Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 3550371..3551041 Replicon chromosome
Accession NC_018143
Organism Mycobacterium tuberculosis H37Rv

Toxin (Protein)


Gene name higB Uniprot ID O53332
Locus tag RVBD_RS16805 Protein ID WP_003899954.1
Coordinates 3550371..3550715 (+) Length 115 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID G0THF6
Locus tag RVBD_RS16810 Protein ID WP_003899955.1
Coordinates 3550712..3551041 (+) Length 110 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
RVBD_RS16775 3545444..3546304 + 861 WP_003416628.1 alpha/beta hydrolase -
RVBD_RS16780 3546399..3546794 + 396 WP_071854247.1 nitroreductase family deazaflavin-dependent oxidoreductase -
RVBD_RS16785 3547034..3547327 + 294 WP_003416635.1 hypothetical protein -
RVBD_RS16790 3547615..3548904 + 1290 WP_003416640.1 ATP-binding protein -
RVBD_RS16795 3549251..3549685 - 435 WP_003899952.1 type II toxin-antitoxin system VapC family toxin -
RVBD_RS16800 3549688..3550140 - 453 WP_003899953.1 type II toxin-antitoxin system Phd/YefM family antitoxin -
RVBD_RS16805 3550371..3550715 + 345 WP_003899954.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
RVBD_RS16810 3550712..3551041 + 330 WP_003899955.1 XRE family transcriptional regulator Antitoxin
RVBD_RS16820 3551278..3552539 + 1262 WP_087902221.1 IS3-like element IS987 family transposase -
RVBD_RS16830 3552761..3554022 + 1262 WP_087902221.1 IS3-like element IS987 family transposase -
RVBD_RS16835 3554295..3554642 + 348 WP_003899956.1 DUF2384 domain-containing protein -
RVBD_RS16840 3554639..3555259 + 621 WP_003899957.1 RES family NAD+ phosphorylase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - - 3547615..3580492 32877


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-103)

Antitoxin

Cro/C1-type helix-turn-helix domain

(38-91)


Sequences

Toxin        


Download         Length: 115 a.a.        Molecular weight: 12692.50 Da        Isoelectric Point: 5.6920

>T28112 WP_003899954.1 NC_018143:3550371-3550715 [Mycobacterium tuberculosis H37Rv]
MAVILLPQVERWFFALNRDAMASVTGAIDLLEMEGPTLGRPVVDKVNDSTFHNMKELRPAGTSIRILFAFDPARQAILLL
GGDKAGNWKRWYDNNIPIADQRSENWLASEHGGG

Download         Length: 345 bp

>T28112 NC_018143:3550371-3550715 [Mycobacterium tuberculosis H37Rv]
TTGGCCGTGATCCTGCTCCCGCAGGTCGAACGGTGGTTCTTCGCGCTCAACAGGGATGCGATGGCCTCGGTCACCGGCGC
CATCGACCTGCTCGAAATGGAGGGGCCGACGTTGGGCCGCCCGGTGGTCGACAAAGTGAACGACTCAACGTTTCACAACA
TGAAGGAGCTGCGCCCCGCCGGCACCAGCATCCGGATCCTGTTCGCCTTCGACCCGGCCCGGCAGGCGATCCTGCTGCTG
GGCGGTGACAAGGCAGGCAACTGGAAACGCTGGTACGACAACAACATTCCAATCGCTGACCAGCGCTCCGAGAACTGGCT
GGCGAGCGAGCACGGAGGTGGATGA

Antitoxin


Download         Length: 110 a.a.        Molecular weight: 11802.46 Da        Isoelectric Point: 7.4051

>AT28112 WP_003899955.1 NC_018143:3550712-3551041 [Mycobacterium tuberculosis H37Rv]
MTMARNWRDIRADAVAQGRVDLQRAAVAREEMRDAVLAHRLAEIRKALGHARQADVAALMGVSQARVSKLESGDLSHTEL
GTLQAYVAALGGHLRIVAEFGENTVELTA

Download         Length: 330 bp

>AT28112 NC_018143:3550712-3551041 [Mycobacterium tuberculosis H37Rv]
ATGACCATGGCCCGCAACTGGCGTGACATTCGCGCCGATGCCGTCGCGCAGGGCCGCGTGGATCTGCAGCGGGCCGCCGT
GGCACGCGAGGAGATGCGCGATGCCGTCCTGGCGCACCGCCTGGCCGAGATCCGCAAGGCGCTAGGCCACGCACGTCAGG
CCGACGTCGCGGCGCTGATGGGGGTCTCTCAGGCCCGTGTCTCCAAGCTGGAGAGCGGCGACCTGTCCCACACCGAACTC
GGCACCCTGCAGGCCTACGTTGCCGCCCTGGGCGGGCACCTGCGCATCGTCGCTGAGTTCGGCGAAAATACTGTCGAGCT
GACCGCCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U4FBK2

Antitoxin

Source ID Structure
PDB 6LTY
PDB 6LTZ
AlphaFold DB G0THF6

References