tag KYK85_RS05480 Protein ID WP_001263489.1
Coordinates 1144314..1144712 (+) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID S1QAE3
Locus tag KYK85_RS05475 Protein ID WP_000554758.1
Coordinates 1144018..1144311 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KYK85_RS05455 (1139650) 1139650..1140147 + 498 WP_000006255.1 REP-associated tyrosine transposase RayT -
KYK85_RS05460 (1140371) 1140371..1142083 - 1713 Protein_1073 flagellar biosynthesis protein FlhA -
KYK85_RS05465 (1142055) 1142055..1142840 + 786 WP_000207552.1 putative lateral flagellar export/assembly protein LafU -
KYK85_RS05470 (1142911) 1142911..1143966 + 1056 WP_001226164.1 DNA polymerase IV -
KYK85_RS05475 (1144018) 1144018..1144311 + 294 WP_000554758.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
KYK85_RS05480 (1144314) 1144314..1144712 + 399 WP_001263489.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
KYK85_RS05485 (1144722) 1144722..1145174 + 453 WP_001059892.1 GNAT family N-acetyltransferase -
KYK85_RS05490 (1145492) 1145492..1145698 + 207 Protein_1079 RtcB family protein -
KYK85_RS05495 (1145694) 1145694..1146215 + 522 Protein_1080 peptide chain release factor H -
KYK85_RS05500 (1146272) 1146272..1147729 - 1458 WP_001292994.1 cytosol nonspecific dipeptidase -
KYK85_RS05505 (1147990) 1147990..1148448 + 459 WP_001291990.1 xanthine phosphoribosyltransferase -
- (1149044) 1149044..1149124 + 81 NuclAT_4 - -
- (1149044) 1149044..1149124 + 81 NuclAT_4 - -
- (1149044) 1149044..1149124 + 81 NuclAT_4 - -
- (1149044) 1149044..1149124 + 81 NuclAT_4 - -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - gmhA/lpcA 1126040..1146215 20175


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15487.84 Da        Isoelectric Point: 7.4215

>T210490 WP_001263489.1 NZ_CP079992:1144314-1144712 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANENNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

>T210490 NZ_CP104526:c3783640-3783242 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACAGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11233.76 Da        Isoelectric Point: 5.2170

>AT210490 WP_000554758.1 NZ_CP079992:1144018-1144311 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Download         Length: 294 bp

>AT210490 NZ_CP104526:c3783936-3783643 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATTACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGAGCT
GAAAAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A090J8B1

Antitoxin

Source ID Structure
AlphaFold DB S1QAE3

References