Detailed information of TA system    

experimentalExperimentally validated    

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/Xre-RelE
Location 3550374..3551044 Replicon chromosome
Accession NC_000962
Organism Mycobacterium tuberculosis H37Rv

Toxin (Protein)


Gene name higB Uniprot ID O53332
Locus tag Rv3182 Protein ID 15610318
Coordinates 3550374..3550718 (+) Length 115 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID O53333
Locus tag Rv3183 Protein ID 15610319
Coordinates 3550715..3551044 (+) Length 110 a.a.

Genomic Context

Rv3177Rv3178Rv3178aRv3179Rv3182Rv3183Rv3188Rv3189Rv3180cRv3181c
Location: 3545447..3546307 (861 bp)
Type: Others
Protein ID: NP_217693.1
Location: 3546438..3546797 (360 bp)
Type: Others
Protein ID: NP_217694.1
Location: 3547037..3547330 (294 bp)
Type: Others
Protein ID: YP_009030043.1
Location: 3547618..3548907 (1290 bp)
Type: Others
Protein ID: NP_217695.1
Location: 3550374..3550718 (345 bp)
Type: Toxin
Protein ID: NP_217698.1
Location: 3550715..3551044 (330 bp)
Type: Antitoxin
Protein ID: NP_217699.1
Location: 3554298..3554645 (348 bp)
Type: Others
Protein ID: NP_217704.1
Location: 3554642..3555262 (621 bp)
Type: Others
Protein ID: NP_217705.1
Location: 3549254..3549688 (435 bp)
Type: Others
Protein ID: NP_217696.1
Location: 3549691..3550143 (453 bp)
Type: Others
Protein ID: NP_217697.1
ToxinAntitoxinOther genes
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Locus tag
Coordinates
Strand
Size (bp)
Protein ID
Product
Description
Rv31773545447..3546307+861NP_217693.1peroxidase-
Rv31783546438..3546797+360NP_217694.1nitroreductase-
Rv3178a3547037..3547330+294YP_009030043.1hypothetical protein-
Rv31793547618..3548907+1290NP_217695.1hypothetical protein-
Rv3180c3549254..3549688-435NP_217696.1ribonuclease VapC45-
Rv3181c3549691..3550143-453NP_217697.1antitoxin VapB45-
Rv31823550374..3550718+345NP_217698.1hypothetical proteinToxin
Rv31833550715..3551044+330NP_217699.1transcriptional regulatorAntitoxin
Rv31883554298..3554645+348NP_217704.1hypothetical protein-
Rv31893554642..3555262+621NP_217705.1hypothetical protein-

Associated MGEs

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MGE
detail
Similar
MGEs
Relative
position
MGE Type
Cargo ARG
Virulence gene
Coordinates
Length (bp)
insideProphage--3547618..358049532877


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-103)

Antitoxin

Cro/C1-type helix-turn-helix domain

(38-91)


Sequences

Toxin        


Download         Length: 115 a.a.        Molecular weight: 12692.50 Da        Isoelectric Point: 5.6920

>T127 15610318 NC_000962:3550374-3550718 [Mycobacterium tuberculosis H37Rv]
MAVILLPQVERWFFALNRDAMASVTGAIDLLEMEGPTLGRPVVDKVNDSTFHNMKELRPAGTSIRILFAFDPARQAILLL
GGDKAGNWKRWYDNNIPIADQRSENWLASEHGGG

Download         Length: 345 bp

>T127 NC_000962:3550374-3550718 [Mycobacterium tuberculosis H37Rv]
TTGGCCGTGATCCTGCTCCCGCAGGTCGAACGGTGGTTCTTCGCGCTCAACAGGGATGCGATGGCCTCGGTCACCGGCGC
CATCGACCTGCTCGAAATGGAGGGGCCGACGTTGGGCCGCCCGGTGGTCGACAAAGTGAACGACTCAACGTTTCACAACA
TGAAGGAGCTGCGCCCCGCCGGCACCAGCATCCGGATCCTGTTCGCCTTCGACCCGGCCCGGCAGGCGATCCTGCTGCTG
GGCGGTGACAAGGCAGGCAACTGGAAACGCTGGTACGACAACAACATTCCAATCGCTGACCAGCGCTCCGAGAACTGGCT
GGCGAGCGAGCACGGAGGTGGATGA

Antitoxin


Download         Length: 110 a.a.        Molecular weight: 11802.46 Da        Isoelectric Point: 7.4051

>AT127 15610319 NC_000962:3550715-3551044 [Mycobacterium tuberculosis H37Rv]
MTMARNWRDIRADAVAQGRVDLQRAAVAREEMRDAVLAHRLAEIRKALGHARQADVAALMGVSQARVSKLESGDLSHTEL
GTLQAYVAALGGHLRIVAEFGENTVELTA

Download         Length: 330 bp

>AT127 NC_000962:3550715-3551044 [Mycobacterium tuberculosis H37Rv]
ATGACCATGGCCCGCAACTGGCGTGACATTCGCGCCGATGCCGTCGCGCAGGGCCGCGTGGATCTGCAGCGGGCCGCCGT
GGCACGCGAGGAGATGCGCGATGCCGTCCTGGCGCACCGCCTGGCCGAGATCCGCAAGGCGCTAGGCCACGCACGTCAGG
CCGACGTCGCGGCGCTGATGGGGGTCTCTCAGGCCCGTGTCTCCAAGCTGGAGAGCGGCGACCTGTCCCACACCGAACTC
GGCACCCTGCAGGCCTACGTTGCCGCCCTGGGCGGGCACCTGCGCATCGTCGCTGAGTTCGGCGAAAATACTGTCGAGCT
GACCGCCTGA

Similar Proteins

Only experimentally validated proteins are listed.

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Protein
Organism
Identities (%)
Coverage (%)
Ha-value
T10050Mycobacterium tuberculosis H37Rv

50.505

86.842

0.439

T10169Acinetobacter baumannii

44.545

96.491

0.43
Showing 1 to 2 of 2 rows

Multiple sequence alignment    


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Protein
Organism
Identities (%)
Coverage (%)
Ha-value
AT10050Mycobacterium tuberculosis H37Rv

42.991

100

0.455
Showing 1 to 1 of 1 rows

Multiple sequence alignment    




Structures

Toxin

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Source
ID
Structure
AlphaFold DBO53332

Antitoxin

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Source
ID
Structure
PDB6LTY
PDB6LTZ
AlphaFold DBO53333

References

(1) Jin-Young Park et al. (2020) Induced DNA bending by unique dimerization of HigA antitoxin. IUCrJ 7(Pt 4):748-760. [PubMed:32695421] 3D_structure


3D_structure protein structure literature