Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 3549756..3550426 Replicon chromosome
Accession NZ_CP007027
Organism Mycobacterium tuberculosis H37RvSiena

Toxin (Protein)


Gene name higB Uniprot ID O53332
Locus tag Y980_RS16815 Protein ID WP_003899954.1
Coordinates 3549756..3550100 (+) Length 115 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID G0THF6
Locus tag Y980_RS16820 Protein ID WP_003899955.1
Coordinates 3550097..3550426 (+) Length 110 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
Y980_RS16785 3544829..3545689 + 861 WP_003416628.1 alpha/beta hydrolase -
Y980_RS16790 3545664..3546179 + 516 WP_003899950.1 nitroreductase family deazaflavin-dependent oxidoreductase -
Y980_RS16795 3546419..3546712 + 294 WP_003416635.1 hypothetical protein -
Y980_RS16800 3547000..3548289 + 1290 WP_003416640.1 ATP-binding protein -
Y980_RS16805 3548636..3549070 - 435 WP_003899952.1 type II toxin-antitoxin system VapC family toxin -
Y980_RS16810 3549073..3549525 - 453 WP_003899953.1 type II toxin-antitoxin system Phd/YefM family antitoxin -
Y980_RS16815 3549756..3550100 + 345 WP_003899954.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
Y980_RS16820 3550097..3550426 + 330 WP_003899955.1 XRE family transcriptional regulator Antitoxin
Y980_RS16830 3550663..3551924 + 1262 WP_087902221.1 IS3-like element IS987 family transposase -
Y980_RS16840 3552146..3553407 + 1262 WP_087902221.1 IS3-like element IS987 family transposase -
Y980_RS16845 3553680..3554027 + 348 WP_003899956.1 DUF2384 domain-containing protein -
Y980_RS16850 3554024..3554644 + 621 WP_003899957.1 RES family NAD+ phosphorylase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - - 3549073..3576418 27345


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-103)

Antitoxin

Cro/C1-type helix-turn-helix domain

(38-91)


Sequences

Toxin        


Download         Length: 115 a.a.        Molecular weight: 12692.50 Da        Isoelectric Point: 5.6920

>T45934 WP_003899954.1 NZ_CP007027:3549756-3550100 [Mycobacterium tuberculosis H37RvSiena]
MAVILLPQVERWFFALNRDAMASVTGAIDLLEMEGPTLGRPVVDKVNDSTFHNMKELRPAGTSIRILFAFDPARQAILLL
GGDKAGNWKRWYDNNIPIADQRSENWLASEHGGG

Download         Length: 345 bp

>T45934 NZ_CP007027:3549756-3550100 [Mycobacterium tuberculosis H37RvSiena]
TTGGCCGTGATCCTGCTCCCGCAGGTCGAACGGTGGTTCTTCGCGCTCAACAGGGATGCGATGGCCTCGGTCACCGGCGC
CATCGACCTGCTCGAAATGGAGGGGCCGACGTTGGGCCGCCCGGTGGTCGACAAAGTGAACGACTCAACGTTTCACAACA
TGAAGGAGCTGCGCCCCGCCGGCACCAGCATCCGGATCCTGTTCGCCTTCGACCCGGCCCGGCAGGCGATCCTGCTGCTG
GGCGGTGACAAGGCAGGCAACTGGAAACGCTGGTACGACAACAACATTCCAATCGCTGACCAGCGCTCCGAGAACTGGCT
GGCGAGCGAGCACGGAGGTGGATGA

Antitoxin


Download         Length: 110 a.a.        Molecular weight: 11802.46 Da        Isoelectric Point: 7.4051

>AT45934 WP_003899955.1 NZ_CP007027:3550097-3550426 [Mycobacterium tuberculosis H37RvSiena]
MTMARNWRDIRADAVAQGRVDLQRAAVAREEMRDAVLAHRLAEIRKALGHARQADVAALMGVSQARVSKLESGDLSHTEL
GTLQAYVAALGGHLRIVAEFGENTVELTA

Download         Length: 330 bp

>AT45934 NZ_CP007027:3550097-3550426 [Mycobacterium tuberculosis H37RvSiena]
ATGACCATGGCCCGCAACTGGCGTGACATTCGCGCCGATGCCGTCGCGCAGGGCCGCGTGGATCTGCAGCGGGCCGCCGT
GGCACGCGAGGAGATGCGCGATGCCGTCCTGGCGCACCGCCTGGCCGAGATCCGCAAGGCGCTAGGCCACGCACGTCAGG
CCGACGTCGCGGCGCTGATGGGGGTCTCTCAGGCCCGTGTCTCCAAGCTGGAGAGCGGCGACCTGTCCCACACCGAACTC
GGCACCCTGCAGGCCTACGTTGCCGCCCTGGGCGGGCACCTGCGCATCGTCGCTGAGTTCGGCGAAAATACTGTCGAGCT
GACCGCCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U4FBK2

Antitoxin

Source ID Structure
PDB 6LTY
PDB 6LTZ
AlphaFold DB G0THF6

References