144816

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 3547705..3548375 Replicon chromosome
Accession NZ_CP046728
Organism Mycobacterium tuberculosis strain TCDC11

Toxin (Protein)


Gene name higB Uniprot ID O53332
Locus tag GP477_RS16690 Protein ID WP_003899954.1
Coordinates 3547705..3548049 (+) Length 115 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID G0THF6
Locus tag GP477_RS16695 Protein ID WP_003899955.1
Coordinates 3548046..3548375 (+) Length 110 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GP477_RS16670 3543591..3544880 + 1290 WP_003416640.1 ATP-binding protein -
GP477_RS16680 3546585..3547019 - 435 WP_003899952.1 type II toxin-antitoxin system VapC family toxin -
GP477_RS16685 3547022..3547474 - 453 WP_003899953.1 type II toxin-antitoxin system Phd/YefM family antitoxin -
GP477_RS16690 3547705..3548049 + 345 WP_003899954.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
GP477_RS16695 3548046..3548375 + 330 WP_003899955.1 XRE family transcriptional regulator Antitoxin
GP477_RS16700 3548913..3549260 + 348 WP_003899956.1 DUF2384 domain-containing protein -
GP477_RS16705 3549257..3549877 + 621 WP_003899957.1 RES family NAD+ phosphorylase -
GP477_RS16710 3550037..3550888 - 852 Protein_3299 hypothetical protein -
GP477_RS16715 3550945..3552206 + 1262 WP_087902221.1 IS3-like element IS987 family transposase -
GP477_RS16720 3552251..3552661 - 411 Protein_3301 hypothetical protein -
GP477_RS16725 3552829..3553038 + 210 WP_003416778.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - - 3542011..3576397 34386
- inside IScluster/Tn - - 3542011..3552206 10195


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-103)

Antitoxin

Cro/C1-type helix-turn-helix domain

(38-91)


Sequences

Toxin        


Download         Length: 115 a.a.        Molecular weight: 12692.50 Da        Isoelectric Point: 5.6920

>T144816 WP_003899954.1 NZ_CP046728:3547705-3548049 [Mycobacterium tuberculosis]
MAVILLPQVERWFFALNRDAMASVTGAIDLLEMEGPTLGRPVVDKVNDSTFHNMKELRPAGTSIRILFAFDPARQAILLL
GGDKAGNWKRWYDNNIPIADQRSENWLASEHGGG

Download         Length: 345 bp

>T144816 NZ_CP062242:c1917464-1917361 [Escherichia coli]
GGCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTGTTCGGTCTCTTTTT

Antitoxin


Download         Length: 110 a.a.        Molecular weight: 11802.46 Da        Isoelectric Point: 7.4051

>AT144816 WP_003899955.1 NZ_CP046728:3548046-3548375 [Mycobacterium tuberculosis]
MTMARNWRDIRADAVAQGRVDLQRAAVAREEMRDAVLAHRLAEIRKALGHARQADVAALMGVSQARVSKLESGDLSHTEL
GTLQAYVAALGGHLRIVAEFGENTVELTA

Download         Length: 330 bp

>AT144816 NZ_CP062242:1917323-1917594 [Escherichia coli]
AAAGTCAGCGAAGGAAATGCTTCTGGCTTTTAACAGATAAAAAGAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAA
TGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGCATTAATGCAGGCTTAGTTGCCTTGCCCTTTAAGAATAGATGACG
ACGCCAGGTTTTCCAGTTTGCGTGCAAAATGGTCAATAAAAAGCGTGGTGGTCATCAGCTGAAATGTTAAAAACCGCCCG
TTCTGGTGAAAGAACTGAGGCGGTTTTTTTAT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U4FBK2

Antitoxin

Source ID Structure
PDB 6LTY
PDB 6LTZ
AlphaFold DB G0THF6

References