Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 3550370..3551040 Replicon chromosome
Accession NZ_CP071128
Organism Mycobacterium tuberculosis strain 267/47W148

Toxin (Protein)


Gene name higB Uniprot ID O53332
Locus tag JZL63_RS16635 Protein ID WP_003899954.1
Coordinates 3550370..3550714 (+) Length 115 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID G0THF6
Locus tag JZL63_RS16640 Protein ID WP_003899955.1
Coordinates 3550711..3551040 (+) Length 110 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JZL63_RS16605 3545443..3546303 + 861 WP_003416628.1 alpha/beta hydrolase -
JZL63_RS16610 3546278..3546793 + 516 WP_003899950.1 nitroreductase family deazaflavin-dependent oxidoreductase -
JZL63_RS16615 3547033..3547326 + 294 WP_003416635.1 hypothetical protein -
JZL63_RS16620 3547614..3548903 + 1290 WP_003416640.1 ATP-binding protein -
JZL63_RS16625 3549250..3549684 - 435 WP_003899952.1 type II toxin-antitoxin system VapC family toxin -
JZL63_RS16630 3549687..3550139 - 453 WP_003899953.1 type II toxin-antitoxin system Phd/YefM family antitoxin -
JZL63_RS16635 3550370..3550714 + 345 WP_003899954.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
JZL63_RS16640 3550711..3551040 + 330 WP_003899955.1 XRE family transcriptional regulator Antitoxin
JZL63_RS16645 3551277..3552538 + 1262 WP_087902221.1 IS3-like element IS987 family transposase -
JZL63_RS16650 3552760..3554021 + 1262 WP_087902221.1 IS3-like element IS987 family transposase -
JZL63_RS16655 3554294..3554641 + 348 WP_003899956.1 DUF2384 domain-containing protein -
JZL63_RS16660 3554638..3555258 + 621 WP_003899957.1 RES family NAD+ phosphorylase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - - 3547614..3580491 32877


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-103)

Antitoxin

Cro/C1-type helix-turn-helix domain

(38-91)


Sequences

Toxin        


Download         Length: 115 a.a.        Molecular weight: 12692.50 Da        Isoelectric Point: 5.6920

>T195445 WP_003899954.1 NZ_CP071128:3550370-3550714 [Mycobacterium tuberculosis]
MAVILLPQVERWFFALNRDAMASVTGAIDLLEMEGPTLGRPVVDKVNDSTFHNMKELRPAGTSIRILFAFDPARQAILLL
GGDKAGNWKRWYDNNIPIADQRSENWLASEHGGG

Download         Length: 345 bp

>T195445 NZ_CP094724:801707-802069 [Staphylococcus hominis]
ATGATTAGAAGAGGCGATGTATATTTAGCTGATTTATCACCAGTTCAAGGGTCTGAACAAGGGGGAGTAAGACCTGTAGT
TATCATTCAAAATGATACTGGTAATAAATATAGTCCTACAGTAATCGTTGCTGCGATTACTGGCAGGATTAATAAAGCGA
AAATACCAACACATGTAGAAATAGAAAAGAAAAAATATCGTTTAGATAAAGATTCAGTCATTCTTTTAGAACAAATACGA
ACATTGGATAAAAAAAGGTTGAAAGAGAAACTTACATATTTATCAGAAGATAAAATGAAAGAAGTTGACGATGCATTGGA
TATCAGTTTAGGTTTACACGATGTTCGTCCTCAAAAAAATTAG

Antitoxin


Download         Length: 110 a.a.        Molecular weight: 11802.46 Da        Isoelectric Point: 7.4051

>AT195445 WP_003899955.1 NZ_CP071128:3550711-3551040 [Mycobacterium tuberculosis]
MTMARNWRDIRADAVAQGRVDLQRAAVAREEMRDAVLAHRLAEIRKALGHARQADVAALMGVSQARVSKLESGDLSHTEL
GTLQAYVAALGGHLRIVAEFGENTVELTA

Download         Length: 330 bp

>AT195445 NZ_CP094724:801540-801710 [Staphylococcus hominis]
ATGTTAACTTTTAATCAGAATAGAAGCCATAGTCTTGAACAATCACTAAAAGAAGGCTATGTCCAAATGGCCGATTTAAA
CCTCTCCCTAGCAACTGAAGCATTTCCATTAGAATGTGAAGCTTGTGATTGTAACGAATCTTATCTTACATCTAATTATA
AAAGTGAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U4FBK2

Antitoxin

Source ID Structure
PDB 6LTY
PDB 6LTZ
AlphaFold DB G0THF6

References