Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 1647606..1648300 Replicon chromosome
Accession NZ_CP019005
Organism Escherichia coli strain Ecol_AZ155

Toxin (Protein)


Gene name yafO Uniprot ID Q47157
Locus tag BE948_RS10975 Protein ID WP_001263489.1
Coordinates 1647902..1648300 (+) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID S1QAE3
Locus tag BE948_RS10970 Protein ID WP_000554758.1
Coordinates 1647606..1647899 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BE948_RS10950 1643238..1643735 + 498 WP_000006255.1 REP-associated tyrosine transposase RayT -
BE948_RS10955 1643959..1645671 - 1713 Protein_1508 flagellar biosynthesis protein FlhA -
BE948_RS10960 1645643..1646428 + 786 WP_000207552.1 putative lateral flagellar export/assembly protein LafU -
BE948_RS10965 1646499..1647554 + 1056 WP_001226164.1 DNA polymerase IV -
BE948_RS10970 1647606..1647899 + 294 WP_000554758.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
BE948_RS10975 1647902..1648300 + 399 WP_001263489.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
BE948_RS10980 1648310..1648762 + 453 WP_001059892.1 GNAT family N-acetyltransferase -
BE948_RS10985 1649080..1649286 + 207 Protein_1514 RtcB family protein -
BE948_RS10990 1649282..1649803 + 522 Protein_1515 peptide chain release factor H -
BE948_RS10995 1649860..1651317 - 1458 WP_001292994.1 cytosol nonspecific dipeptidase -
BE948_RS11000 1651578..1652036 + 459 WP_001291990.1 xanthine phosphoribosyltransferase -
- 1652632..1652712 + 81 NuclAT_11 - -
- 1652632..1652712 + 81 NuclAT_11 - -
- 1652632..1652712 + 81 NuclAT_11 - -
- 1652632..1652712 + 81 NuclAT_11 - -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - gmhA/lpcA 1633843..1649803 15960


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15487.84 Da        Isoelectric Point: 7.4215

>T71374 WP_001263489.1 NZ_CP019005:1647902-1648300 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANENNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

>T71374 NZ_CP019005:1647902-1648300 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCCTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGAGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11233.76 Da        Isoelectric Point: 5.2170

>AT71374 WP_000554758.1 NZ_CP019005:1647606-1647899 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Download         Length: 294 bp

>AT71374 NZ_CP019005:1647606-1647899 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGCGCT
GAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A090J8B1

Antitoxin

Source ID Structure
AlphaFold DB S1QAE3

References