Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 3550156..3550826 Replicon chromosome
Accession NZ_CP023622
Organism Mycobacterium tuberculosis strain MDRDM827

Toxin (Protein)


Gene name higB Uniprot ID O53332
Locus tag CP478_RS17420 Protein ID WP_003899954.1
Coordinates 3550156..3550500 (+) Length 115 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID G0THF6
Locus tag CP478_RS17425 Protein ID WP_003899955.1
Coordinates 3550497..3550826 (+) Length 110 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CP478_RS17385 3545229..3546089 + 861 WP_003416628.1 alpha/beta hydrolase -
CP478_RS17390 3546064..3546579 + 516 WP_003899950.1 nitroreductase family deazaflavin-dependent oxidoreductase -
CP478_RS17400 3546819..3547112 + 294 WP_003416635.1 hypothetical protein -
CP478_RS17405 3547400..3548689 + 1290 WP_003906996.1 ATP-binding protein -
CP478_RS17410 3549036..3549470 - 435 WP_003899952.1 type II toxin-antitoxin system VapC family toxin -
CP478_RS17415 3549473..3549925 - 453 WP_003899953.1 type II toxin-antitoxin system Phd/YefM family antitoxin -
CP478_RS17420 3550156..3550500 + 345 WP_003899954.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
CP478_RS17425 3550497..3550826 + 330 WP_003899955.1 XRE family transcriptional regulator Antitoxin
CP478_RS17430 3551063..3552324 + 1262 WP_087902221.1 IS3-like element IS987 family transposase -
CP478_RS17435 3552546..3553807 + 1262 WP_087902221.1 IS3-like element IS987 family transposase -
CP478_RS17440 3554080..3554427 + 348 WP_003899956.1 DUF2384 domain-containing protein -
CP478_RS17445 3554424..3555044 + 621 WP_003899957.1 RES family NAD+ phosphorylase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - - 3547400..3576819 29419


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-103)

Antitoxin

Cro/C1-type helix-turn-helix domain

(38-91)


Sequences

Toxin        


Download         Length: 115 a.a.        Molecular weight: 12692.50 Da        Isoelectric Point: 5.6920

>T84699 WP_003899954.1 NZ_CP023622:3550156-3550500 [Mycobacterium tuberculosis]
MAVILLPQVERWFFALNRDAMASVTGAIDLLEMEGPTLGRPVVDKVNDSTFHNMKELRPAGTSIRILFAFDPARQAILLL
GGDKAGNWKRWYDNNIPIADQRSENWLASEHGGG

Download         Length: 345 bp

>T84699 NZ_CP023622:3550156-3550500 [Mycobacterium tuberculosis]
TTGGCCGTGATCCTGCTCCCGCAGGTCGAACGGTGGTTCTTCGCGCTCAACAGGGATGCGATGGCCTCGGTCACCGGCGC
CATCGACCTGCTCGAAATGGAGGGGCCGACGTTGGGCCGCCCGGTGGTCGACAAAGTGAACGACTCAACGTTTCACAACA
TGAAGGAGCTGCGCCCCGCCGGCACCAGCATCCGGATCCTGTTCGCCTTCGACCCGGCCCGGCAGGCGATCCTGCTGCTG
GGCGGTGACAAGGCAGGCAACTGGAAACGCTGGTACGACAACAACATTCCAATCGCTGACCAGCGCTCCGAGAACTGGCT
GGCGAGCGAGCACGGAGGTGGATGA

Antitoxin


Download         Length: 110 a.a.        Molecular weight: 11802.46 Da        Isoelectric Point: 7.4051

>AT84699 WP_003899955.1 NZ_CP023622:3550497-3550826 [Mycobacterium tuberculosis]
MTMARNWRDIRADAVAQGRVDLQRAAVAREEMRDAVLAHRLAEIRKALGHARQADVAALMGVSQARVSKLESGDLSHTEL
GTLQAYVAALGGHLRIVAEFGENTVELTA

Download         Length: 330 bp

>AT84699 NZ_CP023622:3550497-3550826 [Mycobacterium tuberculosis]
ATGACCATGGCCCGCAACTGGCGTGACATTCGCGCCGATGCCGTCGCGCAGGGCCGCGTGGATCTGCAGCGGGCCGCCGT
GGCACGCGAGGAGATGCGCGATGCCGTCCTGGCGCACCGCCTGGCCGAGATCCGCAAGGCGCTAGGCCACGCACGTCAGG
CCGACGTCGCGGCGCTGATGGGGGTCTCTCAGGCCCGTGTCTCCAAGCTGGAGAGCGGCGACCTGTCCCACACCGAACTC
GGCACCCTGCAGGCCTACGTTGCCGCCCTGGGCGGGCACCTGCGCATCGTCGCTGAGTTCGGCGAAAATACTGTCGAGCT
GACCGCCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U4FBK2

Antitoxin

Source ID Structure
PDB 6LTY
PDB 6LTZ
AlphaFold DB G0THF6

References