Detailed information of TA system TA010169

Experimentally validated

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/-
• Location: 3621..4272
• Replicon: plasmid pAB120
• Accession: NC_019359
• Organism: Acinetobacter baumannii

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: HPE87_RS00025
• Protein ID: WP_000269902.1
• Coordinates: 3621..3977 (+)
• Length: 119 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: HPE87_RS00030
• Protein ID: WP_001140622.1
• Coordinates: 3970..4272 (+)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HPE87_RS00005 (pAB120_01)	322..1272	+	951	WP_001205343.1	replication initiation protein RepM	-
HPE87_RS00010 (pAB120_02)	1265..1840	+	576	WP_001096616.1	plasmid replication DNA-binding protein	-
HPE87_RS00015	1860..2003	+	144	WP_001125246.1	hypothetical protein	-
HPE87_RS00020 (pAB120_03)	2185..3012	-	828	WP_000713530.1	OXA-24 family carbapenem-hydrolyzing class D beta-lactamase OXA-72	-
HPE87_RS00025 (pAB120_04)	3621..3977	+	357	WP_000269902.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HPE87_RS00030 (pAB120_05)	3970..4272	+	303	WP_001140622.1	XRE family transcriptional regulator	Antitoxin
HPE87_RS00035 (pAB120_06)	4265..4474	+	210	WP_000069473.1	hypothetical protein	-
HPE87_RS00040 (pAB120_07)	4800..5261	-	462	WP_000761346.1	DIP1984 family protein	-
HPE87_RS00045 (pAB120_08)	5567..7978	-	2412	WP_000932953.1	TonB-dependent receptor ZnuD2	-
HPE87_RS00050 (pAB120_09)	8106..8420	-	315	WP_001983304.1	BrnA antitoxin family protein	-
HPE87_RS00055 (pAB120_10)	8407..8694	-	288	WP_000438826.1	BrnT family toxin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	Similar MGE(s) Similar MGE(s) is defined as other TA-related MGE(s) with Mash distance < 0.01 to this MGE. Detail Genome accession Organism MGE type Related TA Coordinates Mash distance View NZ_CP031446 Acinetobacter baumannii strain MDR-UNC Non-Mobilizable plasmid TA109195, TA109196 1..10879 0 View NZ_CP064193 Acinetobacter baumannii strain X4-65 Non-Mobilizable plasmid 1..8970 0.008 View NZ_CP074696 Acinetobacter baumannii strain X4-107 Non-Mobilizable plasmid TA202661 1..8961 0.008 View NZ_CP076737 Acinetobacter baumannii strain X4-201 Non-Mobilizable plasmid TA206471 1..8970 0.009 View NZ_CP076740 Acinetobacter baumannii strain X4-584 Non-Mobilizable plasmid TA206474 1..8954 0.008 View NZ_CP076743 Acinetobacter baumannii strain X4-705 Non-Mobilizable plasmid 1..8964 0.008 View NZ_CP087322 Acinetobacter baumannii strain OC043 Non-Mobilizable plasmid TA225352, TA225353, TA225354 1..21542 0.001 View NZ_CP087323 Acinetobacter baumannii strain OC043 Non-Mobilizable plasmid TA225355, TA225356 1..15932 0 View NZ_CP087324 Acinetobacter baumannii strain OC043 Non-Mobilizable plasmid 1..14741 0.003	inside	Non-Mobilizable plasmid	blaOXA-72	-	1..10879	10879

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-106)

Antitoxin

• HigA2-like, helix-turn-helix domain (13-89)

Sequences

Toxin

Protein

• Length: 119 a.a.
• Molecular weight: 13487.44 Da
• Isoelectric Point: 6.4631

>T10169 WP_000269902.1 NC_019359:3621-3977 [Acinetobacter baumannii]
MWTVITTDLFNEWLAQQDESTQEKVLAALVVLQQQGPSLGRPLVDTVYDSKFTNMKELRVQHRGKPLRAFFAFDPLRQAI
VLCIGDKGGKKRFYKEMLDIADQQYELHLSTLGDQSNG

Nucleotide

• Length: 357 bp

>T10169 NC_019359:3621-3977 [Acinetobacter baumannii]
ATGTGGACAGTCATTACAACGGATCTGTTTAACGAATGGCTTGCACAGCAAGATGAATCTACACAAGAGAAAGTACTCGC
AGCGTTGGTCGTTCTACAGCAACAGGGACCAAGTTTAGGTCGTCCTTTAGTAGATACCGTCTATGACTCCAAATTTACCA
ATATGAAAGAGCTACGTGTTCAGCATCGAGGTAAACCACTGAGAGCCTTTTTCGCATTTGATCCTTTACGACAAGCTATT
GTGCTGTGTATCGGTGATAAAGGCGGTAAAAAGCGTTTTTATAAAGAGATGTTAGATATTGCTGATCAGCAATACGAACT
TCATCTCTCAACTTTAGGAGATCAATCTAATGGCTAA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11178.98 Da
• Isoelectric Point: 6.7282

>AT10169 WP_001140622.1 NC_019359:3970-4272 [Acinetobacter baumannii]
MAKTLQELLASRSPESQARIQQMADELLLETQLHLIREELEISQKELAATLGIKQPSLSAIENRGHDLKISTMKKYVEAM
GGKLRIDVELPTGKHIGFNV

Nucleotide

• Length: 303 bp

>AT10169 NC_019359:3970-4272 [Acinetobacter baumannii]
ATGGCTAAGACCCTACAAGAATTATTGGCTAGTCGCTCTCCAGAGAGTCAGGCTCGTATTCAACAAATGGCTGATGAATT
ACTGTTGGAAACTCAGCTTCACCTTATTCGTGAAGAGTTAGAGATTTCTCAAAAAGAACTGGCTGCAACCCTTGGAATCA
AACAGCCGTCATTGTCAGCAATCGAAAATCGTGGTCATGATCTTAAAATTTCAACCATGAAAAAGTATGTTGAAGCAATG
GGCGGAAAACTTCGTATTGATGTAGAGCTTCCAACAGGAAAACACATAGGATTTAATGTCTAA

Similar Proteins

Only experimentally validated proteins are listed.

Toxin

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
T10063	Escherichia coli strain Ec158	51	100	0.51
T10050	Mycobacterium tuberculosis H37Rv	44.348	97.458	0.432
T127	Mycobacterium tuberculosis H37Rv	44.545	96.491	0.43
T6105	Burkholderia pseudomallei K96243	27.778	100	0.306
T6106	Burkholderia pseudomallei K96243	27.778	100	0.306

Multiple sequence alignment

Antitoxin

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
AT10063	Escherichia coli strain Ec158	51	100	0.51
AT10050	Mycobacterium tuberculosis H37Rv	34.375	96	0.33

Multiple sequence alignment

References

(1) Julija Armalytė et al. (2018) The higBA Toxin-Antitoxin Module From the Opportunistic Pathogen Acinetobacter baumannii - Regulation, Activity, and Evolution. Frontiers in Microbiology 9:732. [PubMed:29706946]


 experimental literature