159690

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 69018..69675 Replicon plasmid pKC148K
Accession NZ_CP054408
Organism Escherichia coli strain KCJ3K291

Toxin (Protein)


Gene name higB Uniprot ID U3PDC3
Locus tag FR957_RS24610 Protein ID WP_000270043.1
Coordinates 69325..69675 (-) Length 117 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag FR957_RS24605 Protein ID WP_000124640.1
Coordinates 69018..69320 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FR957_RS24560 (FR957_024475) 64643..65071 + 429 WP_000591074.1 hypothetical protein -
FR957_RS24565 (FR957_024480) 65128..65487 + 360 WP_000422768.1 hypothetical protein -
FR957_RS24570 (FR957_024485) 65487..65933 + 447 WP_000919345.1 Fe3+-siderophore ABC transporter permease -
FR957_RS24575 (FR957_024490) 65930..66448 + 519 WP_000210756.1 nitrite reductase -
FR957_RS24580 (FR957_024495) 66448..66678 + 231 WP_000972663.1 hypothetical protein -
FR957_RS24585 (FR957_024500) 66665..67522 + 858 WP_001167032.1 hypothetical protein -
FR957_RS24590 (FR957_024505) 67753..68280 + 528 WP_001236377.1 thermonuclease family protein -
FR957_RS24595 (FR957_024510) 68338..68610 + 273 WP_001043047.1 HU family DNA-binding protein -
FR957_RS24600 (FR957_024515) 68698..68991 + 294 WP_001239997.1 chromosome segregation protein ParM -
FR957_RS24605 (FR957_024520) 69018..69320 - 303 WP_000124640.1 XRE family transcriptional regulator Antitoxin
FR957_RS24610 (FR957_024525) 69325..69675 - 351 WP_000270043.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
FR957_RS24615 (FR957_024530) 69838..70386 + 549 WP_001061574.1 transcriptional regulator -
FR957_RS24620 (FR957_024535) 70727..70921 + 195 WP_000343597.1 hypothetical protein -
FR957_RS24625 (FR957_024540) 70932..71303 + 372 WP_000516916.1 hypothetical protein -
FR957_RS24630 (FR957_024545) 71296..71766 + 471 WP_001281821.1 hypothetical protein -
FR957_RS24635 (FR957_024550) 71781..72116 - 336 WP_000683476.1 hypothetical protein -
FR957_RS24640 (FR957_024555) 72213..72701 + 489 WP_001273096.1 DUF1643 domain-containing protein -
FR957_RS24645 (FR957_024560) 72704..73201 + 498 WP_000062185.1 hypothetical protein -
FR957_RS24650 (FR957_024565) 73416..74120 - 705 WP_001067855.1 IS6-like element IS26 family transposase -
FR957_RS24655 (FR957_024570) 74183..74476 - 294 Protein_95 tunicamycin resistance protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Conjugative plasmid aac(3)-IId / mph(A) / sul1 / qacE / ARR-3 / blaNDM-1 / aadA16 / dfrA27 / aac(6')-Ib-cr / aph(3')-Ia / aph(6)-Id / aph(3'')-Ib / sul2 - 1..147764 147764


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-106)

Antitoxin

Cro/C1-type helix-turn-helix domain

(33-79)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 13328.21 Da        Isoelectric Point: 5.2421

>T159690 WP_000270043.1 NZ_CP054408:c69675-69325 [Escherichia coli]
MWVIETTDTFDEWFDALDDTDRANVLASMMVLRDRGPMLSRPYADTVNGSSYSNMKELRVQSKGDPIRAFFAFDPKRKGI
LLCAGNKTGDEKRFYEVMIPIADREFAAHLDKLKKE

Download         Length: 351 bp

>T159690 NZ_CP070111:1012444-1013766 [Escherichia coli]
ATGCCTAAACTAGTCACTTGGATGAACAACCAGCGGGTAGGCGAGTTAACGAAGTTAGCCAACGGCGCGCACACCTTTAA
GTATGCACCTGAGTGGTTAGCCAGCCGTTATGCCAGACCGTTGTCACTTTCGCTGCCATTGCAGAGGAGGAATATCACCT
CTGATGCCGTATTTAACTTCTTCGATAACCTGTTACCCGATAGCCCGATTGTACGTGACCGGATCGTTAAACGTTATCAT
GCTAAATCCAGACAACCGTTTGATTTATTGTCAGAAATAGGGCGAGACAGCGTTGGTGCCGTGACGTTAATCCCCGAAGA
TGAAACCGTAATGTGTCCGATAATGGCATGGGAAAAGCTTACTGAAGCCAGACTTGAAGAAGTATTAACGGCTTATAAAG
CAGATATCCCGTTAGGCATGATTAGAGAAGAAAATGACTTTCGCATCTCGGTTGCTGGCGCACAGGAGAAGACTGCGCTG
CTCAGAATAGGCAATGACTGGTGCATTCCGAAGGGAATAACGCCAACGACGCACATTATCAAATTGCCGATTGGCGAAAT
CAGACAGCCCAATGCGACGCTCGATCTTAGCCAAAGCGTTGATAATGAGTATTACTGTTTGCTGCTGGCGAAAGAACTCG
GGTTGAATGTTCCGGACGCAGAAATCATTAAAGCGGGAAGGGTGCGCGCGTTAGCGGTCAAACGTTTTGACAGGCGTTGG
AATACTGAGCGAACGGTTTTACTTCGCTTGCCACAGGAGGATATGTGCCAGACATTCGGTTTACCTTCATCGGTGAAATA
TGAATCAGATGGAGGCCCCGGTATCGCGCAAATTATGGCTTTTTTGATGGGGTCCAGCGAGGCGCTGAAAGATCGCTATG
ATTTTATGAAGTTTCAGGTCTTCCAGTGGTTGATTGGCGCAACAGATGGCCATGCAAAAAACTTCTCCGTATTTATTCAG
GCTGGCGGCAGTTATCGGCTCACACCATTTTATGACATCATTTCGGCATTTCCGGTCCTTGGCGGTACGGGAATACACAT
CAGCGATCTCAAACTGGCAATGGGGCTTAACGCATCCAAAGGCAAAAAAACGGCAATCGATAAAATTTATCCGCGACATT
TTCTGGCGACAGCAAAGGTGCTGAAATTCCCGGAAGTGCAGATGCATGAAATCCTGAGTGACTTTGCCAGAATGATTCCG
GCAGCACTGGATAACGTGAAGACTTCATTACCGACAGATTTTCCGGAGAACGTGGTGACGGCAGTTGAAACCAATGTGTT
GAGGTTGCACGGTCGGTTAAGCCGAGAATACGGTATTAAGTAA

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11006.73 Da        Isoelectric Point: 7.2036

>AT159690 WP_000124640.1 NZ_CP054408:c69320-69018 [Escherichia coli]
MARTLDQMLATEKPEVVAKAQKAATEMLLNIHLAELRDRMNLTQGEIAASLGVRQPTVSEMEKPGRDLKLSSIKRYVEAS
GGKLRLDVELPDGTHYGFAV

Download         Length: 303 bp

>AT159690 NZ_CP070111:1012178-1012444 [Escherichia coli]
ATGATGAGCTTTCAGAAGATCTATAGCCCAATGCAATTGGCGAATGCAATGAAACTGGTTCGCCAGCAAAACGGCTGGAC
GCAGAGCGAGTTGGCGAAAAAAATCGGCATTAAGCAGGCGACAATTTCCAATTTCGAAAACAATCCTAACAATACCTCGC
TCACGACATTTTTTAAGATTTTACAGTCGCTTGAACTCTCAATGACGCTATGCGACGCAAAAAATGCCTCGCCAGAAGCA
ACAGAACAGCAAGATCTGGAGTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7G8AFB9

Antitoxin

Source ID Structure

References