Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 2494430..2495124 Replicon chromosome
Accession NZ_CP079770
Organism Escherichia coli strain HL15

Toxin (Protein)


Gene name yafO Uniprot ID Q47157
Locus tag KYX58_RS13940 Protein ID WP_001263489.1
Coordinates 2494430..2494828 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID S1QAE3
Locus tag KYX58_RS13945 Protein ID WP_000554758.1
Coordinates 2494831..2495124 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
- (2490018) 2490018..2490098 - 81 NuclAT_12 - -
- (2490018) 2490018..2490098 - 81 NuclAT_12 - -
- (2490018) 2490018..2490098 - 81 NuclAT_12 - -
- (2490018) 2490018..2490098 - 81 NuclAT_12 - -
KYX58_RS13915 (2490694) 2490694..2491152 - 459 WP_001291990.1 xanthine phosphoribosyltransferase -
KYX58_RS13920 (2491413) 2491413..2492870 + 1458 WP_001292994.1 cytosol nonspecific dipeptidase -
KYX58_RS13925 (2492927) 2492927..2493448 - 522 Protein_2450 peptide chain release factor H -
KYX58_RS13930 (2493444) 2493444..2493650 - 207 Protein_2451 RtcB family protein -
KYX58_RS13935 (2493968) 2493968..2494420 - 453 WP_001059892.1 GNAT family N-acetyltransferase -
KYX58_RS13940 (2494430) 2494430..2494828 - 399 WP_001263489.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
KYX58_RS13945 (2494831) 2494831..2495124 - 294 WP_000554758.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
KYX58_RS13950 (2495176) 2495176..2496231 - 1056 WP_001226164.1 DNA polymerase IV -
KYX58_RS13955 (2496302) 2496302..2497087 - 786 WP_000207552.1 putative lateral flagellar export/assembly protein LafU -
KYX58_RS13960 (2497059) 2497059..2498771 + 1713 Protein_2457 flagellar biosynthesis protein FlhA -
KYX58_RS13965 (2498995) 2498995..2499492 - 498 WP_000006255.1 REP-associated tyrosine transposase RayT -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - gmhA/lpcA 2494430..2513102 18672


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15487.84 Da        Isoelectric Point: 7.4215

>T210004 WP_001263489.1 NZ_CP079770:c2494828-2494430 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANENNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

>T210004 NZ_CP104362:c4185985-4185494 [Salmonella enterica]
ATGATCTCCACCCCTGAGCCGCTTCATGCCGGACATATTCTTACTCCGTTTTGCTGCGGTGTGGATTCTATAGATAACTG
GCTGAAACAGCGGGCGATGAAAAATCAGACCACTGGCGCGTCCCGTACCTTTGTGTGTTGTGGCAGCGATTCGAACGTAC
TGGCCTATTACTCGCTGGCGTCCAGCGCGGTCACGACGAATACCTCCCCCGGTCGCTTTCGTCGCAATATGCCTGACCCG
ATTCCGGTTGTGGTATTGGGGCGTCTGGCGGTGGATAAATCGCTTCATGGGCAGGGCGTCGCTCGGGCGCTGGTACGCGA
CGCAGGGCTGCGGGTTATTCAGGTGGCGGAGACTATCGGCATTCGTGGGATGCTGGTTCACGCCCTGTCGGATGAAGCGC
GGGAATTTTATCAGCGGGTGGGGTTTGTACCGTCGCCGATGGATCCGATGATGTTGATGGTGACGTTGGGGGATTTGGTG
GAGAGTGTTTAA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11233.76 Da        Isoelectric Point: 5.2170

>AT210004 WP_000554758.1 NZ_CP079770:c2495124-2494831 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Download         Length: 294 bp

>AT210004 NZ_CP104362:c4186275-4185982 [Salmonella enterica]
ATGCCAGCCGCAAACAGTATGGCGATGAAACGCGAAACCCTGAACCTGCGTATCAAACCCGCCGAGCGCGATCTTATTGA
TCGAGCGGCGAAAGCCAGAGGGAAAAACCGCACTGACTTTGTGCTGGAAGCTGCCCGTGCTGCCGCCGAGGAAGCGCTGA
TCGAACAACGCATCATTATGGCCGATCCAGAGGCTTATCAGGAGTTTCTCGTTCGTTTGGATCAAACACCTTCACCTAAT
GCCGCACTGCGTAAAACCATGCAGACCCCTGCGCCGTGGGAGCAGGAAAAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A090J8B1

Antitoxin

Source ID Structure
AlphaFold DB S1QAE3

References