Detailed information of TA system TA010063

Experimentally validated

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/-
• Location: 14747..15404
• Replicon: plasmid pEc158
• Accession: NZ_KY887596
• Organism: Escherichia coli strain Ec158

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U3PDC3
• Locus tag: HTM98_RS00130
• Protein ID: WP_000270043.1
• Coordinates: 15054..15404 (-)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: HTM98_RS00125
• Protein ID: WP_000124640.1
• Coordinates: 14747..15049 (-)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HTM98_RS00075	10372..10800	+	429	WP_000591074.1	hypothetical protein	-
HTM98_RS00080	10857..11216	+	360	WP_000422768.1	hypothetical protein	-
HTM98_RS00085	11216..11662	+	447	WP_000919345.1	Fe3+-siderophore ABC transporter permease	-
HTM98_RS00090	11659..12177	+	519	WP_000210756.1	nitrite reductase	-
HTM98_RS00095	12177..12407	+	231	WP_000972663.1	hypothetical protein	-
HTM98_RS00100	12394..13251	+	858	WP_001167032.1	hypothetical protein	-
HTM98_RS00105	13277..13465	+	189	WP_001270411.1	hypothetical protein	-
HTM98_RS00110	13482..14009	+	528	WP_001236377.1	thermonuclease family protein	-
HTM98_RS00115	14067..14339	+	273	WP_001043047.1	HU family DNA-binding protein	-
HTM98_RS00120	14427..14720	+	294	WP_001239997.1	chromosome segregation protein ParM	-
HTM98_RS00125	14747..15049	-	303	WP_000124640.1	XRE family transcriptional regulator	Antitoxin
HTM98_RS00130	15054..15404	-	351	WP_000270043.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HTM98_RS00135	15567..16115	+	549	WP_001061574.1	transcriptional regulator	-
HTM98_RS00140	16289..16456	+	168	WP_015059388.1	hypothetical protein	-
HTM98_RS00145	16456..16650	+	195	WP_000343597.1	hypothetical protein	-
HTM98_RS00150	16661..17032	+	372	WP_000516916.1	hypothetical protein	-
HTM98_RS00155	17025..17495	+	471	WP_001281821.1	hypothetical protein	-
HTM98_RS00160	17510..17845	-	336	WP_000683476.1	hypothetical protein	-
HTM98_RS00165	17942..18430	+	489	WP_001273096.1	DUF1643 domain-containing protein	-
HTM98_RS00170	18433..18930	+	498	WP_000062185.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	Similar MGE(s) Similar MGE(s) is defined as other TA-related MGE(s) with Mash distance < 0.01 to this MGE. Detail Genome accession Organism MGE type Related TA Coordinates Mash distance View NZ_CP019053 Escherichia coli strain CRE1540 Conjugative plasmid TA071638 1..155802 0.01 View NZ_CP049113 Escherichia coli strain WF5-5-T2 Conjugative plasmid TA150580 1..183610 0.007 View NZ_CP054408 Escherichia coli strain KCJ3K291 Conjugative plasmid TA159690 1..147764 0.009 View NZ_CP060954 Escherichia coli strain EC8 Conjugative plasmid TA171743 1..168756 0.009 View NZ_CP066808 Escherichia coli strain TCM3e1 Conjugative plasmid TA185097 1..191962 0.01	inside	Conjugative plasmid	blaIMP-4 / catB3 / qacE / sul1 / mph(A) / aac(3)-IId / dfrA35 / blaTEM-1B	-	1..165169	165169

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Cro/C1-type helix-turn-helix domain (33-79)

Antitoxin

• Cro/C1-type helix-turn-helix domain (33-79)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 11006.73 Da
• Isoelectric Point: 7.2036

>T10063 WP_000270043.1 NZ_KY887596:c15404-15054 [Escherichia coli]
MWVIETTDTFDEWFDALDDTDRANVLASMMVLRDRGPMLSRPYADTVNGSSYSNMKELRVQSKGDPIRAFFAFDPKRKGI
LLCAGNKTGDEKRFYEVMIPIADREFAAHLDKLKKE

Nucleotide

• Length: 351 bp

>T10063 NZ_KY887596:c15404-15054 [Escherichia coli]
ATGTGGGTCATCGAGACAACCGACACCTTTGATGAGTGGTTCGATGCTCTGGATGATACCGATAGAGCAAACGTGCTGGC
TTCGATGATGGTGCTGCGAGATAGAGGCCCCATGCTGTCGAGGCCATACGCGGATACTGTTAACGGTTCATCCTACAGCA
ACATGAAAGAGCTTCGGGTCCAAAGCAAAGGAGATCCTATCAGAGCGTTCTTTGCGTTCGATCCAAAGCGTAAGGGGATT
CTTCTCTGCGCCGGTAACAAGACCGGGGACGAGAAAAGGTTTTATGAAGTAATGATCCCAATTGCAGACCGTGAGTTTGC
GGCGCACTTGGATAAATTGAAGAAGGAGTGA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11006.73 Da
• Isoelectric Point: 7.2036

>AT10063 WP_000124640.1 NZ_KY887596:c15049-14747 [Escherichia coli]
MARTLDQMLATEKPEVVAKAQKAATEMLLNIHLAELRDRMNLTQGEIAASLGVRQPTVSEMEKPGRDLKLSSIKRYVEAS
GGKLRLDVELPDGTHYGFAV

Nucleotide

• Length: 303 bp

>AT10063 NZ_KY887596:c15049-14747 [Escherichia coli]
ATGGCAAGAACTCTTGACCAAATGCTGGCAACAGAAAAGCCTGAGGTTGTTGCCAAAGCACAGAAAGCGGCCACTGAGAT
GTTACTGAACATCCACTTGGCAGAGCTTCGTGATCGCATGAACCTTACCCAGGGGGAAATTGCTGCATCTCTGGGTGTGA
GACAGCCGACAGTCTCCGAGATGGAAAAACCTGGACGAGATCTCAAGCTGTCGTCCATTAAGCGGTACGTTGAGGCCTCC
GGTGGCAAGCTACGCCTCGATGTTGAACTTCCGGACGGCACCCACTACGGCTTTGCCGTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Antitoxin

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
AT10169	Acinetobacter baumannii	51	100	0.51
AT10050	Mycobacterium tuberculosis H37Rv	33.333	99	0.33

Multiple sequence alignment

Structures

Toxin

• AlphaFold DB: A0A7G8AFB9

References

(1) Qin Qi et al. (2021) The higBA-Type Toxin-Antitoxin System in IncC Plasmids Is a Mobilizable Ciprofloxacin-Inducible System. MSphere 6(3):e0042421. [PubMed:34077263]


 experimental literature