229199

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 3549696..3550366 Replicon chromosome
Accession NZ_CP089780
Organism Mycobacterium tuberculosis strain M0003941-3

Toxin (Protein)


Gene name higB Uniprot ID O53332
Locus tag LVJ75_RS16805 Protein ID WP_003899954.1
Coordinates 3549696..3550040 (+) Length 115 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID G0THF6
Locus tag LVJ75_RS16810 Protein ID WP_003899955.1
Coordinates 3550037..3550366 (+) Length 110 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LVJ75_RS16785 (LVJ75_16770) 3545582..3546871 + 1290 WP_003416640.1 ATP-binding protein -
LVJ75_RS16795 (LVJ75_16780) 3548576..3549010 - 435 WP_003899952.1 type II toxin-antitoxin system VapC family toxin -
LVJ75_RS16800 (LVJ75_16785) 3549013..3549465 - 453 WP_003899953.1 type II toxin-antitoxin system Phd/YefM family antitoxin -
LVJ75_RS16805 (LVJ75_16790) 3549696..3550040 + 345 WP_003899954.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
LVJ75_RS16810 (LVJ75_16795) 3550037..3550366 + 330 WP_003899955.1 XRE family transcriptional regulator Antitoxin
LVJ75_RS16815 (LVJ75_16800) 3550904..3551251 + 348 WP_003899956.1 DUF2384 domain-containing protein -
LVJ75_RS16820 (LVJ75_16805) 3551248..3551868 + 621 WP_003899957.1 RES family NAD+ phosphorylase -
LVJ75_RS16825 (LVJ75_16810) 3552028..3553293 - 1266 WP_003902423.1 hypothetical protein -
LVJ75_RS16830 (LVJ75_16815) 3553461..3553670 + 210 WP_003416778.1 hypothetical protein -
LVJ75_RS16835 (LVJ75_16820) 3553917..3554951 - 1035 WP_003416786.1 IS30 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - - 3544003..3569149 25146


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-103)

Antitoxin

Cro/C1-type helix-turn-helix domain

(38-91)


Sequences

Toxin        


Download         Length: 115 a.a.        Molecular weight: 12692.50 Da        Isoelectric Point: 5.6920

>T229199 WP_003899954.1 NZ_CP089780:3549696-3550040 [Mycobacterium tuberculosis]
MAVILLPQVERWFFALNRDAMASVTGAIDLLEMEGPTLGRPVVDKVNDSTFHNMKELRPAGTSIRILFAFDPARQAILLL
GGDKAGNWKRWYDNNIPIADQRSENWLASEHGGG

Download         Length: 345 bp

>T229199 NZ_CP124415:c2039817-2039503 [Escherichia coli]
ATGCACCTGATAACTCAAAAAGCATTGAAAGATGCTGCGGAAAAATACCCGCAACATAAAACGGAGTTGGTGGCTCTGGG
GAACACGATTGCTAAGGGATATTTCAAAAAACCTGAGTCATTAAAAGCAGTATTCCCTTCTCTGGATAACTTCAAATATC
TGGATAAGCATTATGTTTTCAATGTCGGGGGCAATGAATTACGTGTTGTAGCAATGGTCTTTTTTGAATCGCAAAAGTGC
TACATACGTGAAGTTATGACGCATAAAGAATACGATTTCTTTACCGCCGTTCATCGTACTAAGGGGAAAAAATGA

Antitoxin


Download         Length: 110 a.a.        Molecular weight: 11802.46 Da        Isoelectric Point: 7.4051

>AT229199 WP_003899955.1 NZ_CP089780:3550037-3550366 [Mycobacterium tuberculosis]
MTMARNWRDIRADAVAQGRVDLQRAAVAREEMRDAVLAHRLAEIRKALGHARQADVAALMGVSQARVSKLESGDLSHTEL
GTLQAYVAALGGHLRIVAEFGENTVELTA

Download         Length: 330 bp

>AT229199 NZ_CP124415:c2039506-2039090 [Escherichia coli]
ATGATTGCGATTGCCGACATCTTGCAAGCAGGAGAAAAGCTAACTGCTGTGGCACCTTTTCTGGCGGGTATTCAGAACGA
GGAACAATACACCCAGGCGCTGGAACTGGTAGATCATCTGCTGCTCAACGATCCTGAAAACCCCTTACTGGATCTGGTGT
GTGCCAAAATAACCGCGTGGGAAGAATCAGCGCCCGAATTTGTGGAATTTAATGCCATGGCTCAAGCCATGCCTGGCGGT
ATAGCCGTGATTCGTACCCTTATGGATCAATATGGTTTAACCCTTTCCGATCTTCCGGAAATAGGCAGTAAATCGATGGT
GTCACGCGTTTTGAGCGGGAAAAGGAAATTAACGCTGGAACACGCTAAAAAACTGGCAACGCGATTCGGTATTTCTCCCG
CCTTGTTTATTGATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U4FBK2

Antitoxin

Source ID Structure
PDB 6LTY
PDB 6LTZ
AlphaFold DB G0THF6

References