166374

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 3577656..3578326 Replicon chromosome
Accession NZ_CP058236
Organism Mycobacterium tuberculosis strain 060827

Toxin (Protein)


Gene name higB Uniprot ID O53332
Locus tag HWS75_RS16930 Protein ID WP_003899954.1
Coordinates 3577656..3578000 (+) Length 115 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID G0THF6
Locus tag HWS75_RS16935 Protein ID WP_003899955.1
Coordinates 3577997..3578326 (+) Length 110 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HWS75_RS16900 3572737..3572948 + 212 Protein_3337 (R)-hydratase -
HWS75_RS16905 3572961..3573254 + 294 WP_003416635.1 hypothetical protein -
HWS75_RS16910 3573542..3574732 + 1191 Protein_3339 ATP-binding protein -
HWS75_RS16920 3576536..3576970 - 435 WP_003899952.1 type II toxin-antitoxin system VapC family toxin -
HWS75_RS16925 3576973..3577425 - 453 WP_003899953.1 type II toxin-antitoxin system Phd/YefM family antitoxin -
HWS75_RS16930 3577656..3578000 + 345 WP_003899954.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
HWS75_RS16935 3577997..3578326 + 330 WP_003899955.1 XRE family transcriptional regulator Antitoxin
HWS75_RS16945 3580223..3580570 + 348 WP_003899956.1 DUF2384 domain-containing protein -
HWS75_RS16950 3580567..3581187 + 621 WP_003899957.1 RES family NAD+ phosphorylase -
HWS75_RS16955 3581347..3582612 - 1266 WP_003909837.1 hypothetical protein -
HWS75_RS16960 3582780..3582899 + 120 Protein_3349 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - - 3574774..3599832 25058
- inside IScluster/Tn - - 3574774..3584199 9425


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-103)

Antitoxin

Cro/C1-type helix-turn-helix domain

(38-91)


Sequences

Toxin        


Download         Length: 115 a.a.        Molecular weight: 12692.50 Da        Isoelectric Point: 5.6920

>T166374 WP_003899954.1 NZ_CP058236:3577656-3578000 [Mycobacterium tuberculosis]
MAVILLPQVERWFFALNRDAMASVTGAIDLLEMEGPTLGRPVVDKVNDSTFHNMKELRPAGTSIRILFAFDPARQAILLL
GGDKAGNWKRWYDNNIPIADQRSENWLASEHGGG

Download         Length: 345 bp

>T166374 NZ_CP074141:c896-564 [Serratia sp. JSRIV004]
ATGGTTAAAGGTTATGTTCCTGACGCCGGTGATATTGTCTGGTTAGAGTTTGATCCCCAGGCAGGGCATGAACAAGCGGG
CCATCGCCCTGCTTTAGTGCTCAGTCCCGCTGTATACAACGGGCGGATTGGGTTAATGCTGTGCTGCCCGATGACAACCA
AAATCAAAGGGTATCCTTTTGAAGTCATTGTCCCTGGTGAACGTGAGAGTGCAGTCCTTGCCGATCAAGTTAAGAGCCTG
GATTGGAAAGAACGTAATGCAACAAAAAAGGGACAGGTACCTGCGTCGGTACTGTCGGATGTTCGTGCAAAGGCAAAAGC
CTTAATAGGGTAA

Antitoxin


Download         Length: 110 a.a.        Molecular weight: 11802.46 Da        Isoelectric Point: 7.4051

>AT166374 WP_003899955.1 NZ_CP058236:3577997-3578326 [Mycobacterium tuberculosis]
MTMARNWRDIRADAVAQGRVDLQRAAVAREEMRDAVLAHRLAEIRKALGHARQADVAALMGVSQARVSKLESGDLSHTEL
GTLQAYVAALGGHLRIVAEFGENTVELTA

Download         Length: 330 bp

>AT166374 NZ_CP074141:c1141-896 [Serratia sp. JSRIV004]
ATGTCTCAAGTTGTGGTTAAAAAATGGGGTAACAGCCCGTCAGTCCGTCTTCCTGTCGCCGTCATGAAAGCAGCGTCGCT
CAACGTGGATGATACCGTTGAGGTGAGTGTCGAAGAAGGGCGTATCGTCATTGTACCGGTGCGAGCCAAGGTCTATTCAC
TAGACGGCTTGCTGGCAGGCGTGACTGACGAGAATATTCACCAGGCAGTGTCGTTTGGTGAGCCTGTTGGCAAAGAGGCA
CTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U4FBK2

Antitoxin

Source ID Structure
PDB 6LTY
PDB 6LTZ
AlphaFold DB G0THF6

References