83663

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 3550320..3550990 Replicon chromosome
Accession NZ_CP023605
Organism Mycobacterium tuberculosis strain LE79

Toxin (Protein)


Gene name higB Uniprot ID O53332
Locus tag CP461_RS17415 Protein ID WP_003899954.1
Coordinates 3550320..3550664 (+) Length 115 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID G0THF6
Locus tag CP461_RS17420 Protein ID WP_003899955.1
Coordinates 3550661..3550990 (+) Length 110 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CP461_RS17380 3545393..3546253 + 861 WP_003416628.1 alpha/beta hydrolase -
CP461_RS17385 3546228..3546743 + 516 WP_003899950.1 nitroreductase family deazaflavin-dependent oxidoreductase -
CP461_RS17395 3546983..3547276 + 294 WP_003416635.1 hypothetical protein -
CP461_RS17400 3547564..3548853 + 1290 WP_003416640.1 ATP-binding protein -
CP461_RS17405 3549200..3549634 - 435 WP_003899952.1 type II toxin-antitoxin system VapC family toxin -
CP461_RS17410 3549637..3550089 - 453 WP_003899953.1 type II toxin-antitoxin system Phd/YefM family antitoxin -
CP461_RS17415 3550320..3550664 + 345 WP_003899954.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
CP461_RS17420 3550661..3550990 + 330 WP_003899955.1 XRE family transcriptional regulator Antitoxin
CP461_RS17425 3551227..3552488 + 1262 WP_087902221.1 IS3-like element IS987 family transposase -
CP461_RS17430 3552710..3553971 + 1262 WP_087902221.1 IS3-like element IS987 family transposase -
CP461_RS17435 3554244..3554591 + 348 WP_003899956.1 DUF2384 domain-containing protein -
CP461_RS17440 3554588..3555208 + 621 WP_003899957.1 RES family NAD+ phosphorylase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - - 3547564..3580441 32877


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-103)

Antitoxin

Cro/C1-type helix-turn-helix domain

(38-91)


Sequences

Toxin        


Download         Length: 115 a.a.        Molecular weight: 12692.50 Da        Isoelectric Point: 5.6920

>T83663 WP_003899954.1 NZ_CP023605:3550320-3550664 [Mycobacterium tuberculosis]
MAVILLPQVERWFFALNRDAMASVTGAIDLLEMEGPTLGRPVVDKVNDSTFHNMKELRPAGTSIRILFAFDPARQAILLL
GGDKAGNWKRWYDNNIPIADQRSENWLASEHGGG

Download         Length: 345 bp

>T83663 NZ_CP023605:3550320-3550664 [Mycobacterium tuberculosis]
TTGGCCGTGATCCTGCTCCCGCAGGTCGAACGGTGGTTCTTCGCGCTCAACAGGGATGCGATGGCCTCGGTCACCGGCGC
CATCGACCTGCTCGAAATGGAGGGGCCGACGTTGGGCCGCCCGGTGGTCGACAAAGTGAACGACTCAACGTTTCACAACA
TGAAGGAGCTGCGCCCCGCCGGCACCAGCATCCGGATCCTGTTCGCCTTCGACCCGGCCCGGCAGGCGATCCTGCTGCTG
GGCGGTGACAAGGCAGGCAACTGGAAACGCTGGTACGACAACAACATTCCAATCGCTGACCAGCGCTCCGAGAACTGGCT
GGCGAGCGAGCACGGAGGTGGATGA

Antitoxin


Download         Length: 110 a.a.        Molecular weight: 11802.46 Da        Isoelectric Point: 7.4051

>AT83663 WP_003899955.1 NZ_CP023605:3550661-3550990 [Mycobacterium tuberculosis]
MTMARNWRDIRADAVAQGRVDLQRAAVAREEMRDAVLAHRLAEIRKALGHARQADVAALMGVSQARVSKLESGDLSHTEL
GTLQAYVAALGGHLRIVAEFGENTVELTA

Download         Length: 330 bp

>AT83663 NZ_CP023605:3550661-3550990 [Mycobacterium tuberculosis]
ATGACCATGGCCCGCAACTGGCGTGACATTCGCGCCGATGCCGTCGCGCAGGGCCGCGTGGATCTGCAGCGGGCCGCCGT
GGCACGCGAGGAGATGCGCGATGCCGTCCTGGCGCACCGCCTGGCCGAGATCCGCAAGGCGCTAGGCCACGCACGTCAGG
CCGACGTCGCGGCGCTGATGGGGGTCTCTCAGGCCCGTGTCTCCAAGCTGGAGAGCGGCGACCTGTCCCACACCGAACTC
GGCACCCTGCAGGCCTACGTTGCCGCCCTGGGCGGGCACCTGCGCATCGTCGCTGAGTTCGGCGAAAATACTGTCGAGCT
GACCGCCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U4FBK2

Antitoxin

Source ID Structure
PDB 6LTY
PDB 6LTZ
AlphaFold DB G0THF6

References