Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 995154..995848 Replicon chromosome
Accession NZ_CP104114
Organism Escherichia coli strain USDA-ARS-USMARC-51408

Toxin (Protein)


Gene name yafO Uniprot ID A0A3P5GWS5
Locus tag N1706_RS04630 Protein ID WP_001263488.1
Coordinates 995450..995848 (+) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID S1QAE3
Locus tag N1706_RS04625 Protein ID WP_000554758.1
Coordinates 995154..995447 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
N1706_RS04605 (990786) 990786..991283 + 498 WP_000006255.1 REP-associated tyrosine transposase RayT -
N1706_RS04610 (991507) 991507..993219 - 1713 Protein_879 flagellar biosynthesis protein FlhA -
N1706_RS04615 (993191) 993191..993976 + 786 WP_000207552.1 putative lateral flagellar export/assembly protein LafU -
N1706_RS04620 (994047) 994047..995102 + 1056 WP_001226164.1 DNA polymerase IV -
N1706_RS04625 (995154) 995154..995447 + 294 WP_000554758.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
N1706_RS04630 (995450) 995450..995848 + 399 WP_001263488.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
N1706_RS04635 (995858) 995858..996310 + 453 WP_001059892.1 GNAT family N-acetyltransferase -
N1706_RS04640 (996628) 996628..996834 + 207 Protein_885 RtcB family protein -
N1706_RS04645 (996830) 996830..997351 + 522 Protein_886 peptide chain release factor H -
N1706_RS04650 (997408) 997408..998865 - 1458 WP_001292994.1 cytosol nonspecific dipeptidase -
N1706_RS04655 (999126) 999126..999584 + 459 WP_001291990.1 xanthine phosphoribosyltransferase -
- (1000180) 1000180..1000260 + 81 NuclAT_11 - -
- (1000180) 1000180..1000260 + 81 NuclAT_11 - -
- (1000180) 1000180..1000260 + 81 NuclAT_11 - -
- (1000180) 1000180..1000260 + 81 NuclAT_11 - -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - gmhA/lpcA 981391..997351 15960


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15559.90 Da        Isoelectric Point: 6.9798

>T257220 WP_001263488.1 NZ_CP104114:995450-995848 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANENNPFPPQLRQFSRT
NDEAHLVYCQEAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11233.76 Da        Isoelectric Point: 5.2170

>AT257220 WP_000554758.1 NZ_CP104114:995154-995447 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Download         Length: 294 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A3P5GWS5

Antitoxin

Source ID Structure
AlphaFold DB S1QAE3

References