Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 3569309..3569979 Replicon chromosome
Accession NZ_CP069067
Organism Mycobacterium tuberculosis strain N1268

Toxin (Protein)


Gene name higB Uniprot ID O53332
Locus tag JN146_RS16920 Protein ID WP_003899954.1
Coordinates 3569309..3569653 (+) Length 115 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID G0THF6
Locus tag JN146_RS16925 Protein ID WP_003899955.1
Coordinates 3569650..3569979 (+) Length 110 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JN146_RS16890 3564390..3564601 + 212 Protein_3333 (R)-hydratase -
JN146_RS16895 3564614..3564907 + 294 WP_003416635.1 hypothetical protein -
JN146_RS16900 3565106..3566366 + 1261 Protein_3335 IS3-like element IS987 family transposase -
JN146_RS16905 3566553..3567842 + 1290 WP_244748627.1 ATP-binding protein -
JN146_RS16910 3568189..3568623 - 435 WP_003899952.1 type II toxin-antitoxin system VapC family toxin -
JN146_RS16915 3568626..3569078 - 453 WP_003899953.1 type II toxin-antitoxin system Phd/YefM family antitoxin -
JN146_RS16920 3569309..3569653 + 345 WP_003899954.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
JN146_RS16925 3569650..3569979 + 330 WP_003899955.1 XRE family transcriptional regulator Antitoxin
JN146_RS16930 3570517..3570864 + 348 WP_003899956.1 DUF2384 domain-containing protein -
JN146_RS16935 3570861..3571481 + 621 WP_003899957.1 RES family NAD+ phosphorylase -
JN146_RS16940 3571641..3572906 - 1266 WP_003909837.1 hypothetical protein -
JN146_RS16945 3573074..3573283 + 210 WP_003416778.1 hypothetical protein -
JN146_RS16950 3573530..3574564 - 1035 WP_003416786.1 IS30 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - - 3563024..3596720 33696


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-103)

Antitoxin

Cro/C1-type helix-turn-helix domain

(38-91)


Sequences

Toxin        


Download         Length: 115 a.a.        Molecular weight: 12692.50 Da        Isoelectric Point: 5.6920

>T189211 WP_003899954.1 NZ_CP069067:3569309-3569653 [Mycobacterium tuberculosis]
MAVILLPQVERWFFALNRDAMASVTGAIDLLEMEGPTLGRPVVDKVNDSTFHNMKELRPAGTSIRILFAFDPARQAILLL
GGDKAGNWKRWYDNNIPIADQRSENWLASEHGGG

Download         Length: 345 bp

>T189211 NZ_CP090694:c1178190-1177891 [Burkholderia multivorans]
ATGCCGGTTAAGGCGCGCACGGTACGCCTGACGCCGCTGGCGGAGGCCGACCTCGAGGCCATCTGGGCTTACACGTACGA
GCGCTGGTCGCTCGACCAGGCTGAGCGCTATATCGGCGAGCTGAGCGCCGCGTTCGAGCGGCTGGCGCGCCGGGAGTCGG
TAGGGTGGCCGAGCCGCGCGGGCGGCAACTATTCGCGCTACCTGGTCGGTTCGCACGTGGCGTTCTATCGGGAAACGACC
GAAACCCTTGACGTCATTCGCGTGCTGCACCAGCGGATGGATGTCGACCGACATCTGTAA

Antitoxin


Download         Length: 110 a.a.        Molecular weight: 11802.46 Da        Isoelectric Point: 7.4051

>AT189211 WP_003899955.1 NZ_CP069067:3569650-3569979 [Mycobacterium tuberculosis]
MTMARNWRDIRADAVAQGRVDLQRAAVAREEMRDAVLAHRLAEIRKALGHARQADVAALMGVSQARVSKLESGDLSHTEL
GTLQAYVAALGGHLRIVAEFGENTVELTA

Download         Length: 330 bp

>AT189211 NZ_CP090694:c1178425-1178180 [Burkholderia multivorans]
ATGGCACGAAATACATCGATCTCGCTGGGCGATCATCTGGCCGAATTTGTCGACATGCAGGTCGCGTCCGGCCGCTACGG
CTCGGCTAGCGATGTCGTGCGCGCGGGCCTGCGCCTGCTCGAGACACAGGAAACCGAGCTGCGGGCGCTGCAGGAAGCGT
TGAAGGCGGGCGAGGCATCGGGCACACCGGCTCCGTTCGACAGCCAGGCGTTCCTGGCCCGGATGCGGGCCAAGCATGCC
GGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U4FBK2

Antitoxin

Source ID Structure
PDB 6LTY
PDB 6LTZ
AlphaFold DB G0THF6

References