Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | relBE/Xre-RelE |
| Location | 178503..179185 | Replicon | chromosome |
| Accession | NC_006350 | ||
| Organism | Burkholderia pseudomallei K96243 | ||
Toxin (Protein)
| Gene name | relE | Uniprot ID | A0A0F6LEW6 |
| Locus tag | BPS_RS00910 | Protein ID | WP_006027262.1 |
| Coordinates | 178862..179158 (-) | Length | 99 a.a. |
Antitoxin (Protein)
| Gene name | relB | Uniprot ID | Q63YL3 |
| Locus tag | BPS_RS00905 | Protein ID | WP_004202809.1 |
| Coordinates | 178503..178859 (-) | Length | 119 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| BPS_RS00885 (BPSL0170) | 173641..174651 | - | 1011 | WP_011204764.1 | phage major capsid protein, P2 family | - |
| BPS_RS00890 (BPSL0171) | 174685..175494 | - | 810 | WP_004532870.1 | GPO family capsid scaffolding protein | - |
| BPS_RS00895 (BPSL0172) | 175638..177407 | + | 1770 | WP_011204765.1 | terminase ATPase subunit family protein | - |
| BPS_RS00900 (BPSL0173) | 177404..178459 | + | 1056 | WP_011204766.1 | phage portal protein | - |
| BPS_RS00905 (BPSL0174) | 178503..178859 | - | 357 | WP_004202809.1 | putative addiction module antidote protein | Antitoxin |
| BPS_RS00910 (BPSL0175) | 178862..179158 | - | 297 | WP_006027262.1 | type II toxin-antitoxin system RelE/ParE family toxin | Toxin |
| BPS_RS00915 (BPSL0176) | 179988..180551 | - | 564 | WP_038782825.1 | hypothetical protein | - |
| BPS_RS00920 | 180548..180904 | - | 357 | WP_162486661.1 | hypothetical protein | - |
| BPS_RS00925 (BPSL0177) | 182072..182806 | + | 735 | WP_004190026.1 | 7-cyano-7-deazaguanine synthase QueC | - |
| BPS_RS00930 (BPSL0178) | 182900..183532 | + | 633 | WP_004189241.1 | 7-carboxy-7-deazaguanine synthase | - |
| BPS_RS00935 (BPSL0179) | 183552..184004 | + | 453 | WP_004189793.1 | 6-carboxytetrahydropterin synthase QueD | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| inside | Prophage | - | - | 146684..183532 | 36848 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 99 a.a. Molecular weight: 10844.65 Da Isoelectric Point: 10.1723
>T6105 WP_006027262.1 NC_006350:c179158-178862 [Burkholderia pseudomallei K96243]
MFKVLTTPQFDKWLDGLRDPVGSAAINLRIERAKLGNLGQWRAVGDGVNEMKIDVGPGYRAYFVRRGKIIVVVLCGGDKS
TQKKDIKLAKQIAGELED
MFKVLTTPQFDKWLDGLRDPVGSAAINLRIERAKLGNLGQWRAVGDGVNEMKIDVGPGYRAYFVRRGKIIVVVLCGGDKS
TQKKDIKLAKQIAGELED
Download Length: 297 bp
>T6105 NC_006350:c179158-178862 [Burkholderia pseudomallei K96243]
ATGTTCAAAGTTCTGACGACCCCCCAGTTTGACAAATGGCTTGACGGGCTTCGCGATCCGGTCGGTAGCGCGGCAATCAA
CCTGCGCATCGAGCGGGCGAAGCTTGGCAATCTCGGCCAGTGGCGCGCAGTCGGCGACGGCGTCAACGAAATGAAGATTG
ATGTGGGGCCGGGATATCGGGCCTATTTCGTGCGGCGCGGAAAAATTATCGTCGTGGTGTTGTGCGGCGGGGACAAGTCG
ACGCAGAAGAAGGACATCAAGCTAGCGAAGCAAATCGCTGGCGAACTGGAGGATTGA
ATGTTCAAAGTTCTGACGACCCCCCAGTTTGACAAATGGCTTGACGGGCTTCGCGATCCGGTCGGTAGCGCGGCAATCAA
CCTGCGCATCGAGCGGGCGAAGCTTGGCAATCTCGGCCAGTGGCGCGCAGTCGGCGACGGCGTCAACGAAATGAAGATTG
ATGTGGGGCCGGGATATCGGGCCTATTTCGTGCGGCGCGGAAAAATTATCGTCGTGGTGTTGTGCGGCGGGGACAAGTCG
ACGCAGAAGAAGGACATCAAGCTAGCGAAGCAAATCGCTGGCGAACTGGAGGATTGA
Antitoxin
Download Length: 119 a.a. Molecular weight: 12585.41 Da Isoelectric Point: 9.9470
>AT6105 WP_004202809.1 NC_006350:c178859-178503 [Burkholderia pseudomallei K96243]
MKISELAEFDGSKYLKDEETIRHYLAQAFEDGNPRLIQAALGNVAKARGMTALARESGVKREALYRALSEGGNAEFATIM
KVVGALGLHLTVAPAEPAPVPAPATTRARSRVRTAAHA
MKISELAEFDGSKYLKDEETIRHYLAQAFEDGNPRLIQAALGNVAKARGMTALARESGVKREALYRALSEGGNAEFATIM
KVVGALGLHLTVAPAEPAPVPAPATTRARSRVRTAAHA
Download Length: 357 bp
>AT6105 NC_006350:c178859-178503 [Burkholderia pseudomallei K96243]
ATGAAAATCAGCGAACTGGCCGAGTTCGACGGCTCGAAGTACCTGAAGGACGAGGAAACGATTCGTCACTACCTGGCGCA
AGCGTTCGAGGATGGAAACCCGCGGCTGATTCAAGCCGCGCTCGGGAACGTCGCGAAAGCGCGCGGCATGACAGCGCTCG
CGCGCGAGTCCGGCGTGAAGCGTGAAGCGCTCTATCGCGCGCTGTCGGAAGGTGGGAACGCGGAATTCGCAACGATCATG
AAAGTCGTGGGCGCGTTGGGGCTGCACCTGACCGTTGCGCCGGCCGAACCTGCGCCGGTGCCCGCGCCGGCAACAACGCG
TGCACGCTCGCGCGTTCGCACGGCTGCGCACGCGTAA
ATGAAAATCAGCGAACTGGCCGAGTTCGACGGCTCGAAGTACCTGAAGGACGAGGAAACGATTCGTCACTACCTGGCGCA
AGCGTTCGAGGATGGAAACCCGCGGCTGATTCAAGCCGCGCTCGGGAACGTCGCGAAAGCGCGCGGCATGACAGCGCTCG
CGCGCGAGTCCGGCGTGAAGCGTGAAGCGCTCTATCGCGCGCTGTCGGAAGGTGGGAACGCGGAATTCGCAACGATCATG
AAAGTCGTGGGCGCGTTGGGGCTGCACCTGACCGTTGCGCCGGCCGAACCTGCGCCGGTGCCCGCGCCGGCAACAACGCG
TGCACGCTCGCGCGTTCGCACGGCTGCGCACGCGTAA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|---|---|---|---|
| T6106 | Burkholderia pseudomallei K96243 |
98.98 |
100 |
0.99 |
| T10097 | Pseudomonas aeruginosa |
47.368 |
96.939 |
0.459 |
| T10169 | Acinetobacter baumannii |
27.778 |
100 |
0.306 |
Multiple sequence alignment
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|---|---|---|---|
| AT6106 | Burkholderia pseudomallei K96243 |
99.153 |
100 |
0.992 |
| AT10097 | Pseudomonas aeruginosa |
38.889 |
93.75 |
0.365 |
Multiple sequence alignment
Structures
Toxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A0F6LEW6 |
Antitoxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A3N4G1D3 |
References
(1) Aaron Butt et al. (2013) Identification of type II toxin-antitoxin modules in Burkholderia pseudomallei. FEMS Microbiology Letters 338(1):86-94. [PubMed:23082999]