206474

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) BrnTA/BrnT-BrnA
Location 3579..4167 Replicon plasmid unnamed1
Accession NZ_CP076740
Organism Acinetobacter baumannii strain X4-584

Toxin (Protein)


Gene name brnT Uniprot ID A0A3G6Z3M6
Locus tag KRO88_RS19170 Protein ID WP_000438826.1
Coordinates 3579..3866 (+) Length 96 a.a.

Antitoxin (Protein)


Gene name brnA Uniprot ID N9M5I3
Locus tag KRO88_RS19175 Protein ID WP_001983304.1
Coordinates 3853..4167 (+) Length 105 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KRO88_RS19150 (KRO88_19035) 579..1529 + 951 WP_001205343.1 replication initiation protein RepM -
KRO88_RS19155 (KRO88_19040) 1522..2097 + 576 WP_001096616.1 plasmid replication DNA-binding protein -
KRO88_RS19160 (KRO88_19045) 2117..2260 + 144 WP_001125246.1 hypothetical protein -
KRO88_RS19165 (KRO88_19050) 2443..3270 - 828 WP_000713530.1 OXA-24 family carbapenem-hydrolyzing class D beta-lactamase OXA-72 -
KRO88_RS19170 (KRO88_19055) 3579..3866 + 288 WP_000438826.1 BrnT family toxin Toxin
KRO88_RS19175 (KRO88_19060) 3853..4167 + 315 WP_001983304.1 BrnA antitoxin family protein Antitoxin
KRO88_RS19180 (KRO88_19065) 4295..6706 + 2412 WP_000932953.1 TonB-dependent receptor ZnuD2 -
KRO88_RS19185 (KRO88_19070) 7011..7472 + 462 WP_000761346.1 DIP1984 family protein -
KRO88_RS19190 (KRO88_19075) 7795..7968 - 174 WP_001282484.1 hypothetical protein -
KRO88_RS19195 (KRO88_19080) 7968..8339 - 372 WP_000504218.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Non-Mobilizable plasmid blaOXA-72 - 1..8954 8954


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(41-96)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11303.72 Da        Isoelectric Point: 6.0889

>T206474 WP_000438826.1 NZ_CP076740:3579-3866 [Acinetobacter baumannii]
MEQYFEWDEAKNRKNQKKHDISFETASLVFEDPLRISIQDRHTNGEERWQTIGRVKGVLMLLVAHTIFDEDDCEIIRIIS
ARQVTKAERNKYEHG

Download         Length: 288 bp

>T206474 NZ_CP102438:2002517-2002619 [Klebsiella pneumoniae]
GCAAGGCGACTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATACA
GGAATCGTATTCGGTCTCTTTTT

Antitoxin


Download         Length: 105 a.a.        Molecular weight: 12208.84 Da        Isoelectric Point: 7.2026

>AT206474 WP_001983304.1 NZ_CP076740:3853-4167 [Acinetobacter baumannii]
MSMVRYSHKELNEKFGEKQDAEIQRLLAKGTVPDDQLDLSDIPEITDWSNAVRQNQFYRPVKQQTSIRLDADVLAWFKAQ
GKGYQTRMNEILRDAMLKELKNHQ

Download         Length: 315 bp

>AT206474 NZ_CP102438:c2002626-2002481 [Klebsiella pneumoniae]
TAAAGATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAG
TTGGCATTAATGCAGGCTAAGTCGCCTTGCACTATAAGAATAGTTTAACGCGTCAGCTTTTCCAGT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A3G6Z3M6

Antitoxin

Source ID Structure
AlphaFold DB N9M5I3

References