Detailed information of TA system TA006106

Experimentally validated

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE(toxin)
• Location: 1435651..1437660
• Replicon: chromosome
• Accession: NC_006351
• Organism: Burkholderia pseudomallei K96243

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: BPS_RS24505
• Protein ID: WP_024431052.1
• Coordinates: 1437364..1437660 (+)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: BPS_RS24510
• Protein ID: WP_009971793.1
• Coordinates: 1437663..1438019 (+)
• Length: 119 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BPS_RS24460	1430773..1430895	+	123	WP_006027270.1	hypothetical protein	-
BPS_RS24465 (BPSS1052)	1430895..1432688	+	1794	WP_009971786.1	replication endonuclease	-
BPS_RS24470 (BPSS1053)	1432704..1432970	+	267	WP_009971787.1	ogr/Delta-like zinc finger family protein	-
BPS_RS24475 (BPSS1054)	1432967..1433173	+	207	WP_004532731.1	ogr/Delta-like zinc finger family protein	-
BPS_RS24480 (BPSS1055)	1433263..1433916	+	654	WP_004532770.1	AAA family ATPase	-
BPS_RS24485 (BPSS1056)	1434150..1434272	+	123	WP_009897078.1	CopG family transcriptional regulator	-
BPS_RS24490 (BPSS1057)	1434375..1434908	+	534	WP_004532793.1	bacteriophage gp29 protein	-
BPS_RS24495 (BPSS1058)	1434905..1435642	+	738	WP_011205520.1	VRR-NUC domain-containing protein	-
BPS_RS24500 (BPSS1059)	1435657..1436772	+	1116	WP_029670743.1	DUF3396 domain-containing protein	-
BPS_RS24505 (BPSS1060)	1437364..1437660	+	297	WP_024431052.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
BPS_RS24510 (BPSS1061)	1437663..1438019	+	357	WP_009971793.1	putative addiction module antidote protein	Antitoxin
BPS_RS24515 (BPSS1062)	1438063..1439118	-	1056	WP_011205524.1	phage portal protein	-
BPS_RS24520 (BPSS1063)	1439115..1440884	-	1770	WP_009972550.1	terminase ATPase subunit family protein	-
BPS_RS24525 (BPSS1064)	1441028..1441837	+	810	WP_011205526.1	GPO family capsid scaffolding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	Similar MGE(s) Similar MGE(s) is defined as other TA-related MGE(s) with Mash distance < 0.01 to this MGE. Detail Genome accession Organism MGE type Related TA Coordinates Mash distance View NZ_AP028074 Burkholderia pseudomallei strain GTC3P0050 Prophage TA044474 1418660..1456475 0.008 View NZ_AP028076 Burkholderia pseudomallei strain GTC3P0054 Prophage TA044477 1418572..1456631 0.007 View NZ_CP008912 Burkholderia pseudomallei HBPUB10134a Prophage TA047912 1066770..1105633 0.007 View NZ_CP009537 Burkholderia pseudomallei K96243 Prophage TA049221 48057..86010 0 View NZ_CP009586 Burkholderia pseudomallei strain PHLS 112 Prophage TA049297 326563..364516 0.003	inside	Prophage	-	-	1424167..1462120	37953

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (3-95)

Antitoxin

• Uncharacterized protein HI1420 (16-94)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 10916.71 Da
• Isoelectric Point: 9.9369

>T6106 WP_024431052.1 NC_006351:1437364-1437660 [Burkholderia pseudomallei K96243]
MFKVLTTPQFDKWLDGLRDPVGSAAINLRIERAKLGNLGQWRAVGDGVNEMKIDVGPGYRAYFVRREKIIVVVLCGGDKS
TQKKDIKLAKQIAGELED

Nucleotide

• Length: 297 bp

>T6106 NC_006351:1437364-1437660 [Burkholderia pseudomallei K96243]
ATGTTCAAAGTTCTGACGACCCCCCAGTTTGACAAATGGCTTGACGGGCTTCGCGATCCGGTCGGTAGCGCGGCAATCAA
CCTGCGCATCGAGCGGGCGAAGCTTGGCAATCTCGGCCAGTGGCGCGCAGTCGGCGACGGCGTCAACGAAATGAAGATTG
ATGTGGGGCCGGGATATCGGGCCTACTTCGTGCGACGCGAAAAAATTATCGTCGTGGTGTTGTGCGGCGGGGACAAGTCG
ACGCAGAAGAAGGACATCAAGCTAGCGAAGCAAATCGCTGGCGAACTGGAGGATTGA

Antitoxin

Protein

• Length: 119 a.a.
• Molecular weight: 12527.38 Da
• Isoelectric Point: 10.2187

>AT6106 WP_009971793.1 NC_006351:1437663-1438019 [Burkholderia pseudomallei K96243]
MKISELAEFDGSKYLKDEATIRHYLAQAFEDGNPRLIQAALGNVAKARGMTALARESGVKREALYRALSEGGNAEFATIM
KVVGALGLHLTVAPAEPAPVPAPATTRARSRVRTAAHA

Nucleotide

• Length: 357 bp

>AT6106 NC_006351:1437663-1438019 [Burkholderia pseudomallei K96243]
ATGAAAATCAGCGAACTGGCCGAGTTCGACGGCTCGAAGTACCTGAAGGACGAGGCAACGATTCGTCACTACCTGGCGCA
AGCGTTCGAGGATGGAAACCCGCGGCTGATTCAAGCCGCGCTCGGGAACGTCGCGAAAGCGCGCGGCATGACAGCGCTCG
CGCGCGAGTCCGGCGTGAAGCGTGAAGCGCTCTATCGCGCGCTGTCGGAAGGTGGGAACGCGGAATTCGCAACGATCATG
AAAGTCGTGGGCGCGTTGGGGCTGCACCTGACCGTTGCGCCGGCCGAACCTGCGCCGGTGCCCGCGCCGGCAACAACGCG
TGCACGCTCGCGCGTTCGCACGGCTGCGCACGCGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Toxin

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
T6105	Burkholderia pseudomallei K96243	98.98	100	0.99
T10097	Pseudomonas aeruginosa	46.316	96.939	0.449
T10169	Acinetobacter baumannii	27.778	100	0.306

Multiple sequence alignment

Antitoxin

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
AT6105	Burkholderia pseudomallei K96243	99.153	100	0.992
AT10097	Pseudomonas aeruginosa	37.778	93.75	0.354

Multiple sequence alignment

References

(1) Aaron Butt et al. (2013) Identification of type II toxin-antitoxin modules in Burkholderia pseudomallei. FEMS Microbiology Letters 338(1):86-94. [PubMed:23082999]


 experimental literature