289895

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 3544052..3544722 Replicon chromosome
Accession NZ_LR882499
Organism Mycobacterium tuberculosis variant microti OV254 isolate vole

Toxin (Protein)


Gene name higB Uniprot ID O53332
Locus tag JN986_RS16525 Protein ID WP_003899954.1
Coordinates 3544052..3544396 (+) Length 115 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID G0THF6
Locus tag JN986_RS16530 Protein ID WP_003899955.1
Coordinates 3544393..3544722 (+) Length 110 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JN986_RS16500 3539357..3539650 + 294 WP_003416635.1 hypothetical protein -
JN986_RS16510 3541392..3542585 + 1194 Protein_3259 ATP-binding protein -
JN986_RS16515 3542932..3543366 - 435 WP_003899952.1 type II toxin-antitoxin system VapC family toxin -
JN986_RS16520 3543369..3543821 - 453 WP_003899953.1 type II toxin-antitoxin system Phd/YefM family antitoxin -
JN986_RS16525 3544052..3544396 + 345 WP_003899954.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
JN986_RS16530 3544393..3544722 + 330 WP_003899955.1 XRE family transcriptional regulator Antitoxin
JN986_RS16535 3545084..3546345 + 1262 WP_105799563.1 IS3-like element IS987 family transposase -
JN986_RS16540 3546550..3547815 - 1266 WP_003909837.1 hypothetical protein -
JN986_RS16545 3547983..3548192 + 210 WP_003416778.1 hypothetical protein -
JN986_RS16550 3548439..3549473 - 1035 WP_003416786.1 IS30 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - - 3539938..3571633 31695
- inside IScluster/Tn - - 3540079..3546345 6266


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-103)

Antitoxin

Cro/C1-type helix-turn-helix domain

(38-91)


Sequences

Toxin        


Download         Length: 115 a.a.        Molecular weight: 12692.50 Da        Isoelectric Point: 5.6920

>T289895 WP_003899954.1 NZ_LR882499:3544052-3544396 [Mycobacterium tuberculosis variant microti OV254]
MAVILLPQVERWFFALNRDAMASVTGAIDLLEMEGPTLGRPVVDKVNDSTFHNMKELRPAGTSIRILFAFDPARQAILLL
GGDKAGNWKRWYDNNIPIADQRSENWLASEHGGG

Download         Length: 345 bp

Antitoxin


Download         Length: 110 a.a.        Molecular weight: 11802.46 Da        Isoelectric Point: 7.4051

>AT289895 WP_003899955.1 NZ_LR882499:3544393-3544722 [Mycobacterium tuberculosis variant microti OV254]
MTMARNWRDIRADAVAQGRVDLQRAAVAREEMRDAVLAHRLAEIRKALGHARQADVAALMGVSQARVSKLESGDLSHTEL
GTLQAYVAALGGHLRIVAEFGENTVELTA

Download         Length: 330 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U4FBK2

Antitoxin

Source ID Structure
PDB 6LTY
PDB 6LTZ
AlphaFold DB G0THF6

References