Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 3550170..3550840 Replicon chromosome
Accession NZ_CP023583
Organism Mycobacterium tuberculosis strain MDRDM260

Toxin (Protein)


Gene name higB Uniprot ID O53332
Locus tag CP439_RS17410 Protein ID WP_003899954.1
Coordinates 3550170..3550514 (+) Length 115 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID G0THF6
Locus tag CP439_RS17415 Protein ID WP_003899955.1
Coordinates 3550511..3550840 (+) Length 110 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CP439_RS17375 3545243..3546103 + 861 WP_003416628.1 alpha/beta hydrolase -
CP439_RS17380 3546078..3546593 + 516 WP_003899950.1 nitroreductase family deazaflavin-dependent oxidoreductase -
CP439_RS17390 3546833..3547126 + 294 WP_003416635.1 hypothetical protein -
CP439_RS17395 3547414..3548703 + 1290 WP_003416640.1 ATP-binding protein -
CP439_RS17400 3549050..3549484 - 435 WP_003899952.1 type II toxin-antitoxin system VapC family toxin -
CP439_RS17405 3549487..3549939 - 453 WP_003899953.1 type II toxin-antitoxin system Phd/YefM family antitoxin -
CP439_RS17410 3550170..3550514 + 345 WP_003899954.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
CP439_RS17415 3550511..3550840 + 330 WP_003899955.1 XRE family transcriptional regulator Antitoxin
CP439_RS17420 3551077..3552338 + 1262 WP_096868035.1 IS3-like element IS987 family transposase -
CP439_RS17425 3552560..3553821 + 1262 WP_096868035.1 IS3-like element IS987 family transposase -
CP439_RS17430 3554094..3554441 + 348 WP_003899956.1 DUF2384 domain-containing protein -
CP439_RS17435 3554438..3555058 + 621 WP_003899957.1 RES family NAD+ phosphorylase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - - 3547414..3576832 29418


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-103)

Antitoxin

Cro/C1-type helix-turn-helix domain

(38-91)


Sequences

Toxin        


Download         Length: 115 a.a.        Molecular weight: 12692.50 Da        Isoelectric Point: 5.6920

>T82326 WP_003899954.1 NZ_CP023583:3550170-3550514 [Mycobacterium tuberculosis]
MAVILLPQVERWFFALNRDAMASVTGAIDLLEMEGPTLGRPVVDKVNDSTFHNMKELRPAGTSIRILFAFDPARQAILLL
GGDKAGNWKRWYDNNIPIADQRSENWLASEHGGG

Download         Length: 345 bp

>T82326 NZ_CP023583:3550170-3550514 [Mycobacterium tuberculosis]
TTGGCCGTGATCCTGCTCCCGCAGGTCGAACGGTGGTTCTTCGCGCTCAACAGGGATGCGATGGCCTCGGTCACCGGCGC
CATCGACCTGCTCGAAATGGAGGGGCCGACGTTGGGCCGCCCGGTGGTCGACAAAGTGAACGACTCAACGTTTCACAACA
TGAAGGAGCTGCGCCCCGCCGGCACCAGCATCCGGATCCTGTTCGCCTTCGACCCGGCCCGGCAGGCGATCCTGCTGCTG
GGCGGTGACAAGGCAGGCAACTGGAAACGCTGGTACGACAACAACATTCCAATCGCTGACCAGCGCTCCGAGAACTGGCT
GGCGAGCGAGCACGGAGGTGGATGA

Antitoxin


Download         Length: 110 a.a.        Molecular weight: 11802.46 Da        Isoelectric Point: 7.4051

>AT82326 WP_003899955.1 NZ_CP023583:3550511-3550840 [Mycobacterium tuberculosis]
MTMARNWRDIRADAVAQGRVDLQRAAVAREEMRDAVLAHRLAEIRKALGHARQADVAALMGVSQARVSKLESGDLSHTEL
GTLQAYVAALGGHLRIVAEFGENTVELTA

Download         Length: 330 bp

>AT82326 NZ_CP023583:3550511-3550840 [Mycobacterium tuberculosis]
ATGACCATGGCCCGCAACTGGCGTGACATTCGCGCCGATGCCGTCGCGCAGGGCCGCGTGGATCTGCAGCGGGCCGCCGT
GGCACGCGAGGAGATGCGCGATGCCGTCCTGGCGCACCGCCTGGCCGAGATCCGCAAGGCGCTAGGCCACGCACGTCAGG
CCGACGTCGCGGCGCTGATGGGGGTCTCTCAGGCCCGTGTCTCCAAGCTGGAGAGCGGCGACCTGTCCCACACCGAACTC
GGCACCCTGCAGGCCTACGTTGCCGCCCTGGGCGGGCACCTGCGCATCGTCGCTGAGTTCGGCGAAAATACTGTCGAGCT
GACCGCCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U4FBK2

Antitoxin

Source ID Structure
PDB 6LTY
PDB 6LTZ
AlphaFold DB G0THF6

References