Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | higBA (relBE)/COG4683-HTH_37 |
| Location | 71432..72089 | Replicon | plasmid pWF5_NDM |
| Accession | NZ_CP049113 | ||
| Organism | Escherichia coli strain WF5-5-T2 | ||
Toxin (Protein)
| Gene name | higB | Uniprot ID | U3PDC3 |
| Locus tag | G6R06_RS22750 | Protein ID | WP_000270043.1 |
| Coordinates | 71739..72089 (-) | Length | 117 a.a. |
Antitoxin (Protein)
| Gene name | higA | Uniprot ID | - |
| Locus tag | G6R06_RS22745 | Protein ID | WP_000124640.1 |
| Coordinates | 71432..71734 (-) | Length | 101 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| G6R06_RS22695 (G6R06_22500) | 67057..67485 | + | 429 | WP_072713905.1 | hypothetical protein | - |
| G6R06_RS22700 (G6R06_22505) | 67542..67901 | + | 360 | WP_000422768.1 | hypothetical protein | - |
| G6R06_RS22705 (G6R06_22510) | 67901..68347 | + | 447 | WP_000919345.1 | Fe3+-siderophore ABC transporter permease | - |
| G6R06_RS22710 (G6R06_22515) | 68344..68862 | + | 519 | WP_000210756.1 | nitrite reductase | - |
| G6R06_RS22715 (G6R06_22520) | 68862..69092 | + | 231 | WP_000972663.1 | hypothetical protein | - |
| G6R06_RS22720 (G6R06_22525) | 69079..69936 | + | 858 | WP_001167032.1 | hypothetical protein | - |
| G6R06_RS22725 (G6R06_22530) | 69962..70150 | + | 189 | WP_001270411.1 | hypothetical protein | - |
| G6R06_RS22730 (G6R06_22535) | 70167..70694 | + | 528 | WP_001236377.1 | thermonuclease family protein | - |
| G6R06_RS22735 (G6R06_22540) | 70752..71024 | + | 273 | WP_001043047.1 | HU family DNA-binding protein | - |
| G6R06_RS22740 (G6R06_22545) | 71112..71405 | + | 294 | WP_001239997.1 | chromosome segregation protein ParM | - |
| G6R06_RS22745 (G6R06_22550) | 71432..71734 | - | 303 | WP_000124640.1 | XRE family transcriptional regulator | Antitoxin |
| G6R06_RS22750 (G6R06_22555) | 71739..72089 | - | 351 | WP_000270043.1 | type II toxin-antitoxin system RelE/ParE family toxin | Toxin |
| G6R06_RS22755 (G6R06_22560) | 72252..72800 | + | 549 | WP_001061574.1 | transcriptional regulator | - |
| G6R06_RS22760 (G6R06_22570) | 73141..73335 | + | 195 | WP_000343597.1 | hypothetical protein | - |
| G6R06_RS22765 (G6R06_22575) | 73346..73717 | + | 372 | WP_000516916.1 | hypothetical protein | - |
| G6R06_RS22770 (G6R06_22580) | 73710..74180 | + | 471 | WP_001281821.1 | hypothetical protein | - |
| G6R06_RS22775 (G6R06_22585) | 74195..74530 | - | 336 | WP_000683476.1 | hypothetical protein | - |
| G6R06_RS22780 (G6R06_22590) | 74627..75115 | + | 489 | WP_001273096.1 | DUF1643 domain-containing protein | - |
| G6R06_RS22785 (G6R06_22595) | 75118..75615 | + | 498 | WP_000062185.1 | hypothetical protein | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| inside | Conjugative plasmid | blaNDM-4 / sul1 / qacE / aadA2 / dfrA12 / aac(3)-IId / blaTEM-1B / mph(A) | - | 1..183610 | 183610 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 117 a.a. Molecular weight: 13328.21 Da Isoelectric Point: 5.2421
>T150580 WP_000270043.1 NZ_CP049113:c72089-71739 [Escherichia coli]
MWVIETTDTFDEWFDALDDTDRANVLASMMVLRDRGPMLSRPYADTVNGSSYSNMKELRVQSKGDPIRAFFAFDPKRKGI
LLCAGNKTGDEKRFYEVMIPIADREFAAHLDKLKKE
MWVIETTDTFDEWFDALDDTDRANVLASMMVLRDRGPMLSRPYADTVNGSSYSNMKELRVQSKGDPIRAFFAFDPKRKGI
LLCAGNKTGDEKRFYEVMIPIADREFAAHLDKLKKE
Download Length: 351 bp
>T150580 NZ_CP049113:c72089-71739 [Escherichia coli]
ATGTGGGTCATCGAGACAACCGACACCTTTGATGAGTGGTTCGATGCTCTGGATGATACCGATAGAGCAAACGTGCTGGC
TTCGATGATGGTGCTGCGAGATAGAGGCCCCATGCTGTCGAGGCCATACGCGGATACTGTTAACGGTTCATCCTACAGCA
ACATGAAAGAGCTTCGGGTCCAAAGCAAAGGAGATCCTATCAGAGCGTTCTTTGCGTTCGATCCAAAGCGTAAGGGGATT
CTTCTCTGCGCCGGTAACAAGACCGGGGACGAGAAAAGGTTTTATGAAGTAATGATCCCAATTGCAGACCGTGAGTTTGC
GGCGCACTTGGATAAATTGAAGAAGGAGTGA
ATGTGGGTCATCGAGACAACCGACACCTTTGATGAGTGGTTCGATGCTCTGGATGATACCGATAGAGCAAACGTGCTGGC
TTCGATGATGGTGCTGCGAGATAGAGGCCCCATGCTGTCGAGGCCATACGCGGATACTGTTAACGGTTCATCCTACAGCA
ACATGAAAGAGCTTCGGGTCCAAAGCAAAGGAGATCCTATCAGAGCGTTCTTTGCGTTCGATCCAAAGCGTAAGGGGATT
CTTCTCTGCGCCGGTAACAAGACCGGGGACGAGAAAAGGTTTTATGAAGTAATGATCCCAATTGCAGACCGTGAGTTTGC
GGCGCACTTGGATAAATTGAAGAAGGAGTGA
Antitoxin
Download Length: 101 a.a. Molecular weight: 11006.73 Da Isoelectric Point: 7.2036
>AT150580 WP_000124640.1 NZ_CP049113:c71734-71432 [Escherichia coli]
MARTLDQMLATEKPEVVAKAQKAATEMLLNIHLAELRDRMNLTQGEIAASLGVRQPTVSEMEKPGRDLKLSSIKRYVEAS
GGKLRLDVELPDGTHYGFAV
MARTLDQMLATEKPEVVAKAQKAATEMLLNIHLAELRDRMNLTQGEIAASLGVRQPTVSEMEKPGRDLKLSSIKRYVEAS
GGKLRLDVELPDGTHYGFAV
Download Length: 303 bp
>AT150580 NZ_CP049113:c71734-71432 [Escherichia coli]
ATGGCAAGAACTCTTGACCAAATGCTGGCAACAGAAAAGCCTGAGGTTGTTGCCAAAGCACAGAAAGCGGCCACTGAGAT
GTTACTGAACATCCACTTGGCAGAGCTTCGTGATCGCATGAACCTTACCCAGGGGGAAATTGCTGCATCTCTGGGTGTGA
GACAGCCGACAGTCTCCGAGATGGAAAAACCTGGACGAGATCTCAAGCTGTCGTCCATTAAGCGGTACGTTGAGGCCTCC
GGTGGCAAGCTACGCCTCGATGTTGAACTTCCGGACGGCACCCACTACGGCTTTGCCGTTTAA
ATGGCAAGAACTCTTGACCAAATGCTGGCAACAGAAAAGCCTGAGGTTGTTGCCAAAGCACAGAAAGCGGCCACTGAGAT
GTTACTGAACATCCACTTGGCAGAGCTTCGTGATCGCATGAACCTTACCCAGGGGGAAATTGCTGCATCTCTGGGTGTGA
GACAGCCGACAGTCTCCGAGATGGAAAAACCTGGACGAGATCTCAAGCTGTCGTCCATTAAGCGGTACGTTGAGGCCTCC
GGTGGCAAGCTACGCCTCGATGTTGAACTTCCGGACGGCACCCACTACGGCTTTGCCGTTTAA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|