Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 4097697..4098391 Replicon chromosome
Accession NZ_CP117001
Organism Escherichia coli strain MLI108K2

Toxin (Protein)


Gene name yafO Uniprot ID Q47157
Locus tag NL410_RS21830 Protein ID WP_001263489.1
Coordinates 4097993..4098391 (+) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID S1QAE3
Locus tag NL410_RS21825 Protein ID WP_000554758.1
Coordinates 4097697..4097990 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NL410_RS21805 (4093329) 4093329..4093826 + 498 WP_000006255.1 REP-associated tyrosine transposase RayT -
NL410_RS21810 (4094050) 4094050..4095762 - 1713 Protein_3909 flagellar biosynthesis protein FlhA -
NL410_RS21815 (4095734) 4095734..4096519 + 786 WP_000207552.1 putative lateral flagellar export/assembly protein LafU -
NL410_RS21820 (4096590) 4096590..4097645 + 1056 WP_001226164.1 DNA polymerase IV -
NL410_RS21825 (4097697) 4097697..4097990 + 294 WP_000554758.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
NL410_RS21830 (4097993) 4097993..4098391 + 399 WP_001263489.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
NL410_RS21835 (4098401) 4098401..4098853 + 453 WP_001059892.1 GNAT family N-acetyltransferase -
NL410_RS21840 (4099171) 4099171..4099377 + 207 Protein_3915 RtcB family protein -
NL410_RS21845 (4099373) 4099373..4099894 + 522 Protein_3916 peptide chain release factor H -
NL410_RS21850 (4099951) 4099951..4101408 - 1458 WP_001292994.1 cytosol nonspecific dipeptidase -
NL410_RS21855 (4101669) 4101669..4102127 + 459 WP_001291990.1 xanthine phosphoribosyltransferase -
- (4102723) 4102723..4102803 + 81 NuclAT_11 - -
- (4102723) 4102723..4102803 + 81 NuclAT_11 - -
- (4102723) 4102723..4102803 + 81 NuclAT_11 - -
- (4102723) 4102723..4102803 + 81 NuclAT_11 - -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - gmhA/lpcA 4083934..4099894 15960


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15487.84 Da        Isoelectric Point: 7.4215

>T269726 WP_001263489.1 NZ_CP117001:4097993-4098391 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANENNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11233.76 Da        Isoelectric Point: 5.2170

>AT269726 WP_000554758.1 NZ_CP117001:4097697-4097990 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Download         Length: 294 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A090J8B1

Antitoxin

Source ID Structure
AlphaFold DB S1QAE3

References