65088

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 3546841..3547511 Replicon chromosome
Accession NZ_CP016794
Organism Mycobacterium tuberculosis strain SCAID 320.0

Toxin (Protein)


Gene name higB Uniprot ID O53332
Locus tag BEE65_RS16810 Protein ID WP_003899954.1
Coordinates 3546841..3547185 (+) Length 115 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID G0THF6
Locus tag BEE65_RS16815 Protein ID WP_003899955.1
Coordinates 3547182..3547511 (+) Length 110 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BEE65_RS16780 3542146..3542439 + 294 WP_003416635.1 hypothetical protein -
BEE65_RS16785 3542727..3544016 + 1290 WP_003416640.1 ATP-binding protein -
BEE65_RS16800 3545721..3546155 - 435 WP_003899952.1 type II toxin-antitoxin system VapC family toxin -
BEE65_RS16805 3546158..3546610 - 453 WP_003899953.1 type II toxin-antitoxin system Phd/YefM family antitoxin -
BEE65_RS16810 3546841..3547185 + 345 WP_003899954.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
BEE65_RS16815 3547182..3547511 + 330 WP_003899955.1 XRE family transcriptional regulator Antitoxin
BEE65_RS22250 3547706..3548727 + 1022 Protein_3289 IS3-like element IS987 family transposase -
BEE65_RS22255 3548863..3549944 + 1082 Protein_3290 IS3-like element IS987 family transposase -
BEE65_RS16835 3550171..3550518 + 348 WP_003899956.1 DUF2384 domain-containing protein -
BEE65_RS16840 3550515..3551135 + 621 WP_003899957.1 RES family NAD+ phosphorylase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - - 3544351..3576368 32017
- inside IScluster/Tn - - 3544351..3550067 5716


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-103)

Antitoxin

Cro/C1-type helix-turn-helix domain

(38-91)


Sequences

Toxin        


Download         Length: 115 a.a.        Molecular weight: 12692.50 Da        Isoelectric Point: 5.6920

>T65088 WP_003899954.1 NZ_CP016794:3546841-3547185 [Mycobacterium tuberculosis]
MAVILLPQVERWFFALNRDAMASVTGAIDLLEMEGPTLGRPVVDKVNDSTFHNMKELRPAGTSIRILFAFDPARQAILLL
GGDKAGNWKRWYDNNIPIADQRSENWLASEHGGG

Download         Length: 345 bp

>T65088 NZ_CP016794:3546841-3547185 [Mycobacterium tuberculosis]
TTGGCCGTGATCCTGCTCCCGCAGGTCGAACGGTGGTTCTTCGCGCTCAACAGGGATGCGATGGCCTCGGTCACCGGCGC
CATCGACCTGCTCGAAATGGAGGGGCCGACGTTGGGCCGCCCGGTGGTCGACAAAGTGAACGACTCAACGTTTCACAACA
TGAAGGAGCTGCGCCCCGCCGGCACCAGCATCCGGATCCTGTTCGCCTTCGACCCGGCCCGGCAGGCGATCCTGCTGCTG
GGCGGTGACAAGGCAGGCAACTGGAAACGCTGGTACGACAACAACATTCCAATCGCTGACCAGCGCTCCGAGAACTGGCT
GGCGAGCGAGCACGGAGGTGGATGA

Antitoxin


Download         Length: 110 a.a.        Molecular weight: 11802.46 Da        Isoelectric Point: 7.4051

>AT65088 WP_003899955.1 NZ_CP016794:3547182-3547511 [Mycobacterium tuberculosis]
MTMARNWRDIRADAVAQGRVDLQRAAVAREEMRDAVLAHRLAEIRKALGHARQADVAALMGVSQARVSKLESGDLSHTEL
GTLQAYVAALGGHLRIVAEFGENTVELTA

Download         Length: 330 bp

>AT65088 NZ_CP016794:3547182-3547511 [Mycobacterium tuberculosis]
ATGACCATGGCCCGCAACTGGCGTGACATTCGCGCCGATGCCGTCGCGCAGGGCCGCGTGGATCTGCAGCGGGCGGCCGT
GGCACGCGAGGAGATGCGCGATGCCGTCCTGGCGCACCGCCTGGCCGAGATCCGCAAGGCGCTAGGCCACGCACGTCAGG
CCGACGTCGCGGCGCTGATGGGGGTCTCTCAGGCCCGTGTCTCCAAGCTGGAGAGCGGCGACCTGTCCCACACCGAACTC
GGCACCCTGCAGGCCTACGTTGCCGCCCTGGGCGGGCACCTGCGCATCGTCGCTGAGTTCGGCGAAAATACTGTCGAGCT
GACCGCCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U4FBK2

Antitoxin

Source ID Structure
PDB 6LTY
PDB 6LTZ
AlphaFold DB G0THF6

References