65389

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 3549378..3550048 Replicon chromosome
Accession NZ_CP016888
Organism Mycobacterium tuberculosis strain SCAID 252.0

Toxin (Protein)


Gene name higB Uniprot ID O53332
Locus tag BEE64_RS16800 Protein ID WP_003899954.1
Coordinates 3549378..3549722 (+) Length 115 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID G0THF6
Locus tag BEE64_RS16805 Protein ID WP_003899955.1
Coordinates 3549719..3550048 (+) Length 110 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BEE64_RS16770 3544683..3544976 + 294 WP_003416635.1 hypothetical protein -
BEE64_RS16775 3545264..3546553 + 1290 WP_003416640.1 ATP-binding protein -
BEE64_RS16790 3548258..3548692 - 435 WP_003899952.1 type II toxin-antitoxin system VapC family toxin -
BEE64_RS16795 3548695..3549147 - 453 WP_003899953.1 type II toxin-antitoxin system Phd/YefM family antitoxin -
BEE64_RS16800 3549378..3549722 + 345 WP_003899954.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
BEE64_RS16805 3549719..3550048 + 330 WP_003899955.1 XRE family transcriptional regulator Antitoxin
BEE64_RS16815 3550282..3551543 + 1262 WP_087902221.1 IS3-like element IS987 family transposase -
BEE64_RS16825 3551765..3553026 + 1262 WP_087902221.1 IS3-like element IS987 family transposase -
BEE64_RS16830 3553303..3553650 + 348 WP_003899956.1 DUF2384 domain-containing protein -
BEE64_RS16835 3553647..3554267 + 621 WP_003899957.1 RES family NAD+ phosphorylase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - - 3545264..3576041 30777
- inside IScluster/Tn - - 3546888..3553026 6138


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-103)

Antitoxin

Cro/C1-type helix-turn-helix domain

(38-91)


Sequences

Toxin        


Download         Length: 115 a.a.        Molecular weight: 12692.50 Da        Isoelectric Point: 5.6920

>T65389 WP_003899954.1 NZ_CP016888:3549378-3549722 [Mycobacterium tuberculosis]
MAVILLPQVERWFFALNRDAMASVTGAIDLLEMEGPTLGRPVVDKVNDSTFHNMKELRPAGTSIRILFAFDPARQAILLL
GGDKAGNWKRWYDNNIPIADQRSENWLASEHGGG

Download         Length: 345 bp

>T65389 NZ_CP016888:3549378-3549722 [Mycobacterium tuberculosis]
TTGGCCGTGATCCTGCTCCCGCAGGTCGAACGGTGGTTCTTCGCGCTCAACAGGGATGCGATGGCCTCGGTCACCGGCGC
CATCGACCTGCTCGAAATGGAGGGGCCGACGTTGGGCCGCCCGGTGGTCGACAAAGTGAACGACTCAACGTTTCACAACA
TGAAGGAGCTGCGCCCCGCCGGCACCAGCATCCGGATCCTGTTCGCCTTCGACCCGGCCCGGCAGGCGATCCTGCTGCTG
GGCGGTGACAAGGCAGGCAACTGGAAACGCTGGTACGACAACAACATTCCAATCGCTGACCAGCGCTCCGAGAACTGGCT
GGCGAGCGAGCACGGAGGTGGATGA

Antitoxin


Download         Length: 110 a.a.        Molecular weight: 11802.46 Da        Isoelectric Point: 7.4051

>AT65389 WP_003899955.1 NZ_CP016888:3549719-3550048 [Mycobacterium tuberculosis]
MTMARNWRDIRADAVAQGRVDLQRAAVAREEMRDAVLAHRLAEIRKALGHARQADVAALMGVSQARVSKLESGDLSHTEL
GTLQAYVAALGGHLRIVAEFGENTVELTA

Download         Length: 330 bp

>AT65389 NZ_CP016888:3549719-3550048 [Mycobacterium tuberculosis]
ATGACCATGGCCCGCAACTGGCGTGACATTCGCGCCGATGCCGTCGCGCAGGGCCGCGTGGATCTGCAGCGGGCGGCCGT
GGCACGCGAGGAGATGCGCGATGCCGTCCTGGCGCACCGCCTGGCCGAGATCCGCAAGGCGCTAGGCCACGCACGTCAGG
CCGACGTCGCGGCGCTGATGGGGGTCTCTCAGGCCCGTGTCTCCAAGCTGGAGAGCGGCGACCTGTCCCACACCGAACTC
GGCACCCTGCAGGCCTACGTTGCCGCCCTGGGCGGGCACCTGCGCATCGTCGCTGAGTTCGGCGAAAATACTGTCGAGCT
GACCGCCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U4FBK2

Antitoxin

Source ID Structure
AlphaFold DB G0THF6

References