Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | PumAB/- |
| Location | 110952..111538 | Replicon | plasmid pUM505 |
| Accession | NC_016138 | ||
| Organism | Pseudomonas aeruginosa | ||
Toxin (Protein)
| Gene name | PumA | Uniprot ID | G8CP73 |
| Locus tag | HS786_RS00600 | Protein ID | WP_003120987.1 |
| Coordinates | 110952..111251 (+) | Length | 100 a.a. |
Antitoxin (Protein)
| Gene name | PumB | Uniprot ID | - |
| Locus tag | HS786_RS00605 | Protein ID | WP_003448662.1 |
| Coordinates | 111248..111538 (+) | Length | 97 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| HS786_RS00580 (D703_p4119) | 106065..106598 | - | 534 | WP_023125174.1 | type IV pilus biogenesis protein PilP | - |
| HS786_RS00585 (D703_p4120) | 106588..107913 | - | 1326 | WP_023125173.1 | type 4b pilus protein PilO2 | - |
| HS786_RS00590 (D703_p4121) | 107917..109626 | - | 1710 | WP_023125172.1 | PilN family type IVB pilus formation outer membrane protein | - |
| HS786_RS00595 (D703_p4122) | 109626..110750 | - | 1125 | WP_023103776.1 | TcpQ domain-containing protein | - |
| HS786_RS00600 (D703_p4123) | 110952..111251 | + | 300 | WP_003120987.1 | type II toxin-antitoxin system RelE/ParE family toxin | Toxin |
| HS786_RS00605 (D703_p4124) | 111248..111538 | + | 291 | WP_003448662.1 | putative addiction module antidote protein | Antitoxin |
| HS786_RS00610 (D703_p4125) | 111621..111953 | - | 333 | WP_031640358.1 | hypothetical protein | - |
| HS786_RS00615 (D703_p4127) | 112094..114070 | - | 1977 | WP_023125171.1 | DEAD/DEAH box helicase | - |
| HS786_RS00620 (D703_p4128) | 114067..115962 | - | 1896 | WP_031640357.1 | hypothetical protein | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Non-Mobilizable plasmid | - | - | 1..123322 | 123322 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 100 a.a. Molecular weight: 11133.88 Da Isoelectric Point: 10.4495
>T10097 WP_003120987.1 NC_016138:110952-111251 [Pseudomonas aeruginosa]
MVEVKQTATFMAWESKLKDRRAKAVIAARIFRLANGLPGDVSPVGQGVSELRIHYGPGYRVYFQQRGTEIVILLCGGDKS
SQARDIEMAKRLANEWRPQ
MVEVKQTATFMAWESKLKDRRAKAVIAARIFRLANGLPGDVSPVGQGVSELRIHYGPGYRVYFQQRGTEIVILLCGGDKS
SQARDIEMAKRLANEWRPQ
Download Length: 300 bp
>T10097 NC_016138:110952-111251 [Pseudomonas aeruginosa]
ATGGTTGAAGTCAAACAAACCGCCACTTTCATGGCATGGGAAAGCAAGCTAAAGGATCGGCGGGCTAAGGCCGTGATCGC
CGCCCGTATCTTCCGATTGGCGAATGGACTACCCGGCGATGTGTCACCAGTCGGCCAGGGTGTGAGCGAGTTGAGGATTC
ACTATGGCCCAGGCTATCGGGTGTACTTCCAGCAGCGCGGGACGGAAATCGTCATTCTGCTATGTGGTGGAGACAAGAGC
AGCCAGGCCAGGGATATAGAAATGGCGAAACGCCTGGCAAATGAGTGGAGGCCGCAATGA
ATGGTTGAAGTCAAACAAACCGCCACTTTCATGGCATGGGAAAGCAAGCTAAAGGATCGGCGGGCTAAGGCCGTGATCGC
CGCCCGTATCTTCCGATTGGCGAATGGACTACCCGGCGATGTGTCACCAGTCGGCCAGGGTGTGAGCGAGTTGAGGATTC
ACTATGGCCCAGGCTATCGGGTGTACTTCCAGCAGCGCGGGACGGAAATCGTCATTCTGCTATGTGGTGGAGACAAGAGC
AGCCAGGCCAGGGATATAGAAATGGCGAAACGCCTGGCAAATGAGTGGAGGCCGCAATGA
Antitoxin
Download Length: 97 a.a. Molecular weight: 10394.91 Da Isoelectric Point: 4.7527
>AT10097 WP_003448662.1 NC_016138:111248-111538 [Pseudomonas aeruginosa]
MTEQIYDYDPAQALDSPEAMALFIADALDTGDTAYIAKAMGVVARAKGMTELAKETGLAREQLYKSFSERGNPTLKTMLA
VMRALGVDLTARPHAQ
MTEQIYDYDPAQALDSPEAMALFIADALDTGDTAYIAKAMGVVARAKGMTELAKETGLAREQLYKSFSERGNPTLKTMLA
VMRALGVDLTARPHAQ
Download Length: 291 bp
>AT10097 NC_016138:111248-111538 [Pseudomonas aeruginosa]
ATGACCGAGCAAATTTATGATTATGACCCCGCGCAAGCGCTGGACAGCCCGGAGGCCATGGCTCTGTTCATCGCAGACGC
TTTGGATACTGGAGACACCGCCTATATCGCTAAGGCTATGGGAGTCGTGGCCCGCGCCAAGGGGATGACGGAGTTGGCCA
AGGAAACGGGTTTGGCCAGGGAGCAGCTTTACAAATCGTTTAGTGAGCGCGGGAACCCTACATTGAAAACCATGCTGGCA
GTAATGCGAGCTCTGGGAGTTGATCTGACCGCCCGCCCGCATGCTCAGTGA
ATGACCGAGCAAATTTATGATTATGACCCCGCGCAAGCGCTGGACAGCCCGGAGGCCATGGCTCTGTTCATCGCAGACGC
TTTGGATACTGGAGACACCGCCTATATCGCTAAGGCTATGGGAGTCGTGGCCCGCGCCAAGGGGATGACGGAGTTGGCCA
AGGAAACGGGTTTGGCCAGGGAGCAGCTTTACAAATCGTTTAGTGAGCGCGGGAACCCTACATTGAAAACCATGCTGGCA
GTAATGCGAGCTCTGGGAGTTGATCTGACCGCCCGCCCGCATGCTCAGTGA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|---|---|---|---|
| T6105 | Burkholderia pseudomallei K96243 |
47.368 |
96.939 |
0.459 |
| T6106 | Burkholderia pseudomallei K96243 |
46.316 |
96.939 |
0.449 |
Multiple sequence alignment
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|---|---|---|---|
| AT6105 | Burkholderia pseudomallei K96243 |
38.889 |
93.75 |
0.365 |
| AT6106 | Burkholderia pseudomallei K96243 |
37.778 |
93.75 |
0.354 |
Multiple sequence alignment
References
(1) Karen C Hernández-Ramírez et al. (2020) Virulence Conferred by PumA Toxin from the Plasmid-Encoded PumAB Toxin-Antitoxin System is Regulated by Quorum System. Current Microbiology 77(9):2535-2543. [PubMed:32556478]
(2) K C Hernández-Ramírez et al. (2017) Plasmid pUM505 encodes a Toxin-Antitoxin system conferring plasmid stability and increased Pseudomonas aeruginosa virulence. Microbial Pathogenesis 112:259-268. [PubMed:28970172]