Detailed information of TA system TA109195

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): BrnTA/BrnT-BrnA
• Location: 2186..2774
• Replicon: plasmid pAB120
• Accession: NZ_CP031446
• Organism: Acinetobacter baumannii strain MDR-UNC

Toxin (Protein)

• Gene name: brnT
• Uniprot ID: A0A3G6Z3M6
• Locus tag: DYB08_RS20055
• Protein ID: WP_000438826.1
• Coordinates: 2186..2473 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: brnA
• Uniprot ID: N9M5I3
• Locus tag: DYB08_RS20060
• Protein ID: WP_001983304.1
• Coordinates: 2460..2774 (+)
• Length: 105 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DYB08_RS20045	815..1006	+	192	WP_000032259.1	hypothetical protein	-
DYB08_RS20050	1050..1877	-	828	WP_000713530.1	OXA-24 family carbapenem-hydrolyzing class D beta-lactamase OXA-72	-
DYB08_RS20055	2186..2473	+	288	WP_000438826.1	BrnT family toxin	Toxin
DYB08_RS20060	2460..2774	+	315	WP_001983304.1	BrnA antitoxin family protein	Antitoxin
DYB08_RS20065	2902..5313	+	2412	WP_000932953.1	TonB-dependent receptor ZnuD2	-
DYB08_RS20070	5618..6079	+	462	WP_000761346.1	DIP1984 family protein	-
DYB08_RS20075	6405..6614	-	210	WP_000069473.1	hypothetical protein	-
DYB08_RS20080	6607..6909	-	303	WP_001140622.1	XRE family transcriptional regulator	-
DYB08_RS20085	6902..7258	-	357	WP_000269902.1	type II toxin-antitoxin system RelE/ParE family toxin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	Similar MGE(s) Similar MGE(s) is defined as other TA-related MGE(s) with Mash distance < 0.01 to this MGE. Detail Genome accession Organism MGE type Related TA Coordinates Mash distance View NC_019359 Acinetobacter baumannii Non-Mobilizable plasmid TA010169 1..10879 0	inside	Non-Mobilizable plasmid	blaOXA-72	-	1..10879	10879

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (7-88)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (41-96)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11303.72 Da
• Isoelectric Point: 6.0889

>T109195 WP_000438826.1 NZ_CP031446:2186-2473 [Acinetobacter baumannii]
MEQYFEWDEAKNRKNQKKHDISFETASLVFEDPLRISIQDRHTNGEERWQTIGRVKGVLMLLVAHTIFDEDDCEIIRIIS
ARQVTKAERNKYEHG

Nucleotide

• Length: 288 bp

>T109195 NZ_CP031446:2186-2473 [Acinetobacter baumannii]
ATGGAACAGTATTTTGAATGGGATGAGGCTAAGAATCGAAAGAATCAGAAAAAGCATGATATCTCTTTCGAAACTGCAAG
CCTTGTTTTTGAAGATCCATTACGGATCTCAATCCAAGACAGACATACCAATGGTGAAGAACGTTGGCAAACCATTGGAC
GAGTTAAAGGCGTACTAATGTTATTGGTGGCTCACACCATTTTTGATGAAGATGACTGTGAAATCATCCGAATCATTAGT
GCAAGGCAAGTCACTAAAGCGGAGCGAAACAAATATGAGCATGGTTAG

Antitoxin

Protein

• Length: 105 a.a.
• Molecular weight: 12208.84 Da
• Isoelectric Point: 7.2026

>AT109195 WP_001983304.1 NZ_CP031446:2460-2774 [Acinetobacter baumannii]
MSMVRYSHKELNEKFGEKQDAEIQRLLAKGTVPDDQLDLSDIPEITDWSNAVRQNQFYRPVKQQTSIRLDADVLAWFKAQ
GKGYQTRMNEILRDAMLKELKNHQ

Nucleotide

• Length: 315 bp

>AT109195 NZ_CP031446:2460-2774 [Acinetobacter baumannii]
ATGAGCATGGTTAGATACTCACACAAAGAGCTGAATGAGAAATTTGGTGAGAAGCAAGATGCTGAAATTCAACGCTTGCT
TGCTAAAGGCACGGTTCCTGATGATCAGCTAGATCTATCAGATATTCCTGAAATTACAGACTGGAGCAATGCTGTACGCC
AAAATCAATTTTACCGCCCAGTAAAGCAACAAACTTCAATTCGGTTAGATGCTGATGTACTTGCTTGGTTCAAAGCACAA
GGCAAAGGTTATCAAACACGTATGAATGAAATTTTACGAGATGCTATGCTAAAAGAATTAAAAAATCATCAATAA

Structures

Toxin

• AlphaFold DB: A0A3G6Z3M6

Antitoxin

• AlphaFold DB: N9M5I3